α-2,6-Sialyltransferase, Pasteurella multocida (P-1059)
Based on 1 Customer Validation
alpha-2,6-Sialyltransferase (Pd26ST) (EC 2.4.99.1) is capable of sialylating both terminal and internal galactose and N-acetylgalactosamine, which producing terminal α2,6-sialosides.
For research use only. We do not sell to patients.
- CAS No.: 9075-81-4
- Molecular Weight:54 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
MCE Validation Data
In Vitro
Product Information
This product is recombinantly expressed in Escherichia coli, featuring a C-terminal His tag. It has a molecular weight of approximately 54 kDa and a purity greater than 90% (SDS-PAGE). The storage buffer consists of 20 mM Tris-HCl at pH 8.0 and 10% glycerol.
Instructions for Use
1. Add substrate according to the reaction system, 5mM MgCl2, and appropriate buffer salt (such as Tris-HCl), adjust the pH to 8.5, and add appropriate amount of α-2,6-Sialyltransferase (according to the reaction requirements);
2. After mixing, incubate the sample at 37°C for 2-4h.
Note: When the system is amplified, the incubation time or enzyme amount needs to be adjusted according to the actual situation, and low-speed shaking can be used for mixing.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
EC Number
2.4.99.1
Enzyme Activity
≥5 U/mL
Unit Definition
One unit is defined as the amount of enzyme required per minute to convert 1μmol of Neu5Ac from UDP-Neu5Ac to lactose to form Neu5Ac-α2,6 lac at 37 ° C.
Technical Parameters
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Source
E. coli
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Tag
C-terminal His Tag
Chemical Information
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CAS No. 9075-81-4
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Appearance Liquid
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Molecular Weight 54 kDa
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Color Colorless to light yellow
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SMILES
[α-2,6-Sialyltransferase, Pasteurella multocida (P-1059)]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
α-2,6-Sialyltransferase, Pasteurella multocida (P-1059) Related Classifications
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