2. Vitamin D Related/Nuclear Receptor
    Metabolic Enzyme/Protease
  3. Estrogen Receptor/ERR
    LXR
    Endogenous Metabolite
  4. 27-Hydroxycholesterol

27-Hydroxycholesterol 

製品番号: HY-N2371 純度: 99.38%
COA 取扱説明書

27-Hydroxycholesterol is a selective estrogen receptor modulator and an agonist of the liver X receptor.

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27-Hydroxycholesterol 構造式

27-Hydroxycholesterol 構造式

CAS 番号 : 20380-11-4

容量 価格（税別） 在庫状況 数量
Solution
10 mM * 1 mL in Ethanol USD 231 在庫あり
Estimated Time of Arrival: December 31
Solid
1 mg USD 60 在庫あり
Estimated Time of Arrival: December 31
5 mg USD 210 在庫あり
Estimated Time of Arrival: December 31
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50 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Estrogen receptor ERα ERβ ERRα ERRβ ERRγ

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製品説明

27-Hydroxycholesterol is a selective estrogen receptor modulator and an agonist of the liver X receptor.

IC50 & Target

Human Endogenous Metabolite

 

体外実験

27-Hydroxycholesterol is an endogenous selective estrogen receptor modulator that displays significant partial agonist activity in a variety of cellular models of estrogen receptor action. It positively regulates both gene transcription and cell proliferation in cellular models of breast cancer[1]. 27-Hydroxycholesterol, through estrogen receptor activation, triggers deleterious effect in prostate cancer cell lines. 27-Hydroxycholesterol significantly increases cell proliferation of LNCaP and PC3 cells and this effect can be attenuated by estrogen receptor inhibitors[2]. 27-Hydroxycholesterol is an oxysterol produced from cholesterol by the monooxygenase CYP27A1, which regulates intracellular cholesterol homeostasis. 27-Hydroxycholesterol also acts as an endogenous selective estrogen receptor modulator capable of increasing breast cancer growth and metastasis. 27-Hydroxycholesterol levels can be modulated by statins or direct inhibition of CYP27A1, thereby attenuating its pro-tumorigenic activities[3]. 27-hydroxylation of cholesterol is an important pathway for LXR activation in response to cholesterol overload[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

402.65

Appearance

Solid

分子式

C27H46O2
CAS 番号
SMILES
Structure Classification
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

Ethanol : 16.67 mg/mL (41.40 mM; Need ultrasonic)

DMSO : 1 mg/mL (2.48 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4177 mL 24.8355 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL
10 mM 0.2484 mL 1.2418 mL 2.4835 mL
*Please refer to the solubility information to select the appropriate solvent.
体内:
  • 1.

    Add each solvent one by one:  10% EtOH    40% PEG300    5% Tween-80    45% saline

    Solubility: 1 mg/mL (2.48 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% EtOH    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 1 mg/mL (2.48 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% EtOH    90% corn oil

    Solubility: ≥ 1 mg/mL (2.48 mM); Clear solution

  • 4.

    Add each solvent one by one:  10% EtOH    90% corn oil

    Solubility: ≥ 1 mg/mL (2.48 mM); Clear solution

*All of the co-solvents are available by MCE.
純度とドキュメンテーション

純度: 99.38%

COA (248 KB) HNMR (127 KB) RP-HPLC (86 KB)

取扱説明書 (2659 KB)
参考文献
細胞実験

Stock solutions of 27-Hydroxycholesterol are prepared in 100% ethanol and stored at −80°C. 27-Hydroxycholesterol stock solution is dissolved in appropriate volumes of media to prepare the working solutions of 1 μM.

MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。
動物実験
[2]

Proliferation assays are conducted on black 96 well plates using a commercial kit which quantifies cell number using DNA content and membrane integrity. LNCaP and PC3 cells are treated with 1 µM 27-Hydroxycholesterol for 48 hours[2].

MCE はこれらの方法の精度を確認していません。 こちらは参照専用です。
参考文献
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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

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Keywords:

27-Hydroxycholesterol20380-11-4Estrogen Receptor/ERRLXREndogenous MetaboliteLiver X receptorInhibitorinhibitorinhibit

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製品名:
27-Hydroxycholesterol
製品番号:
HY-N2371
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