7-Dehydrocholesterol

Name: 7-Dehydrocholesterol
Brand: MedChemExpress
SKU: HY-113279
Rating: 5.0 (7 reviews)

Cat. No.: HY-113279 Purity: 99.97%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

7-Dehydrocholesterol is biosynthetic precursor of cholesterol and vitamin D₃.

For research use only. We do not sell to patients.

CAS No. : 434-16-2

Size	Stock	Quantity
Solution
10 mM * 1 mL in Ethanol	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of 7-Dehydrocholesterol:

7-Dehydrocholesterol-d₇ In-stock
7-Dehydrocholesterol (Standard) In-stock

7 Publications Citing Use of MCE 7-Dehydrocholesterol

•Research Square Preprint. 2023 Jun 22.

Cell Imaging/Staining

Cell Proliferation/Viability Assay

: 7-Dehydrocholesterol purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2025 Nov 29:794:153070. [Abstract]; Cell viability of MuSCs with different concentrations of 7-Dehydrocholesterol (7-DHC).

: 7-Dehydrocholesterol purchased from MedChemExpress. Usage Cited in: Biochem Biophys Res Commun. 2025 Nov 29:794:153070. [Abstract]; The protein level of CCL2 in MuSCs after pre-treatment of MuSCs with 7-Dehydrocholesterol (7-DHC) (in a co-culture system).

: 7-Dehydrocholesterol purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2024 May 16;31(5):920-931.e6. [Abstract]; Mouse naive CD8+ T cells were transiently treated with 1 or 2 ng/μL 7-Dehydrocholesterol (7a-HC) in A and 2 ng/μL 7a-HC for 20 min.

: 7-Dehydrocholesterol purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2024 May 16;31(5):920-931.e6. [Abstract]; Effects of transient 7-Dehydrocholesterol (7a-HC) treatment on the phosphorylation levels of TCR and downstream molecules in mouse T cells. Mouse naive CD4+ T cells were transiently treated with 2 ng/μL 7a-HC for 20 min and then stimulated with 5 ng/μL α-CD3ε and 5 ng/μL α-CD28.

: 7-Dehydrocholesterol purchased from MedChemExpress. Usage Cited in: Cell Chem Biol. 2024 May 16;31(5):920-931.e6. [Abstract]; Naïve mouse CD8+ T cells were treated with 10 ng/μL cholesterol, 2 ng/μL 7-Dehydrocholesterol (7a-HC) for 20 min, or without any treatment. The plasma membrane was then labeled with octadecylrhodamine B (R18).

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Purity & Documentation
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Description

7-Dehydrocholesterol is biosynthetic precursor of cholesterol and vitamin D₃.

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

7-Dehydrocholesterol is a biosynthetic precursor of cholesterol and vitamin D₃. 7-Dehydrocholesterol is present in relatively high concentration in skin where it is converted to pre-vitamin D₃ upon UV irradiation and where it is also exposed to exogenous radical sources and oxygen^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

384.64

Formula

C₂₇H₄₄O

CAS No.

434-16-2

Appearance

Solid

Color

White to light yellow

SMILES

CC(C)CCC[C@@H](C)[C@H]1CC[C@@]2([H])C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@]3([H])CC[C@]12C

Structure Classification

Steroids

Initial Source

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

Ethanol : 12.5 mg/mL (32.50 mM; Need ultrasonic)

DMSO : < 1 mg/mL (insoluble or slightly soluble)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5998 mL	12.9992 mL	25.9983 mL
5 mM	0.5200 mL	2.5998 mL	5.1997 mL
10 mM	0.2600 mL	1.2999 mL	2.5998 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 0.83 mg/mL (2.16 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (8.3 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 0.83 mg/mL (2.16 mM); Clear solution

This protocol yields a clear solution of ≥ 0.83 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (8.3 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.97%

Select Batch:

Data Sheet (272 KB) SDS (393 KB)

COA (248 KB) HNMR (262 KB) RP-HPLC (206 KB) MS (82 KB)

Handling Instructions (2659 KB)

References

[1]. Xu L, et al. Oxysterols from free radical chain oxidation of 7-dehydrocholesterol: product and mechanistic studies. J Am Chem Soc. 2010 Feb 24;132(7):2222-32. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

Ethanol	1 mM	2.5998 mL	12.9992 mL	25.9983 mL	64.9958 mL
	5 mM	0.5200 mL	2.5998 mL	5.1997 mL	12.9992 mL
	10 mM	0.2600 mL	1.2999 mL	2.5998 mL	6.4996 mL
	15 mM	0.1733 mL	0.8666 mL	1.7332 mL	4.3331 mL
	20 mM	0.1300 mL	0.6500 mL	1.2999 mL	3.2498 mL
	25 mM	0.1040 mL	0.5200 mL	1.0399 mL	2.5998 mL
	30 mM	0.0867 mL	0.4333 mL	0.8666 mL	2.1665 mL