1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. 25-Hydroxycholesterol

25-Hydroxycholesterol 

Cat. No.: HY-113134 Purity: ≥98.0%
Handling Instructions

25-Hydroxycholesterol is a metabolite of cholesterol that is produced and secreted by macrophages in response to Toll-like receptor (TLR) activation. 25-hydroxycholesterol is a potent (EC50≈65 nM) and selective suppressor of IgA production by B cells.

For research use only. We do not sell to patients.

25-Hydroxycholesterol Chemical Structure

25-Hydroxycholesterol Chemical Structure

CAS No. : 2140-46-7

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Free Sample (0.5-1 mg)   Apply Now  
Solution
10 mM * 1 mL in DMSO USD 61 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 61 In-stock
Estimated Time of Arrival: December 31
Solid
5 mg USD 55 In-stock
Estimated Time of Arrival: December 31
10 mg USD 77 In-stock
Estimated Time of Arrival: December 31
25 mg USD 121 In-stock
Estimated Time of Arrival: December 31
50 mg USD 187 In-stock
Estimated Time of Arrival: December 31
100 mg USD 297 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

Based on 9 publication(s) in Google Scholar

Other Forms of 25-Hydroxycholesterol:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

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Description

25-Hydroxycholesterol is a metabolite of cholesterol that is produced and secreted by macrophages in response to Toll-like receptor (TLR) activation. 25-hydroxycholesterol is a potent (EC50≈65 nM) and selective suppressor of IgA production by B cells.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

The synthesis of 25-hydroxycholesterol is catalyzed by the enzyme cholesterol 25-hydroxylase, which uses cholesterol and molecular oxygen as substrates and NADPH as a cofactor. 25-hydroxycholesterol is a potent bioactive lipid in the innate and adaptive immune systems[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

402.65

Formula

C27H46O2

CAS No.
SMILES
Structure Classification
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 14.29 mg/mL (35.49 mM; Need ultrasonic)

DMSO : 5 mg/mL (12.42 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4177 mL 24.8355 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL
10 mM 0.2484 mL 1.2418 mL 2.4835 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  50% PEG300    50% saline

    Solubility: 10 mg/mL (24.84 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.5 mg/mL (1.24 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 0.5 mg/mL (1.24 mM); Clear solution

*All of the co-solvents are available by MCE.
References

Purity: ≥98.0%

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
25-Hydroxycholesterol
Cat. No.:
HY-113134
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