1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. 25-Hydroxycholesterol

25-Hydroxycholesterol 

Cat. No.: HY-113134 Purity: >98.0%
Handling Instructions

25-Hydroxycholesterol is a metabolite of cholesterol that is produced and secreted by macrophages in response to Toll-like receptor (TLR) activation. 25-hydroxycholesterol is a potent (EC50≈65 nM) and selective suppressor of IgA production by B cells.

For research use only. We do not sell to patients.

25-Hydroxycholesterol Chemical Structure

25-Hydroxycholesterol Chemical Structure

CAS No. : 2140-46-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
5 mg USD 50 In-stock
Estimated Time of Arrival: December 31
10 mg USD 70 In-stock
Estimated Time of Arrival: December 31
25 mg USD 110 In-stock
Estimated Time of Arrival: December 31
50 mg USD 170 In-stock
Estimated Time of Arrival: December 31
100 mg USD 270 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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Description

25-Hydroxycholesterol is a metabolite of cholesterol that is produced and secreted by macrophages in response to Toll-like receptor (TLR) activation. 25-hydroxycholesterol is a potent (EC50≈65 nM) and selective suppressor of IgA production by B cells.

In Vitro

The synthesis of 25-hydroxycholesterol is catalyzed by the enzyme cholesterol 25-hydroxylase, which uses cholesterol and molecular oxygen as substrates and NADPH as a cofactor. 25-hydroxycholesterol is a potent bioactive lipid in the innate and adaptive immune systems[1].

Molecular Weight

402.65

Formula

C₂₇H₄₆O₂

CAS No.

2140-46-7

SMILES
Shipping

Room temperature in continental US; may vary elsewhere

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 14.29 mg/mL (35.49 mM; Need ultrasonic)

DMSO : 5 mg/mL (12.42 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4177 mL 24.8355 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL
10 mM 0.2484 mL 1.2418 mL 2.4835 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 0.5 mg/mL (1.24 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 0.5 mg/mL (1.24 mM); Clear solution

*All of the co-solvents are provided by MCE.
References

Purity: >98.0%

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Product Name:
25-Hydroxycholesterol
Cat. No.:
HY-113134
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