1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite

25-Hydroxycholesterol 

Cat. No.: HY-113134
Handling Instructions

25-Hydroxycholesterol is a metabolite of cholesterol that is produced and secreted by macrophages in response to Toll-like receptor (TLR) activation. 25-hydroxycholesterol is a potent (EC50≈65 nM) and selective suppressor of IgA production by B cells.

For research use only. We do not sell to patients.

25-Hydroxycholesterol Chemical Structure

25-Hydroxycholesterol Chemical Structure

CAS No. : 2140-46-7

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 55 In-stock
Estimated Time of Arrival: December 31
5 mg USD 50 In-stock
Estimated Time of Arrival: December 31
10 mg USD 70 In-stock
Estimated Time of Arrival: December 31
25 mg USD 110 In-stock
Estimated Time of Arrival: December 31
50 mg USD 170 In-stock
Estimated Time of Arrival: December 31
100 mg USD 270 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

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  • Biological Activity

  • Technical Information

  • Purity & Documentation

  • References

Description

25-Hydroxycholesterol is a metabolite of cholesterol that is produced and secreted by macrophages in response to Toll-like receptor (TLR) activation. 25-hydroxycholesterol is a potent (EC50≈65 nM) and selective suppressor of IgA production by B cells.

In Vitro

The synthesis of 25-hydroxycholesterol is catalyzed by the enzyme cholesterol 25-hydroxylase, which uses cholesterol and molecular oxygen as substrates and NADPH as a cofactor. 25-hydroxycholesterol is a potent bioactive lipid in the innate and adaptive immune systems[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4177 mL 24.8355 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL
10 mM 0.2484 mL 1.2418 mL 2.4835 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Molecular Weight

402.65

Formula

C₂₇H₄₆O₂

CAS No.

2140-46-7

SMILES

CC(C)(O)CCC[[email protected]@H](C)[[email protected]]1CC[[email protected]@]2([H])[[email protected]]3([H])CC=C4C[[email protected]@H](O)CC[[email protected]]4(C)[[email protected]@]3([H])CC[[email protected]]12C

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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This equation is commonly abbreviated as: C1V1 = C2V2

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× = ×
C1   V1   C2   V2

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Product Name:
25-Hydroxycholesterol
Cat. No.:
HY-113134
Quantity:

25-Hydroxycholesterol

Cat. No.: HY-113134