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    Metabolic Enzyme/Protease
  2. Estrogen Receptor/ERR
    LXR
    Endogenous Metabolite
  3. 27-Hydroxycholesterol

27-Hydroxycholesterol 

Cat. No.: HY-N2371 Purity: 99.38%
Handling Instructions

27-Hydroxycholesterol is a selective estrogen receptor modulator and an agonist of the liver X receptor.

For research use only. We do not sell to patients.

27-Hydroxycholesterol Chemical Structure

27-Hydroxycholesterol Chemical Structure

CAS No. : 20380-11-4

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1 mg USD 72 In-stock
Estimated Time of Arrival: December 31
5 mg USD 252 In-stock
Estimated Time of Arrival: December 31
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Description

27-Hydroxycholesterol is a selective estrogen receptor modulator and an agonist of the liver X receptor.

IC50 & Target

Human Endogenous Metabolite

 

In Vitro

27-Hydroxycholesterol is an endogenous selective estrogen receptor modulator that displays significant partial agonist activity in a variety of cellular models of estrogen receptor action. It positively regulates both gene transcription and cell proliferation in cellular models of breast cancer[1]. 27-Hydroxycholesterol, through estrogen receptor activation, triggers deleterious effect in prostate cancer cell lines. 27-Hydroxycholesterol significantly increases cell proliferation of LNCaP and PC3 cells and this effect can be attenuated by estrogen receptor inhibitors[2]. 27-Hydroxycholesterol is an oxysterol produced from cholesterol by the monooxygenase CYP27A1, which regulates intracellular cholesterol homeostasis. 27-Hydroxycholesterol also acts as an endogenous selective estrogen receptor modulator capable of increasing breast cancer growth and metastasis. 27-Hydroxycholesterol levels can be modulated by statins or direct inhibition of CYP27A1, thereby attenuating its pro-tumorigenic activities[3]. 27-hydroxylation of cholesterol is an important pathway for LXR activation in response to cholesterol overload[4].

Molecular Weight

402.65

Formula

C₂₇H₄₆O₂

CAS No.

20380-11-4

SMILES
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

Ethanol : 10 mg/mL (24.84 mM; Need ultrasonic)

DMSO : 1 mg/mL (2.48 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.4835 mL 12.4177 mL 24.8355 mL
5 mM 0.4967 mL 2.4835 mL 4.9671 mL
10 mM 0.2484 mL 1.2418 mL 2.4835 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% EtOH    90% corn oil

    Solubility: ≥ 1 mg/mL (2.48 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay

Stock solutions of 27-Hydroxycholesterol are prepared in 100% ethanol and stored at −80°C. 27-Hydroxycholesterol stock solution is dissolved in appropriate volumes of media to prepare the working solutions of 1 μM.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Proliferation assays are conducted on black 96 well plates using a commercial kit which quantifies cell number using DNA content and membrane integrity. LNCaP and PC3 cells are treated with 1 µM 27-Hydroxycholesterol for 48 hours[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.38%

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Keywords:

27-HydroxycholesterolEstrogen Receptor/ERRLXREndogenous MetaboliteLiver X receptorInhibitorinhibitorinhibit

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27-Hydroxycholesterol
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