3'-O-(2-Nitrobenzyl)-dCTP 

3'-O-(2-Nitrobenzyl)-dCTP is a reversible terminator. 3'-O-(2-Nitrobenzyl)-dCTP can be recognized and incorporated by polymerases, thereby temporarily terminating primer extension during DNA synthesis. After incorporation into the extending DNA strand, 3'-O-(2-Nitrobenzyl)-dCTP temporarily halts the DNA polymerase extension reaction, and its 3'-O-(2-nitrobenzyl) blocking group can be removed by laser irradiation to regenerate a free 3'-OH for subsequent primer extension cycles. 3'-O-(2-Nitrobenzyl)-dCTP is applicable for resolving homopolymeric regions in DNA templates^[1].

3'-O-(2-Nitrobenzyl)-dCTP (100 pmol) is efficiently and quantitatively incorporated as a substrate by 9°N polymerase (exo-)A485L/Y409V in in vitro DNA extension reactions, forming a complete primer extension product^[1].
3'-O-(2-Nitrobenzyl)-dCTP (200 pmol) functions as an effective reversible terminator for pyrosequencing, allowing accurate, single-base resolution deciphering of homopolymeric DNA sequences when used in extension-signal detection-deprotection cycles^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

602.28

C₁₆H₂₁N₄O₁₅P₃

959755-19-2

OP(OC[C@H]1O[C@@H](N2C(N=C(N)C=C2)=O)C[C@@H]1OCC3=CC=CC=C3[N+]([O-])=O)(OP(OP(O)(O)=O)(O)=O)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Wu J, et al. 3'-O-modified nucleotides as reversible terminators for pyrosequencing. Proc Natl Acad Sci U S A. 2007;104(42):16462-16467.  [Content Brief]