3'-O-(2-Nitrobenzyl)-dCTP
3'-O-(2-Nitrobenzyl)-dCTP is a reversible terminator. 3'-O-(2-Nitrobenzyl)-dCTP can be recognized and incorporated by polymerases, thereby temporarily terminating primer extension during DNA synthesis. After incorporation into the extending DNA strand, 3'-O-(2-Nitrobenzyl)-dCTP temporarily halts the DNA polymerase extension reaction, and its 3'-O-(2-nitrobenzyl) blocking group can be removed by laser irradiation to regenerate a free 3'-OH for subsequent primer extension cycles. 3'-O-(2-Nitrobenzyl)-dCTP is applicable for resolving homopolymeric regions in DNA templates.
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- CAS No.: 959755-19-2
- Formula: C16H21N4O15P3
- Molecular Weight:602.28
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Help & FAQs
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Biological Activity
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DNA Polymerase |
3'-O-(2-Nitrobenzyl)-dCTP (100 pmol) is efficiently and quantitatively incorporated as a substrate by 9°N polymerase (exo-)A485L/Y409V in in vitro DNA extension reactions, forming a complete primer extension product[1].
3'-O-(2-Nitrobenzyl)-dCTP (200 pmol) functions as an effective reversible terminator for pyrosequencing, allowing accurate, single-base resolution deciphering of homopolymeric DNA sequences when used in extension-signal detection-deprotection cycles[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 959755-19-2
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Molecular Weight 602.28
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Formula C16H21N4O15P3
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SMILES
OP(OC[C@H]1O[C@@H](N2C(N=C(N)C=C2)=O)C[C@@H]1OCC3=CC=CC=C3[N+]([O-])=O)(OP(OP(O)(O)=O)(O)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
3'-O-(2-Nitrobenzyl)-dCTP Related Classifications
HY-185272 Related Classifications
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