4-Chloro-3-nitrobenzoic acid
Based on 1 Customer Validation
4-Chloro-3-nitrobenzoic acid is a benzoic acid derivative and an important intermediate for the synthesis of anticancer agents. 4-Chloro-3-nitrobenzoic acid shows low cytotoxicity against HepG2, A549 and HeLa cancer cells in vitro (>10,000 μM). 4-Chloro-3-nitrobenzoic acid can block thymidine uptake and inhibit lymphocyte growth. 4-Chloro-3-nitrobenzoic acid serves as a ligand for Cu (II) complexes and is used in the preparation of 10H-pyrazino[2,3-b][1,4]benzothiazine derivatives.
For research use only. We do not sell to patients.
- Purity: 99.39%
- CAS No.: 96-99-1
- Formula: C7H4ClNO4
- Molecular Weight:201.56
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
4-Chloro-3-nitrobenzoic acid (5-1000 μM; 3 d) blocks anti-CD3-induced proliferation of H9 lymphoblastoid cells in a concentration-dependent manner[1].
4-Chloro-3-nitrobenzoic acid (48 h) lacks in vitro antiproliferative activity against HepG2, HeLa, and A549 cancer cell lines, with IC50 values >1000 μM against HepG2 and >10,000 μM against HeLa and A549 cells after 48 h incubation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:H9 lymphoblastoid cells
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Concentration:5, 10, 100, 1000 μM
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Incubation Time:3 days
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Result:Blocked [methyl-3H] thymidine uptake (a measure of DNA synthesis and cell proliferation) in a concentration-dependent manner.
Decreased uptake as the concentration increased from 5 to 1000 μM.
Chemical Information
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CAS No. 96-99-1
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Appearance Solid
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Molecular Weight 201.56
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Formula C7H4ClNO4
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Color White to off-white
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SMILES
O=C(O)C1=CC=C(Cl)C([N+]([O-])=O)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 200 mg/mL (992.26 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (24.81 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (24.81 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.9613 mL | 24.8065 mL | 49.6130 mL | 124.0325 mL |
| 5 mM | 0.9923 mL | 4.9613 mL | 9.9226 mL | 24.8065 mL | |
| 10 mM | 0.4961 mL | 2.4807 mL | 4.9613 mL | 12.4033 mL | |
| 15 mM | 0.3308 mL | 1.6538 mL | 3.3075 mL | 8.2688 mL | |
| 20 mM | 0.2481 mL | 1.2403 mL | 2.4807 mL | 6.2016 mL | |
| 25 mM | 0.1985 mL | 0.9923 mL | 1.9845 mL | 4.9613 mL | |
| 30 mM | 0.1654 mL | 0.8269 mL | 1.6538 mL | 4.1344 mL | |
| 40 mM | 0.1240 mL | 0.6202 mL | 1.2403 mL | 3.1008 mL | |
| 50 mM | 0.0992 mL | 0.4961 mL | 0.9923 mL | 2.4807 mL | |
| 60 mM | 0.0827 mL | 0.4134 mL | 0.8269 mL | 2.0672 mL | |
| 80 mM | 0.0620 mL | 0.3101 mL | 0.6202 mL | 1.5504 mL | |
| 100 mM | 0.0496 mL | 0.2481 mL | 0.4961 mL | 1.2403 mL |
4-Chloro-3-nitrobenzoic acid Related Classifications
HY-20559 Related Classifications
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