6-Azido-D-lysine hydrochloride
Based on 1 Customer Validation
6-Azido-D-lysine hydrochloride is a click chemistry reagent containing an azide. 6-Azido-D-lysine hydrochloride is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. It can also undergo strain-promoted alkyne-azide cycloaddition (SPAAC) reactions with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- Purity : ≥98.0%
- CAS No.: 2098497-01-7
- Formula: C6H13ClN4O2
- Molecular Weight:208.65
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Lactobacillus paracasei labeling[1]
1. Add 1 mM 6-Azido-D-lysine hydrochloride to 1 mL of Lactobacillus paracasei culture medium and incubate for 1 hour.
2. Collect the labeled bacteria by centrifugation (3000 × g, 2 minutes) and wash three times with PBS.
3. A 5 mM solution of DBCO-sulfo-Cy5 (HY-D1068) in PBS is added to the azide-modified bacteria and incubated for an additional 1 hour at room temperature at 100 rpm.
4. Metabolically labeled bacteria are also harvested by centrifugation and washed three times with PBS for further characterization.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2098497-01-7
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Appearance Solid
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Molecular Weight 208.65
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Formula C6H13ClN4O2
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Color White to light yellow
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SMILES
OC([C@H](N)CCCCN=[N+]=[N-])=O.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
In Vitro:
H2O : 116.67 mg/mL (559.17 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (268 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 4.7927 mL | 23.9636 mL | 47.9272 mL | 119.8179 mL |
| 5 mM | 0.9585 mL | 4.7927 mL | 9.5854 mL | 23.9636 mL | |
| 10 mM | 0.4793 mL | 2.3964 mL | 4.7927 mL | 11.9818 mL | |
| 15 mM | 0.3195 mL | 1.5976 mL | 3.1951 mL | 7.9879 mL | |
| 20 mM | 0.2396 mL | 1.1982 mL | 2.3964 mL | 5.9909 mL | |
| 25 mM | 0.1917 mL | 0.9585 mL | 1.9171 mL | 4.7927 mL | |
| 30 mM | 0.1598 mL | 0.7988 mL | 1.5976 mL | 3.9939 mL | |
| 40 mM | 0.1198 mL | 0.5991 mL | 1.1982 mL | 2.9954 mL | |
| 50 mM | 0.0959 mL | 0.4793 mL | 0.9585 mL | 2.3964 mL | |
| 60 mM | 0.0799 mL | 0.3994 mL | 0.7988 mL | 1.9970 mL | |
| 80 mM | 0.0599 mL | 0.2995 mL | 0.5991 mL | 1.4977 mL | |
| 100 mM | 0.0479 mL | 0.2396 mL | 0.4793 mL | 1.1982 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
6-Azido-D-lysine hydrochloride Related Classifications
HY-151666A Related Classifications
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