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  3. Cy5-DBCO

Cy5-DBCO  (Synonyms: DBCO-Sulfo-Cy5)

Cat. No.: HY-D1068 Purity: 98.93%
COA Handling Instructions

Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR)red fluorescent dye with λabsand λem of 646nm and 670 nm, respectively. Cy5-DBCO (DBCO-Sulfo-Cy5) is not suitable for staining intracellular components of permeabilezed cell, it may exhibits a high background. Cy5-DBCO is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

For research use only. We do not sell to patients.

Cy5-DBCO Chemical Structure

Cy5-DBCO Chemical Structure

CAS No. : 1564286-24-3

Size Price Stock Quantity
1 mg USD 285 In-stock
5 mg USD 850 In-stock
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Based on 1 publication(s) in Google Scholar

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Description

Cy5-DBCO (DBCO-Sulfo-Cy5) is a near-infrared (NIR)red fluorescent dye with λabsand λem of 646nm and 670 nm, respectively. Cy5-DBCO (DBCO-Sulfo-Cy5) is not suitable for staining intracellular components of permeabilezed cell, it may exhibits a high background. Cy5-DBCO is a click chemistry reagent, it contains a DBCO group that can undergo strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing Azide groups.

IC50 & Target

Red fluorescent dye

In Vitro

Cy5-DBCO (DBCO-Sulfo-Cy5) reacts with azides via a copper-free “click chemistry” reaction and forms a stable triazole and does not need Cu-catalyst or elevated temperatures.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1009.22

Formula

C52H56N4O11S3

CAS No.
Appearance

Solid

Color

Blue to black

Emission (Em)

668

Excitation (Ex)

647

SMILES

O=S(CCC[N+]1=C(/C=C/C=C/C=C(N(CCCCCC(NCCC(N2C3=CC=CC=C3C#CC4=CC=CC=C4C2)=O)=O)C5=CC=C(C=C65)S(=O)(O)=O)/C6(C)C)C(C)(C)C7=CC(S(=O)(O)=O)=CC=C17)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (61.93 mM; Need ultrasonic)

H2O : ≥ 10 mg/mL (9.91 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9909 mL 4.9543 mL 9.9086 mL
5 mM 0.1982 mL 0.9909 mL 1.9817 mL
10 mM 0.0991 mL 0.4954 mL 0.9909 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: 99.86%

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Cy5-DBCO Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cy5-DBCO
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HY-D1068
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