7α,27-Dihydroxycholesterol
Based on 1 Customer Validation
7α,27-Dihydroxycholesterol is an oxysterol, a human metabolite, and a ligand for EBI2 (GPR183), which is involved in lipid synthesis. 7α,27-Dihydroxycholesterol mediates the chemotaxis of cells expressing EBI2. 7α,27-Dihydroxycholesterol can be converted into 7α,27-dihydroxy-4-cholesten-3-one (HY-W414457), thereby inhibiting HMG-CoA reductase (HMGCR), the rate-limiting enzyme in cholesterol synthesis.
For research use only. We do not sell to patients.
- Purity: 99.93%
- CAS No.: 4725-24-0
- Formula: C27H46O3
- Molecular Weight:418.65
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Storage:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
7α,27-Dihydroxycholesterol (0.12 μM; 24 h) suppresses HMG-CoA reductase activity to 30% of control levels in normal human fibroblasts (line GM 08333)[2].
7α,27-Dihydroxycholesterol (0.12 μM; 24 h) suppresses HMG-CoA reductase activity to 60% of control levels in SV40 virus-transformed human fibroblasts (line 90-VA IV)[2].
7α,27-Dihydroxycholesterol (0.12 μM; 24 h) suppresses HMG-CoA reductase activity to 74% of control levels in human colonic carcinoma cells (line WiDr)[2].
7α,27-Dihydroxycholesterol (0.12 μM; 24 h) suppresses HMG-CoA reductase activity to 73% of control levels in human malignant melanoma cells (line SK-MEL-2)[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 4725-24-0
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Appearance Solid
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Molecular Weight 418.65
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Formula C27H46O3
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Color White to off-white
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SMILES
C[C@@]12[C@](CC[C@]2([H])[C@H](C)CCCC(C)CO)([H])[C@@]3([H])[C@@](CC1)([H])[C@@]4(C(C[C@H](CC4)O)=C[C@H]3O)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 10 mg/mL (23.89 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[2].
Axelson M, Larsson O. 27-hydroxylated low density lipoprotein (LDL) cholesterol can be converted to 7alpha,27-dihydroxy-4-cholesten-3-one (cytosterone) before suppressing cholesterol production in normal human fibroblasts. Evidence that an altered metabolism of ldl cholesterol can underlie a defective feedback control in malignant cells. J Biol Chem. 1996 May 31;271(22):12724-36.
[Content Brief]
[3]. Zhang YL, et al. Metabolic Dysregulation of 27-Hydroxycholesterol Sensitizes Proximal Tubular Epithelial Cells to Ferroptosis in Ischemic Acute Kidney Injury. Journal of the American Society of Nephrology : JASN. 2026 Mar 01;37(3):445-459. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3886 mL | 11.9432 mL | 23.8863 mL | 59.7158 mL |
| 5 mM | 0.4777 mL | 2.3886 mL | 4.7773 mL | 11.9432 mL | |
| 10 mM | 0.2389 mL | 1.1943 mL | 2.3886 mL | 5.9716 mL | |
| 15 mM | 0.1592 mL | 0.7962 mL | 1.5924 mL | 3.9811 mL | |
| 20 mM | 0.1194 mL | 0.5972 mL | 1.1943 mL | 2.9858 mL |
7α,27-Dihydroxycholesterol Related Classifications
HY-W414548 Related Classifications
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