7-Amino-4-(trifluoromethyl)coumarin
Based on 1 publication(s) in Google Scholar
7-Amino-4-(trifluoromethyl)coumarin (Coumarin 151) is a fluorescent marker for the sensitive detection of proteinases. The excitation and emission wavelengths are 400 and 490 nm, respectively.
For research use only. We do not sell to patients.
- Purity : 99.04%
- CAS No.: 53518-15-3
- Formula: C10H6F3NO2
- Molecular Weight:229.16
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) 7-Amino-4-(trifluoromethyl)coumarin
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Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| SK-MEL-28 | IC50 |
0.98 μM
Compound: 11
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Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
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[PMID: 37197457] |
| SK-MEL3 | IC50 |
0.93 μM
Compound: 11
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Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
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[PMID: 37197457] |
In Vitro
Amino acid and peptide derivatives of 7-Amino-4-(trifluoromethyl)coumarin are used to monitor peptidase activities[1].
7-Amino-4-(trifluoromethyl)coumarin fluoresces at neutral pH with excitation and emission wavelengths of 400 nm and 500 nm, respectively. The concentration and fluorescence show a linear relationship in the range of 10 to 300 pmol/3 mL of reaction mixture[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Emission (Em)
454
Excitation (Ex)
344
Chemical Information
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CAS No. 53518-15-3
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Appearance Solid
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Molecular Weight 229.16
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Formula C10H6F3NO2
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Color Light yellow to yellow
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SMILES
O=C1C=C(C(F)(F)F)C2=C(O1)C=C(N)C=C2
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Synonyms
Coumarin 151; AFC
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (1)
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Journal Impact Factor
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Most Recent
Solvent & Solubility
In Vitro:
DMSO : 100 mg/mL (436.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.3638 mL | 21.8188 mL | 43.6376 mL | 109.0941 mL |
| 5 mM | 0.8728 mL | 4.3638 mL | 8.7275 mL | 21.8188 mL | |
| 10 mM | 0.4364 mL | 2.1819 mL | 4.3638 mL | 10.9094 mL | |
| 15 mM | 0.2909 mL | 1.4546 mL | 2.9092 mL | 7.2729 mL | |
| 20 mM | 0.2182 mL | 1.0909 mL | 2.1819 mL | 5.4547 mL | |
| 25 mM | 0.1746 mL | 0.8728 mL | 1.7455 mL | 4.3638 mL | |
| 30 mM | 0.1455 mL | 0.7273 mL | 1.4546 mL | 3.6365 mL | |
| 40 mM | 0.1091 mL | 0.5455 mL | 1.0909 mL | 2.7274 mL | |
| 50 mM | 0.0873 mL | 0.4364 mL | 0.8728 mL | 2.1819 mL | |
| 60 mM | 0.0727 mL | 0.3636 mL | 0.7273 mL | 1.8182 mL | |
| 80 mM | 0.0545 mL | 0.2727 mL | 0.5455 mL | 1.3637 mL | |
| 100 mM | 0.0436 mL | 0.2182 mL | 0.4364 mL | 1.0909 mL |
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