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    Phenylpropanoids
  2. 7-Amino-4-(trifluoromethyl)coumarin

7-Amino-4-(trifluoromethyl)coumarin (Synonyms: Coumarin 151; AFC)

Cat. No.: HY-D0981 Purity: 98.92%
Handling Instructions

7-Amino-4-(trifluoromethyl)coumarin (Coumarin 151) is a fluorescent marker for the sensitive detection of proteinases. The excitation and emission wavelengths are 400 and 490 nm, respectively.

For research use only. We do not sell to patients.

7-Amino-4-(trifluoromethyl)coumarin Chemical Structure

7-Amino-4-(trifluoromethyl)coumarin Chemical Structure

CAS No. : 53518-15-3

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Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

7-Amino-4-(trifluoromethyl)coumarin (Coumarin 151) is a fluorescent marker for the sensitive detection of proteinases. The excitation and emission wavelengths are 400 and 490 nm, respectively.

In Vitro

Amino acid and peptide derivatives of 7-amino-4-(trifluoromethyl)coumarin are used to monitor peptidase activities. Caspase activation is measured using the fluorogenic compound N-acetyl-asp-glu-val-asp-7-Amino-4-(trifluoromethyl)coumarin (Ac-DEVD-AFC). This substrate rapidly enters cells where it is efficiently cleaved at the aspartate residue by specific caspases, yielding the fluorescent compound 7-Amino-4-(trifluoromethyl)coumarin (AFC). Following cell disruption, released 7-Amino-4-(trifluoromethyl)coumarin is separated on HPLC and detected by fluorescence. The appearance of 7-Amino-4-(trifluoromethyl)coumarin in cells is blocked by the pancaspase inhibitor benzyloxycarbonyl-val-ala-asp-fluoromethylketone, thus establishing that intracellular caspases are responsible for the cleavage[1]. γ-glutamyl-7-amino-4-(trifluoro-methyl)coumarin is synthesized and used as a substrate for fluirimetric assay of γ-gluta-myltranspeptidase. The reaction product 7-amino-4-(trifluoromethyl)coumarin is fluorescent at neutral pH values and with excitation and emission wavelengths of 400 and 490 nm, respectively, concentration is linearly related to fluorescence over the range of 10 to 300 pmol/3 mL reaction mixture[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

229.16

Formula

C₁₀H₆F₃NO₂

CAS No.
SMILES

O=C1C=C(C(F)(F)F)C2=C(O1)C=C(N)C=C2

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

References

Purity: 98.92%

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Keywords:

7-Amino-4-(trifluoromethyl)coumarinCoumarin 151 AFCCoumarin151Coumarin-151OthersInhibitorinhibitorinhibit

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7-Amino-4-(trifluoromethyl)coumarin
Cat. No.:
HY-D0981
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