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  4. 7-Amino-4-(trifluoromethyl)coumarin

7-Amino-4-(trifluoromethyl)coumarin  (Synonyms: Coumarin 151; AFC)

Cat. No.: HY-D0981 Purity: 99.04%
COA
SDS
Handling Instructions Technical Support

7-Amino-4-(trifluoromethyl)coumarin (Coumarin 151) is a fluorescent marker for the sensitive detection of proteinases. The excitation and emission wavelengths are 400 and 490 nm, respectively.

For research use only. We do not sell to patients.

7-Amino-4-(trifluoromethyl)coumarin

7-Amino-4-(trifluoromethyl)coumarin Chemical Structure

CAS No. : 53518-15-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
500 mg In-stock
1 g In-stock
5 g In-stock
10 g In-stock
50 g   Get quote  

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Customer Review

Based on 1 publication(s) in Google Scholar

7-Amino-4-(trifluoromethyl)coumarin Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE 7-Amino-4-(trifluoromethyl)coumarin

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

7-Amino-4-(trifluoromethyl)coumarin (Coumarin 151) is a fluorescent marker for the sensitive detection of proteinases. The excitation and emission wavelengths are 400 and 490 nm, respectively.

Cellular Effect
Cell Line Type Value Description References
SK-MEL-28 IC50
0.98 μM
Compound: 11
Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
[PMID: 37197457]
SK-MEL3 IC50
0.93 μM
Compound: 11
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay
[PMID: 37197457]
In Vitro

Amino acid and peptide derivatives of 7-Amino-4-(trifluoromethyl)coumarin are used to monitor peptidase activities[1].
7-Amino-4-(trifluoromethyl)coumarin fluoresces at neutral pH with excitation and emission wavelengths of 400 nm and 500 nm, respectively. The concentration and fluorescence show a linear relationship in the range of 10 to 300 pmol/3 mL of reaction mixture[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7-Amino-4-(trifluoromethyl)coumarin Related Antibodies
Molecular Weight

229.16

Formula

C10H6F3NO2
CAS No.
Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

454
Excitation (Ex)

344
SMILES

O=C1C=C(C(F)(F)F)C2=C(O1)C=C(N)C=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (436.38 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.3638 mL 21.8188 mL 43.6376 mL
5 mM 0.8728 mL 4.3638 mL 8.7275 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.30%

SDS (393 KB)

COA (249 KB) HNMR (130 KB) RP-HPLC (233 KB) LCMS (96 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 4.3638 mL 21.8188 mL 43.6376 mL 109.0941 mL
5 mM 0.8728 mL 4.3638 mL 8.7275 mL 21.8188 mL
10 mM 0.4364 mL 2.1819 mL 4.3638 mL 10.9094 mL
15 mM 0.2909 mL 1.4546 mL 2.9092 mL 7.2729 mL
20 mM 0.2182 mL 1.0909 mL 2.1819 mL 5.4547 mL
25 mM 0.1746 mL 0.8728 mL 1.7455 mL 4.3638 mL
30 mM 0.1455 mL 0.7273 mL 1.4546 mL 3.6365 mL
40 mM 0.1091 mL 0.5455 mL 1.0909 mL 2.7274 mL
50 mM 0.0873 mL 0.4364 mL 0.8728 mL 2.1819 mL
60 mM 0.0727 mL 0.3636 mL 0.7273 mL 1.8182 mL
80 mM 0.0545 mL 0.2727 mL 0.5455 mL 1.3637 mL
100 mM 0.0436 mL 0.2182 mL 0.4364 mL 1.0909 mL
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7-Amino-4-(trifluoromethyl)coumarin Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Amino-4-(trifluoromethyl)coumarin53518-15-3Coumarin 151 AFCCoumarin151Coumarin-151Fluorescent DyeInhibitorinhibitorinhibit

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