1. JAK/STAT Signaling
    Stem Cell/Wnt
  2. STAT
  3. Cucurbitacin I

Cucurbitacin I (Synonyms: Elatericin B; JSI-124; NSC-521777)

Cat. No.: HY-N1405 Purity: >98.0%
Handling Instructions

Cucurbitacin I is a natural selective inhibitor of JAK2/STAT3, with potent anti-cancer activity.

For research use only. We do not sell to patients.

Cucurbitacin I Chemical Structure

Cucurbitacin I Chemical Structure

CAS No. : 2222-07-3

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 924 In-stock
Estimated Time of Arrival: December 31
1 mg USD 198 In-stock
Estimated Time of Arrival: December 31
5 mg USD 816 In-stock
Estimated Time of Arrival: December 31
10 mg USD 1440 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 2 publication(s) in Google Scholar

Other Forms of Cucurbitacin I:

Top Publications Citing Use of Products

Publications Citing Use of MCE Cucurbitacin I

    Cucurbitacin I purchased from MCE. Usage Cited in: Cell Cycle. 2019 Sep 18:1-20.

    Western blot analysis of C3, NICD, p-Stat3, and Stat3 expression in astrocytes treated with MCM or MCM plus the Stat3 inhibitor JSI-124 (0.2 or 0.5 μM) for 24 h.

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    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review


    Cucurbitacin I is a natural selective inhibitor of JAK2/STAT3, with potent anti-cancer activity.

    IC50 & Target





    In Vitro

    Exposure of the COLO205 cells to Cucurbitacin I significantly decreases cell viability. The anticancer activity of Cucurbitacin I is accomplished by downregulating p-STAT3 and MMP-9 expression[1]. PE-induced cell enlargement and upregulation of ANF and β-MHC are significantly suppressed by pretreatment of the cardiomyocytes with Cucurbitacin I. Notably, Cucurbitacin I also impaires connective tissue growth factor (CTGF) and MAPK signaling, pro-hypertrophic factors, as well as TGF-β/Smad signaling, the important contributing factors to fibrosis[2]. Incubation of the Seax cell line with the Jak/Stat3 inhibitor Cucurbitacin I result in a time- and concentration-dependent decrease of P-Stat3 and Stat3. In freshly isolated Sz cells (n=3), Cucurbitacin I induces a concentration-dependent decrease in Stat3 expression whereas P-Stat3 is undetectable. Finally, incubation of freshly isolated Sz cells (n=4) with 30 μM Cucurbitacin I for 6 hours induces apoptosis in the large majority (73-91%) of tumor cells[3].

    In Vivo

    No major side effects are noted throughout the study. It is shown that average tumor volumes at the end of the study are as follows: control, 616 mm3 (±130); CQ, 580 mm3 (±107); Cucurbitacin I, 346mm3 (±79); and combination, 220mm3 (±62). The differences in tumor volume between the Cucurbitacin I and control, combination and control, and combination and Cucurbitacin I arms are significant. Furthermore, combination-treated tumors exhibit a significantly lower average tumor weight at study termination than the control. Moreover, there was no effect on the body weights of mice[4].

    Molecular Weight




    CAS No.



    CC(C)(C1=CC[[email protected]@]2([H])[[email protected]@]3(C[[email protected]@H](O)[[email protected]@H]([[email protected]]3(C4)C)[[email protected]@](C)(O)C(/C=C/C(C)(O)C)=O)C)C(C(O)=C[[email protected]@]1([H])[[email protected]]2(C)C4=O)=O


    Room temperature in continental US; may vary elsewhere.

    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (194.31 mM)

    *"≥" means soluble, but saturation unknown.

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9431 mL 9.7153 mL 19.4307 mL
    5 mM 0.3886 mL 1.9431 mL 3.8861 mL
    10 mM 0.1943 mL 0.9715 mL 1.9431 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 3 mg/mL (5.83 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 3 mg/mL (5.83 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 3 mg/mL (5.83 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    Animal Administration

    BALB/c nude (nu/nu) female mice are used. U251 cells (5×106 cells in 50 μL of serum-free DMEM) are inoculated subcutaneously into the right flank of 5-week-old female mice after acclimatization for a week. Tumor growth is measured daily with calipers. When the tumors reach a mean volume of 90-120 mm3, animals are randomized into groups. In the first experiment, 16 mice are randomly assigned to Cucurbitacin I (1 mg/kg/day in 20% DMSO in PBS) or drug vehicle control (20% DMSO in PBS) and dosed intraperitoneally with 100 μL of vehicle or drug once daily for 18 days, whereas, in the second, 20 mice are assigned to four groups. Control animals receive 20% DMSO in PBS vehicle, whereas treated animals are injected with Cucurbitacin I (1 mg/kg/day) in 20% DMSO in PBS, CQ (25 mg/kg/day) in 20% DMSO in PBS, and Cucurbitacin I (1 mg/kg/day) plus CQ (25 mg/kg/day) in 20% DMSO in PBS and dosed intraperitoneally with 100 μL of vehicle or drug once daily for 15 days[4].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

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    Cucurbitacin IElatericin BJSI-124NSC-521777JSI124JSI 124NSC521777NSC 521777STATJAKJanus kinaseInhibitorinhibitorinhibit

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