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  3. Cucurbitacin B

Cucurbitacin B 

Cat. No.: HY-N0416 Purity: 99.91%
COA Handling Instructions

Cucurbitacin B belongs to a class of highly oxidized tetracyclic triterpenoids and is oral active. Cucurbitacin B inhibits tumor cell growth, migration and invasion and cycle arrest, but induces cell apoptosis. Cucurbitacin B has potent anti-inflammatory, antioxidant, antiviral, hypoglycemic, hepatoprotective, neuroprotective activity.

For research use only. We do not sell to patients.

Cucurbitacin B Chemical Structure

Cucurbitacin B Chemical Structure

CAS No. : 6199-67-3

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 147 In-stock
Solution
10 mM * 1 mL in DMSO USD 147 In-stock
Solid
1 mg USD 57 In-stock
5 mg USD 120 In-stock
10 mg USD 204 In-stock
25 mg USD 408 In-stock
50 mg USD 720 In-stock
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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of Cucurbitacin B:

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

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  • Customer Review

Description

Cucurbitacin B belongs to a class of highly oxidized tetracyclic triterpenoids and is oral active. Cucurbitacin B inhibits tumor cell growth, migration and invasion and cycle arrest, but induces cell apoptosis. Cucurbitacin B has potent anti-inflammatory, antioxidant, antiviral, hypoglycemic, hepatoprotective, neuroprotective activity[1][2][3][4][5].

In Vitro

Cucurbitacin B (up to 40 μM, 12-48 h) inhibits the cell growth and arrests cell cycle progression at the G2/M phase in CCA cell lines [2].
Cucurbitacin B (0.1, 0.3 and 1 μM) increases the level of total superoxide dismutase(T-SOD) and SOD-1, but decrease the reactive oxygen species (ROS) and malondialdehyde (MDA) in BY4741 yeast cells[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: CCA cell lines
Concentration: 0.1, 1 0.5, 1,5, 10, 20, 40 μM
Incubation Time: 24 and 48 h
Result: Decreased cell viability in a dose-dependent and time-dependent manner with the IC50 values of 13:44 μM for 24 h and 1.55 for 48 h.

Cell Cycle Analysis[2]

Cell Line: CCA cell lines
Concentration: 0.1, 1, 10 μM
Incubation Time: 24 h
Result: Arrested cell cycle progression at the G2/M phase.

Western Blot Analysis[2]

Cell Line: CCA cell lines
Concentration: 0.1, 1 0.5, 1,5, 10, 20, 40 μM
Incubation Time: 12 and 24 h
Result: Decreased the expression of Cyclin A, Cyclin D1, Cdc25A, but increased the level of p21.
In Vivo

Cucurbitacin B (5 mg/kg, oral administration 10day) B shows protective effects against carrageenan-induced prostatitis in rats[4].
Cucurbitacin B (20-50 mg/kg for i.p., 28 day) B afford a decline in AD symptoms and protects neurons against STZ-ICV toxicity that improved memory functions in rats[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Carrageenan-induced prostatic inflammation in rats[4]
Dosage: 5mg/kg/day for 10day
Administration: Oral administration
Result: Decreased the level of TNF-α, IL-1b, COX-2 and iNOS in prostatic tissues.
Animal Model: STZ-ICV rat prototype of AD-like dementia[5]
Dosage: 20, 50mg/kg/day for 28days
Administration: Intraperitoneal injection (i.p.)
Result: Decreased the TNF-α, IL-1β, MPO, iNOS, acetylcholinesterase, and glutamate levels, but increased gamma-aminobutyric acid.
Increase in viable neuron density in the cortex and hippocampus of rats.
Molecular Weight

558.70

Formula

C32H46O8

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O[C@H](C1)[C@@]([C@@](C)(O)C(/C=C/C(OC(C)=O)(C)C)=O)([H])[C@](C2)(C)[C@]1(C)[C@]3([H])CC=C4C(C)(C)C([C@@H](O)C[C@@]4([H])[C@]3(C)C2=O)=O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (178.99 mM)

H2O : 1 mg/mL (1.79 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7899 mL 8.9493 mL 17.8987 mL
5 mM 0.3580 mL 1.7899 mL 3.5797 mL
10 mM 0.1790 mL 0.8949 mL 1.7899 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.47 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (4.47 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.91%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cucurbitacin B
Cat. No.:
HY-N0416
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