1. Anti-infection
    Cell Cycle/DNA Damage
    Apoptosis
  2. Influenza Virus
    DNA/RNA Synthesis
    Apoptosis
    Parasite
    Bacterial
    Fungal
  3. Cynaroside

Cynaroside  (Synonyms: Luteolin 7-glucoside; Luteolin 7-O-β-D-glucoside)

Cat. No.: HY-N0540 Purity: 99.19%
COA Handling Instructions

Cynaroside (Luteolin 7-glucoside) is a flavonoid compound that exhibits anti-oxidative capabilities. Cynaroside is also a potent influenza RNA-dependent RNA polymerase inhibitor with an IC50 of 32 nM. Cynaroside also is a promising inhibitor for H2O2-induced apoptosis, has cytoprotection against oxidative stress-induced cardiovascular diseases. Cynaroside also has antibacterial, antifungal and anticancer activities, antioxidant and anti-inflammatory activities.

For research use only. We do not sell to patients.

Cynaroside Chemical Structure

Cynaroside Chemical Structure

CAS No. : 5373-11-5

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10 mM * 1 mL in DMSO USD 106 In-stock
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25 mg USD 288 In-stock
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Customer Review

Based on 6 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cynaroside (Luteolin 7-glucoside) is a flavonoid compound that exhibits anti-oxidative capabilities. Cynaroside is also a potent influenza RNA-dependent RNA polymerase inhibitor with an IC50 of 32 nM. Cynaroside also is a promising inhibitor for H2O2-induced apoptosis, has cytoprotection against oxidative stress-induced cardiovascular diseases. Cynaroside also has antibacterial, antifungal and anticancer activities, antioxidant and anti-inflammatory activities[1][3][4][5].

IC50 & Target

IC50:32 nM (RNA polymerase inhibitor)[2]

In Vitro

Cynaroside promots macrophage phenotypic transition from pro-inflammatory M1 to anti-inflammatory M2, and mitigates sepsis-associated liver inflammatory damage.
Cynaroside reduces binding of PKM2 to hypoxia-inducible factor-1α (HIF-1α) by abolishing translocation of PKM2 to the nucleus and promoting PKM2 tetramer formation, as well as suppressing phosphorylation of PKM2 at Y105 in vivo and in vitro.
Cynaroside restores pyruvate kinase activity, inhibits glycolysis-related proteins including PFKFB3, HK2 and HIF-1α, and inhibits glycolysisrelated hyperacetylation of HMGB1 in septic liver.
Cynaroside protects H9c2 cells against H2O2-induced apoptosis by decreasing ROS generation and inhibiting caspase activation in both the mitochondrial and death receptor pathways.
Cynaroside maintains mitochondrial function by regulating Bcl-2 protein expression, as well as JNK and P53 expression[2][3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2]

Cell Line: RAW264.7 cell
Concentration: 5µM, 10µM
Incubation Time: 2 h
Result: Reduced expression levels of IL-1β, IL-6 and TNF-α and HMGB1, suppressed M1 polarized phenotype in RAW264.7 cells.

Immunofluorescence[2]

Cell Line: RAW264.7 cell
Concentration:
Incubation Time: 4 h
Result: Decreased PKM2 nuclear translocation in hepatic macrophages of septic mice.

RT-PCR[2]

Cell Line: RAW264.7 cell
Concentration: 2.5µM, 5µM, 10µM
Incubation Time:
Result: Increased increased expression levels of M2 markers Arg-1, IL-10 andCD206.

Cell Viability Assay[3]

Cell Line: H9c2 cells
Concentration: 25, 50, 100 μg/mL
Incubation Time: 4 h
Result: Protected H9c2 cells from oxidative stress-induced cell injury.

Apoptosis Analysis[3]

Cell Line: H9c2 cells
Concentration: 25, 50, 100 μg/mL
Incubation Time: 4 h
Result: Decreased H2O2-induced apoptosis in H9C2 cells.
In Vivo

Cynaroside (i.p.; 5mg/kg) reduces binding of PKM2 to hypoxia-inducible factor-1α (HIF-1α) by abolishing translocation of PKM2 to the nucleus and promoting PKM2 tetramer formation, as well as suppressing phosphorylation of PKM2 at Y105[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice model of sepsis[2]
Dosage: 5mg/kg
Administration: Cynaroside (i.p.; 5mg/kg)
Result: Inhibited PKM2 dimer formation in liver of septic mice.
Molecular Weight

448.38

Formula

C21H20O11

CAS No.
SMILES

O=C(C=C(C1=CC(O)=C(O)C=C1)OC2=CC(O[[email protected]@H]([[email protected]@H]([[email protected]@H](O)[[email protected]@H]3O)O)O[[email protected]@H]3CO)=C4)C2=C4O

Structure Classification
Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (185.85 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2303 mL 11.1513 mL 22.3025 mL
5 mM 0.4461 mL 2.2303 mL 4.4605 mL
10 mM 0.2230 mL 1.1151 mL 2.2303 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  50% PEG300    50% saline

    Solubility: 16.67 mg/mL (37.18 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.64 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.64 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.19%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cynaroside
Cat. No.:
HY-N0540
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