1. Anti-infection Cell Cycle/DNA Damage Apoptosis
  2. Influenza Virus DNA/RNA Synthesis Apoptosis Parasite Bacterial Fungal
  3. Cynaroside

Cynaroside  (Synonyms: Luteolin 7-glucoside; Luteolin 7-O-β-D-glucoside)

Cat. No.: HY-N0540 Purity: 99.19%
COA Handling Instructions

Cynaroside (Luteolin 7-glucoside) is a flavonoid compound that exhibits anti-oxidative capabilities. Cynaroside is also a potent influenza RNA-dependent RNA polymerase inhibitor with an IC50 of 32 nM. Cynaroside also is a promising inhibitor for H2O2-induced apoptosis, has cytoprotection against oxidative stress-induced cardiovascular diseases. Cynaroside also has antibacterial, antifungal and anticancer activities, antioxidant and anti-inflammatory activities.

For research use only. We do not sell to patients.

Cynaroside Chemical Structure

Cynaroside Chemical Structure

CAS No. : 5373-11-5

Size Price Stock Quantity
Solid + Solvent
10 mM * 1 mL in DMSO
ready for reconstitution
USD 99 In-stock
Solution
10 mM * 1 mL in DMSO USD 99 In-stock
Solid
5 mg USD 90 In-stock
10 mg USD 140 In-stock
25 mg USD 280 In-stock
50 mg USD 450 In-stock
100 mg USD 720 In-stock
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500 mg   Get quote  

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Customer Review

Based on 6 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Cynaroside (Luteolin 7-glucoside) is a flavonoid compound that exhibits anti-oxidative capabilities. Cynaroside is also a potent influenza RNA-dependent RNA polymerase inhibitor with an IC50 of 32 nM. Cynaroside also is a promising inhibitor for H2O2-induced apoptosis, has cytoprotection against oxidative stress-induced cardiovascular diseases. Cynaroside also has antibacterial, antifungal and anticancer activities, antioxidant and anti-inflammatory activities[1][3][4][5].

IC50 & Target

IC50:32 nM (RNA polymerase inhibitor)[2]

In Vitro

Cynaroside promots macrophage phenotypic transition from pro-inflammatory M1 to anti-inflammatory M2, and mitigates sepsis-associated liver inflammatory damage.
? Cynaroside reduces binding of PKM2 to hypoxia-inducible factor-1α (HIF-1α) by abolishing translocation of PKM2 to the nucleus and promoting PKM2 tetramer formation, as well as suppressing phosphorylation of PKM2 at Y105 in vivo and in vitro.
? Cynaroside restores pyruvate kinase activity, inhibits glycolysis-related proteins including PFKFB3, HK2 and HIF-1α, and inhibits glycolysisrelated hyperacetylation of HMGB1 in septic liver.
? Cynaroside protects H9c2 cells against H2O2-induced apoptosis by decreasing ROS generation and inhibiting caspase activation in both the mitochondrial and death receptor pathways.
? Cynaroside maintains mitochondrial function by regulating Bcl-2 protein expression, as well as JNK and P53 expression[2][3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[2]

Cell Line: RAW264.7 cell
Concentration: 5µM, 10µM
Incubation Time: 2 h
Result: Reduced expression levels of IL-1β, IL-6 and TNF-α and HMGB1, suppressed M1 polarized phenotype in RAW264.7 cells.

Immunofluorescence[2]

Cell Line: RAW264.7 cell
Concentration:
Incubation Time: 4 h
Result: Decreased PKM2 nuclear translocation in hepatic macrophages of septic mice.

RT-PCR[2]

Cell Line: RAW264.7 cell
Concentration: 2.5µM, 5µM, 10µM
Incubation Time:
Result: Increased increased expression levels of M2 markers Arg-1, IL-10 andCD206.

Cell Viability Assay[3]

Cell Line: H9c2 cells
Concentration: 25, 50, 100 μg/mL
Incubation Time: 4 h
Result: Protected H9c2 cells from oxidative stress-induced cell injury.

Apoptosis Analysis[3]

Cell Line: H9c2 cells
Concentration: 25, 50, 100 μg/mL
Incubation Time: 4 h
Result: Decreased H2O2-induced apoptosis in H9C2 cells.
In Vivo

Cynaroside (i.p.; 5mg/kg) reduces binding of PKM2 to hypoxia-inducible factor-1α (HIF-1α) by abolishing translocation of PKM2 to the nucleus and promoting PKM2 tetramer formation, as well as suppressing phosphorylation of PKM2 at Y105[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice model of sepsis[2]
Dosage: 5mg/kg
Administration: Cynaroside (i.p.; 5mg/kg)
Result: Inhibited PKM2 dimer formation in liver of septic mice.
Molecular Weight

448.38

Formula

C21H20O11

CAS No.
Appearance

Solid

SMILES

O=C(C=C(C1=CC(O)=C(O)C=C1)OC2=CC(O[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO)=C4)C2=C4O

Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 83.33 mg/mL (185.85 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.2303 mL 11.1513 mL 22.3025 mL
5 mM 0.4461 mL 2.2303 mL 4.4605 mL
10 mM 0.2230 mL 1.1151 mL 2.2303 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  50% PEG300    50% Saline

    Solubility: 16.67 mg/mL (37.18 mM); Suspended solution; Need ultrasonic

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.08 mg/mL (4.64 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.08 mg/mL (4.64 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).
Purity & Documentation

Purity: 99.19%

References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cynaroside
Cat. No.:
HY-N0540
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