1. Metabolic Enzyme/Protease
  2. MMP
  3. Ilomastat

Ilomastat (Synonyms: GM6001; Galardin)

Cat. No.: HY-15768 Purity: 98.48%
Handling Instructions

Ilomastat (GM6001) is a potent and broad spectrum matrix metalloprotease (MMP) inhibitor, inhibits MMPs (IC50s, 1.5 nM for MMP-1; 1.1 nM for MMP-2; 1.9 nM for MMP-3; 0.5 nM for MMP-9), with a Ki of 0.4 nM for human skin fibroblast collagenase (MMP-1).

For research use only. We do not sell to patients.

Ilomastat Chemical Structure

Ilomastat Chemical Structure

CAS No. : 142880-36-2

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 119 In-stock
Estimated Time of Arrival: December 31
5 mg USD 108 In-stock
Estimated Time of Arrival: December 31
10 mg USD 180 In-stock
Estimated Time of Arrival: December 31
50 mg USD 540 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
200 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 16 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Ilomastat purchased from MCE. Usage Cited in: Biochem Biophys Res Commun. 2018 Mar 25;498(1):125-131.

    Cell lysates are immunoblotted with antibodies against Claudin-4, E-cadherin, β-catenin, MMP9 and GAPDH.

    Ilomastat purchased from MCE. Usage Cited in: Nat Commun. 2017 Mar 7;8:14483.

    A transwell assay is performed in MDA-MB-231 cells, GM6001-treated MDA-MB-231 cells or GM6001-treated and CRISPR/Cas9-mediated VCL deleted MDA-MB-231 cells

    Ilomastat purchased from MCE. Usage Cited in: Int J Nanomedicine. 2018 Aug 13;13:4641-4659.

    Cellular uptake of P123-Cou6 and PG-SG-Cou6 micelles in MCF7 and MCF7/ADR cells in the presence of GM6001.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ilomastat (GM6001) is a potent and broad spectrum matrix metalloprotease (MMP) inhibitor, inhibits MMPs (IC50s, 1.5 nM for MMP-1; 1.1 nM for MMP-2; 1.9 nM for MMP-3; 0.5 nM for MMP-9), with a Ki of 0.4 nM for human skin fibroblast collagenase (MMP-1).

    IC50 & Target[1][5]

    MMP-9

    0.5 nM (IC50)

    MMP-2

    1.1 nM (IC50)

    MMP-1

    1.5 nM (IC50)

    MMP-3

    1.9 nM (IC50)

    Fibroblast collagenase

    0.4 nM (Ki, Human skin)

    Thermolysin

    20 nM (Ki)

    Eastase

    20 nM (Ki)

    In Vitro

    Ilomastat (GM6001) inhibits human skin fibroblast collagenase, thermolysin and elastase with Kis of 0.4 nM, 20 nM, 20 nM, resepctively[1]. Ilomastat (0.1-10 nM) inhibits gelatinase A and gelatinase B produced by T-cells. Ilomastat inhibits T-cell homing[4].

    In Vivo

    Ilomastat (GM6001) (400 μg/mL) inhibits corneal ulceration after severe alkali injury in animals[2]. Ilomastat (GM6001) significantly suppresses intimal hyperplasia and intimalcollagen content. Ilomastat increases lumen area in stented arteries, shows no activity on proliferation rates in rabbit model after stenting[3].

    Molecular Weight

    388.46

    Formula

    C₂₀H₂₈N₄O₄

    CAS No.

    142880-36-2

    SMILES

    O=C(N[[email protected]@H](CC1=CNC2=C1C=CC=C2)C(NC)=O)[[email protected]](CC(C)C)CC(NO)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 47 mg/mL (120.99 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5743 mL 12.8713 mL 25.7427 mL
    5 mM 0.5149 mL 2.5743 mL 5.1485 mL
    10 mM 0.2574 mL 1.2871 mL 2.5743 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (6.44 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.5 mg/mL (6.44 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (6.44 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Animal Administration
    [3]

    To assess the effects of MMP inhibition, animals are given daily injections of either vehicle (“placebo group”) or Ilomastat (GM6001) (100 mg/kg per day as subcutaneous suspension), beginning one day before the second injury until seven days after the procedure. Ilomastat (GM6001) is a nonspecific hydroxamic acid-based MMPI with potent inhibitory activity against collagenase, gelatinases and stromelysin. Animals are euthanized at either 1 week or 10 weeks after the second injury.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 98.48%

    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.
    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Keywords:

    IlomastatGM6001GalardinGM 6001GM-6001MMPMatrix metalloproteinasesInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product name

     

    Salutation

    Applicant name *

     

    Email address *

    Phone number *

     

    Organization name *

    Country or Region *

     

    Requested quantity *

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product name:
    Ilomastat
    Cat. No.:
    HY-15768
    Quantity:
    MCE Japan Authorized Agent: