HC-070
Based on 7 publication(s) in Google Scholar
HC-070 is an antagonist of TRPC4/TRPC5, with IC50s of 9.3 nM and 46 nM for hTRPC5 and hTRPC4 in cells, respectively.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 1628291-95-1
- Formula: C22H20Cl2N4O4
- Molecular Weight:475.32
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) HC-070
More- Sci Transl Med. 2021 May 26;13(595):eabd7702. [Abstract]
- Pharmacol Res. 2025 Feb 7:107648. [Abstract]
- EMBO J. 2024 Dec;43(23):5813-5836. [Abstract]
- EMBO Mol Med. 2024 Aug;16(8):1817-1839. [Abstract]
- Free Radic Biol Med. 2026 Mar 13:250:30-48. [Abstract]
- Neurochem Int. 2023 Nov:170:105609. [Abstract]
- bioRxiv. 2020 Jul.
Biological Activity
IC50: 9.3 nM (hTRPC5, cell assay), 46 nM (hTRPC4, cell assay)[1]
HC-070 is an antagonist of TRPC4/TRPC5, with IC50s of 9.3 nM and 46 nM for hTRPC5 and hTRPC4, respectively. HC-070 weakly inhibits TRPC3 (IC50, 1 μM), and is at least 400-fold selective for human TRPC4 and TRPC5-containing channels versus the other channels examined. HC-070 inhibits lanthanum-activated hTRPC5-, mTRPC5-, rTRPC5-mediated currents with IC50s of 0.52 nM, 0.55 nM, and 0.32 nM in whole-cell manual patch clamp. Furthermore, HC-070 blocks M2R-activated human TRPC1/TRPC4 channels with an IC50 of 1.3 nM and La3+- and M1R-activated human TRPC1/5 channels with IC50s of 1.4 nM and 4.4 nM. HC-070 inhibits human TRPC5 currents activated via muscarinic type 1 (M1R) with an IC50 of 2.0 nM. HC-070 also suppresses hTRPC4 currents via M2R with an IC50 of 0.49 nM. HC-070 (20 nM) reduces CCK-4 evoked neuronal activity in the amygdala slices[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 1628291-95-1
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Appearance Solid
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Molecular Weight 475.32
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Formula C22H20Cl2N4O4
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Color White to off-white
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SMILES
O=C(N1CCCO)N(C)C2=C(N(CC3=CC=C(Cl)C=C3)C(OC4=CC=CC(Cl)=C4)=N2)C1=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (7)
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Journal Impact Factor
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Most Recent
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Sci Transl Med
Transient receptor potential canonical 5 mediates inflammatory mechanical and spontaneous pain in mice. [Abstract]2021 May 26;13(595):eabd7702. PMID: 34039739 -
Pharmacol Res
The essential role of sphingolipids in TRPC5 ion channel localization and functionality within lipid rafts. [Abstract]2025 Feb 7:107648. PMID: 39923924 -
EMBO J
2024 Dec;43(23):5813-5836. PMID: 39375537 -
EMBO Mol Med
In vitro and in vivo inhibition of the host TRPC4 channel attenuates Zika virus infection. [Abstract]2024 Aug;16(8):1817-1839. PMID: 39009885 -
Free Radic Biol Med
ICAM-1 targeted extracellular vesicles loaded with HC-070 mitigate vascular oxidative stress and inflammation in obstructive sleep apnea hypopnea syndrome by inhibiting TRPC5. [Abstract]2026 Mar 13:250:30-48. PMID: 41833586 -
Neurochem Int
BTD: A TRPC5 activatorameliorates mechanical allodynia in diabetic peripheral neuropathic rats by modulating TRPC5-CAMKII-ERK pathway. [Abstract]2023 Nov:170:105609. PMID: 37673218 -
Solvent & Solubility
DMSO : ≥ 62.5 mg/mL (131.49 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.26 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Mice[1]
The room is illuminated with fluorescent lighting on a 12-hour light/dark cycle. The light cycle is reversed, so that the dark cycle is from 6 am-6 pm daily and studies are performed when animals are more active. Groups of male C57/BL6 mice (10 weeks old) are dosed PO with 0.5% methyl cellulose or HC-070 at 0.3, 1 or 3 mg/kg (n = 10). The positive control, 1.5 mg/kg diazepam, is administered IP 30 minutes prior to testing (n = 10). Immediately following dosing, mice are returned to their home cage. At 60 minutes post vehicle or HC-070 administration, and 30 minutes post diazepam administration, mice are placed onto the elevated plus maze, one at a time, and their session recorded for 5 minutes. Videos are manually scored for number of open arm entries by a scorer blinded to treatment. All animals that fall off the maze during the test are removed from analysis[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1038 mL | 10.5192 mL | 21.0385 mL | 52.5961 mL |
| 5 mM | 0.4208 mL | 2.1038 mL | 4.2077 mL | 10.5192 mL | |
| 10 mM | 0.2104 mL | 1.0519 mL | 2.1038 mL | 5.2596 mL | |
| 15 mM | 0.1403 mL | 0.7013 mL | 1.4026 mL | 3.5064 mL | |
| 20 mM | 0.1052 mL | 0.5260 mL | 1.0519 mL | 2.6298 mL | |
| 25 mM | 0.0842 mL | 0.4208 mL | 0.8415 mL | 2.1038 mL | |
| 30 mM | 0.0701 mL | 0.3506 mL | 0.7013 mL | 1.7532 mL | |
| 40 mM | 0.0526 mL | 0.2630 mL | 0.5260 mL | 1.3149 mL | |
| 50 mM | 0.0421 mL | 0.2104 mL | 0.4208 mL | 1.0519 mL | |
| 60 mM | 0.0351 mL | 0.1753 mL | 0.3506 mL | 0.8766 mL | |
| 80 mM | 0.0263 mL | 0.1315 mL | 0.2630 mL | 0.6575 mL | |
| 100 mM | 0.0210 mL | 0.1052 mL | 0.2104 mL | 0.5260 mL |
HC-070 Related Classifications
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