2. Membrane Transporter/Ion Channel Neuronal Signaling
  3. TRP Channel
  4. Pyr3

Pyr3 

Cat. No.: HY-108465 Purity: 99.90%
COA Handling Instructions

Pyr3 is a selective inhibitor of transient receptor potential canonical channel 3 (TRPC3), with an IC50 of 700 nM for TRPC3-mediated Ca2+ influx.

For research use only. We do not sell to patients.

Pyr3 Chemical Structure

Pyr3 Chemical Structure

CAS No. : 1160514-60-2

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 97 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 97 In-stock
Solid
5 mg USD 88 In-stock
10 mg USD 132 In-stock
25 mg USD 275 In-stock
50 mg USD 462 In-stock
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Customer Review

Based on 5 publication(s) in Google Scholar

Pyr3 Related Antibodies

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Description

Pyr3 is a selective inhibitor of transient receptor potential canonical channel 3 (TRPC3), with an IC50 of 700 nM for TRPC3-mediated Ca2+ influx.

IC50 & Target

IC50: 700 nM (TRPC3)[1].
In Vitro

Pyr3 selectively and directly inhibits TRPC3 channels among TRPC family members. Application of Pyr3 inhibits TRPC3-mediated Ca2+ influx in a dose-dependent manner with the IC50 value of 700 nM. Pyr3 becomes apparent at 0.3 μM, and is almost complete at 3 μM. Interestingly, Ca2+ influx is inhibited by Pyr3 in cells co-expressing TRPC3 plus TRPC6 but not in cells coexpressing TRPC1 plus TRPC5. The Ang II-induced NFAT translocation is suppressed by Pyr3, but weakly by Pyr2 in a concentration-dependent manner (IC50 value was 0.05 μM for Pyr3 and 2 μM for Pyr2)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Pyr3 Related Antibodies
Molecular Weight

456.63

Appearance

Solid

Formula

C16H11Cl3F3N3O3
CAS No.
SMILES

O=C(C1=C(C(F)(F)F)N(C2=CC=C(NC(/C(Cl)=C(Cl)/Cl)=O)C=C2)N=C1)OCC
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (273.74 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1900 mL 10.9498 mL 21.8996 mL
5 mM 0.4380 mL 2.1900 mL 4.3799 mL
10 mM 0.2190 mL 1.0950 mL 2.1900 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.56 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.56 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.56 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.90%

COA (248 KB) RP-HPLC (240 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

HEK293 cells or HEK293T cells are used throughout the study. Average time courses of Ca2+ responses induced by 100 μM UTP with Pyr3 at indicated concentrations (0.1, 1, 3, 10 μM) in TRPC-transfected HEK293 cells are tested[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Pyr3 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

Pyr31160514-60-2Pyr 3Pyr-3TRP ChannelTransient receptor potential channelsInhibitorinhibitorinhibit

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