1. Membrane Transporter/Ion Channel
  2. TRP Channel

Pyr3 

Cat. No.: HY-108465 Purity: 99.89%
Handling Instructions

Pyr3 is a selective inhibitor of transient receptor potential canonical channel 3 (TRPC3), with an IC50 of 700 nM for TRPC3-mediated Ca2+ influx.

For research use only. We do not sell to patients.

Pyr3 Chemical Structure

Pyr3 Chemical Structure

CAS No. : 1160514-60-2

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 88 In-stock
Estimated Time of Arrival: December 31
5 mg USD 80 In-stock
Estimated Time of Arrival: December 31
10 mg USD 120 In-stock
Estimated Time of Arrival: December 31
25 mg USD 250 In-stock
Estimated Time of Arrival: December 31
50 mg USD 420 In-stock
Estimated Time of Arrival: December 31
100 mg   Get quote  
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  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Pyr3 is a selective inhibitor of transient receptor potential canonical channel 3 (TRPC3), with an IC50 of 700 nM for TRPC3-mediated Ca2+ influx.

IC50 & Target

IC50: 700 nM (TRPC3)[1].

In Vitro

Pyr3 selectively and directly inhibits TRPC3 channels among TRPC family members. Application of Pyr3 inhibits TRPC3-mediated Ca2+ influx in a dose-dependent manner with the IC50 value of 700 nM. Pyr3 becomes apparent at 0.3 μM, and is almost complete at 3 μM. Interestingly, Ca2+ influx is inhibited by Pyr3 in cells co-expressing TRPC3 plus TRPC6 but not in cells coexpressing TRPC1 plus TRPC5. The Ang II-induced NFAT translocation is suppressed by Pyr3, but weakly by Pyr2 in a concentration-dependent manner (IC50 value was 0.05 μM for Pyr3 and 2 μM for Pyr2)[1].

Solvent & Solubility
In Vitro: 

DMSO : ≥ 125 mg/mL (273.74 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1900 mL 10.9498 mL 21.8996 mL
5 mM 0.4380 mL 2.1900 mL 4.3799 mL
10 mM 0.2190 mL 1.0950 mL 2.1900 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.56 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.56 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.56 mM); Clear solution

References
Cell Assay
[1]

HEK293 cells or HEK293T cells are used throughout the study. Average time courses of Ca2+ responses induced by 100 μM UTP with Pyr3 at indicated concentrations (0.1, 1, 3, 10 μM) in TRPC-transfected HEK293 cells are tested[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

456.63

Formula

C₁₆H₁₁Cl₃F₃N₃O₃

CAS No.

1160514-60-2

SMILES

O=C(C1=C(C(F)(F)F)N(C2=CC=C(NC(/C(Cl)=C(Cl)/Cl)=O)C=C2)N=C1)OCC

Storage
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
Shipping

Room temperature in continental US; may vary elsewhere

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Product Name:
Pyr3
Cat. No.:
HY-108465
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Pyr3

Cat. No.: HY-108465