SKLB-23bb

Name: SKLB-23bb
Brand: MedChemExpress
SKU: HY-18947
Rating: 4.5 (0 reviews)

Cat. No.: HY-18947 Purity: 98.0%: Data Sheet
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SKLB-23bb is a potent and selective inhibitor for HDAC6 with an IC₅₀ of 17 nM and shows 25-fold and 200-fold selectivity relative to HDAC1 (IC₅₀=422 nM) and HDAC8 (IC₅₀=3398 nM), respectively.

For research use only. We do not sell to patients.

SKLB-23bb Chemical Structure

CAS No. : 1815580-06-3

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All HDAC Isoform Specific Products:

View All Isoforms

HDAC HDAC1 HDAC2 HDAC3 HDAC4 HDAC5 HDAC6 HDAC7 HDAC8 HDAC9 HDAC10 HDAC11 HD1 HD2

Biological Activity
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Description

IC₅₀ & Target

IC50: 17 nM (HDAC6), 422 nM (HDAC1), 398 nM (HDAC8)^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
U-266	IC₅₀	14 1 Compound: 23bb	Cytotoxicity against human U266 cells after 72 hrs by MTT assay Cytotoxicity against human U266 cells after 72 hrs by MTT assay	[PMID: 26443078]
A2780	IC₅₀	46 1 Compound: 23bb	Cytotoxicity against human A2780 cells after 72 hrs by MTT assay Cytotoxicity against human A2780 cells after 72 hrs by MTT assay	[PMID: 26443078]
A-375	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human A-375 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human A-375 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
RPMI-8226	IC₅₀	15 1 Compound: 23bb	Cytotoxicity against human RPMI8226 cells after 72 hrs by MTT assay Cytotoxicity against human RPMI8226 cells after 72 hrs by MTT assay	[PMID: 26443078]
A2780S	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human A2780S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human A2780S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
A-375	IC₅₀	45 1 Compound: 23bb	Antiproliferative activity against human A375 cells after 72 hrs by MTT assay Antiproliferative activity against human A375 cells after 72 hrs by MTT assay	[PMID: 26443078]
A-375	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human A-375 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human A-375 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
SK-BR-3	IC₅₀	22 1 Compound: 23bb	Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay	[PMID: 26443078]
A2780	IC₅₀	46 1 Compound: 23bb	Cytotoxicity against human A2780 cells after 72 hrs by MTT assay Cytotoxicity against human A2780 cells after 72 hrs by MTT assay	[PMID: 26443078]
A2780S	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human A2780S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human A2780S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
A-375	IC₅₀	45 1 Compound: 23bb	Antiproliferative activity against human A375 cells after 72 hrs by MTT assay Antiproliferative activity against human A375 cells after 72 hrs by MTT assay	[PMID: 26443078]
A2780S	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human A2780S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human A2780S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
A549	IC₅₀	104 1 Compound: 23bb	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 26443078]
A-375	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human A-375 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human A-375 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
A549	IC₅₀	104 1 Compound: 23bb	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 26443078]
A549	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
A549	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
A549	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human A549 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HBL1	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HBL1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HBL1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HBL1	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HBL1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HBL1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HBL1	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HBL1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HBL1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HCT-116	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HCT-116	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HepG2	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HCT-116	IC₅₀	35 1 Compound: 23bb	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 26443078]
HT-29	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HeLa	EC₅₀	1741 1 Compound: 23bb	Induction of histone H3 acetylation in human HeLa cells incubated for 6 hrs by cytoblot assay Induction of histone H3 acetylation in human HeLa cells incubated for 6 hrs by cytoblot assay	[PMID: 26443078]
JeKo-1	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human JeKo-1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human JeKo-1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HeLa	IC₅₀	49 1 Compound: 23bb	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 26443078]
HeLa	EC₅₀	58.9 1 Compound: 23bb	Inhibition of HDAC6 in human HeLa cells assessed as induction of tubulin acetylation incubated for 6 hrs by cytoblot assay Inhibition of HDAC6 in human HeLa cells assessed as induction of tubulin acetylation incubated for 6 hrs by cytoblot assay	[PMID: 26443078]
K562	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human K562 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HCT-116	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HCT-116 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HCT-116	IC₅₀	35 1 Compound: 23bb	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 26443078]
MCF7	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HepG2	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
MDA-MB-231	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HepG2	IC₅₀	41 1 Compound: 23bb	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 26443078]
HT-29	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HT-29	IC₅₀	72 1 Compound: 23bb	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 26443078]
MM1.S	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MM1.S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MM1.S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HT-29	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HT-29 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HT-29	IC₅₀	72 1 Compound: 23bb	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 26443078]
HeLa	EC₅₀	1741 1 Compound: 23bb	Induction of histone H3 acetylation in human HeLa cells incubated for 6 hrs by cytoblot assay Induction of histone H3 acetylation in human HeLa cells incubated for 6 hrs by cytoblot assay	[PMID: 26443078]
MV4-11	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MV4-11 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MV4-11 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
NCI-H460	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
JeKo-1	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human JeKo-1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human JeKo-1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HeLa	EC₅₀	58.9 1 Compound: 23bb	Inhibition of HDAC6 in human HeLa cells assessed as induction of tubulin acetylation incubated for 6 hrs by cytoblot assay Inhibition of HDAC6 in human HeLa cells assessed as induction of tubulin acetylation incubated for 6 hrs by cytoblot assay	[PMID: 26443078]
Ramos	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human Ramos cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human Ramos cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
K562	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human K562 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HeLa	IC₅₀	49 1 Compound: 23bb	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 26443078]
RPMI-8226	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human RPMI-8226 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human RPMI-8226 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
K562	IC₅₀	63 1 Compound: 23bb	Cytotoxicity against human K562 cells after 72 hrs by MTT assay Cytotoxicity against human K562 cells after 72 hrs by MTT assay	[PMID: 26443078]
HepG2	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human HepG2 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
SK-OV-3	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
MCF7	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HepG2	IC₅₀	41 1 Compound: 23bb	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 26443078]
U-266	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human U-266 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human U-266 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
MDA-MB-231	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
JeKo-1	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human JeKo-1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human JeKo-1 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HCT-116	IC₅₀	35 1 Compound: 23bb	Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells after 72 hrs by MTT assay	[PMID: 26443078]
MM1.S	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MM1.S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MM1.S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
K562	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human K562 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human K562 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
MV4-11	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MV4-11 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MV4-11 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HepG2	IC₅₀	41 1 Compound: 23bb	Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay Cytotoxicity against human HepG2 cells after 72 hrs by MTT assay	[PMID: 26443078]
K562	IC₅₀	63 1 Compound: 23bb	Cytotoxicity against human K562 cells after 72 hrs by MTT assay Cytotoxicity against human K562 cells after 72 hrs by MTT assay	[PMID: 26443078]
MCF7	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
MV4-11	IC₅₀	60 1 Compound: 23bb	Cytotoxicity against human MV4-11 cells after 72 hrs by MTT assay Cytotoxicity against human MV4-11 cells after 72 hrs by MTT assay	[PMID: 26443078]
A-375	IC₅₀	45 1 Compound: 23bb	Antiproliferative activity against human A375 cells after 72 hrs by MTT assay Antiproliferative activity against human A375 cells after 72 hrs by MTT assay	[PMID: 26443078]
NCI-H460	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
A2780	IC₅₀	46 1 Compound: 23bb	Cytotoxicity against human A2780 cells after 72 hrs by MTT assay Cytotoxicity against human A2780 cells after 72 hrs by MTT assay	[PMID: 26443078]
MDA-MB-231	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
MM1.S	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MM1.S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MM1.S cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HeLa	IC₅₀	49 1 Compound: 23bb	Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay Antiproliferative activity against human HeLa cells after 72 hrs by MTT assay	[PMID: 26443078]
NCI-H460	IC₅₀	55 1 Compound: 23bb	Cytotoxicity against human H460 cells after 72 hrs by MTT assay Cytotoxicity against human H460 cells after 72 hrs by MTT assay	[PMID: 26443078]
Ramos	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human Ramos cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human Ramos cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
SK-OV-3	IC₅₀	50 1 Compound: 23bb	Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay	[PMID: 26443078]
Ramos	IC₅₀	71 1 Compound: 23bb	Cytotoxicity against human Ramos cells after 72 hrs by MTT assay Cytotoxicity against human Ramos cells after 72 hrs by MTT assay	[PMID: 26443078]
NCI-H460	IC₅₀	55 1 Compound: 23bb	Cytotoxicity against human H460 cells after 72 hrs by MTT assay Cytotoxicity against human H460 cells after 72 hrs by MTT assay	[PMID: 26443078]
RPMI-8226	IC₅₀	15 1 Compound: 23bb	Cytotoxicity against human RPMI8226 cells after 72 hrs by MTT assay Cytotoxicity against human RPMI8226 cells after 72 hrs by MTT assay	[PMID: 26443078]
MV4-11	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human MV4-11 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human MV4-11 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HeLa	EC₅₀	58.9 1 Compound: 23bb	Inhibition of HDAC6 in human HeLa cells assessed as induction of tubulin acetylation incubated for 6 hrs by cytoblot assay Inhibition of HDAC6 in human HeLa cells assessed as induction of tubulin acetylation incubated for 6 hrs by cytoblot assay	[PMID: 26443078]
RPMI-8226	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human RPMI-8226 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human RPMI-8226 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
MV4-11	IC₅₀	60 1 Compound: 23bb	Cytotoxicity against human MV4-11 cells after 72 hrs by MTT assay Cytotoxicity against human MV4-11 cells after 72 hrs by MTT assay	[PMID: 26443078]
MV4-11	IC₅₀	60 1 Compound: 23bb	Cytotoxicity against human MV4-11 cells after 72 hrs by MTT assay Cytotoxicity against human MV4-11 cells after 72 hrs by MTT assay	[PMID: 26443078]
K562	IC₅₀	63 1 Compound: 23bb	Cytotoxicity against human K562 cells after 72 hrs by MTT assay Cytotoxicity against human K562 cells after 72 hrs by MTT assay	[PMID: 26443078]
SK-BR-3	IC₅₀	22 1 Compound: 23bb	Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay	[PMID: 26443078]
NCI-H460	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human NCI-H460 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
SK-OV-3	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
NCI-H460	IC₅₀	55 1 Compound: 23bb	Cytotoxicity against human H460 cells after 72 hrs by MTT assay Cytotoxicity against human H460 cells after 72 hrs by MTT assay	[PMID: 26443078]
Ramos	IC₅₀	71 1 Compound: 23bb	Cytotoxicity against human Ramos cells after 72 hrs by MTT assay Cytotoxicity against human Ramos cells after 72 hrs by MTT assay	[PMID: 26443078]
SK-OV-3	IC₅₀	50 1 Compound: 23bb	Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay	[PMID: 26443078]
RPMI-8226	IC₅₀	15 1 Compound: 23bb	Cytotoxicity against human RPMI8226 cells after 72 hrs by MTT assay Cytotoxicity against human RPMI8226 cells after 72 hrs by MTT assay	[PMID: 26443078]
HT-29	IC₅₀	72 1 Compound: 23bb	Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay Cytotoxicity against human HT-29 cells after 72 hrs by MTT assay	[PMID: 26443078]
A549	IC₅₀	104 1 Compound: 23bb	Cytotoxicity against human A549 cells after 72 hrs by MTT assay Cytotoxicity against human A549 cells after 72 hrs by MTT assay	[PMID: 26443078]
U-266	IC₅₀	14 1 Compound: 23bb	Cytotoxicity against human U266 cells after 72 hrs by MTT assay Cytotoxicity against human U266 cells after 72 hrs by MTT assay	[PMID: 26443078]
RPMI-8226	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human RPMI-8226 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human RPMI-8226 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
U-266	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human U-266 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human U-266 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
HeLa	EC₅₀	1741 1 Compound: 23bb	Induction of histone H3 acetylation in human HeLa cells incubated for 6 hrs by cytoblot assay Induction of histone H3 acetylation in human HeLa cells incubated for 6 hrs by cytoblot assay	[PMID: 26443078]
Ramos	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human Ramos cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human Ramos cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
Ramos	IC₅₀	71 1 Compound: 23bb	Cytotoxicity against human Ramos cells after 72 hrs by MTT assay Cytotoxicity against human Ramos cells after 72 hrs by MTT assay	[PMID: 26443078]
SK-BR-3	IC₅₀	22 1 Compound: 23bb	Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay Cytotoxicity against human SKBR3 cells after 72 hrs by MTT assay	[PMID: 26443078]
SK-OV-3	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human SK-OV-3 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]
SK-OV-3	IC₅₀	50 1 Compound: 23bb	Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay Cytotoxicity against human SKOV3 cells after 72 hrs by MTT assay	[PMID: 26443078]
U-266	IC₅₀	14 1 Compound: 23bb	Cytotoxicity against human U266 cells after 72 hrs by MTT assay Cytotoxicity against human U266 cells after 72 hrs by MTT assay	[PMID: 26443078]
U-266	IC₅₀	31 1 Compound: 27; SKLB-23bb	Antiproliferative activity against human U-266 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay Antiproliferative activity against human U-266 cells assessed as cell growth inhibition measured after 72 hrs by MTT assay	[PMID: 34101463]

In Vitro

SKLB-23bb (Compound 23bb) presents low nanomolar antiproliferative effects against panel of cancer cell lines. The antiproliferative activity is ton human malignant melanoma A375 cells and cervical cancer HeLa cells, SKLB-23bb shows the most potent activities with IC₅₀ values of 50 and 49 nM on A375 and HeLa cells, respectively. The antiproliferative activities against 11 kinds of hematological tumors (myelomaU266, RPMI8226 cells, human leukemia MV4-11, K562 cells, and human B cell lymphoma Ramos cells) or solid tumors (ovarian cancer A2780s, SKOV-3 cells, breast cancer SKBR3 cells, liver cancer HepG2 cells, lung cancer H460, A549 cells, cervical cancer HeLa cells and colon cancer HCT116, HT29 cells) cell lines of SKLB-23bb are evaluated by MTT, and the SAHA and ACY-1215 are as positive control. SKLB-23bb shows significant antiproliferative potential with the IC₅₀ values ranging from 14 to 104 nM in these tumor cell lines^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

SKLB-23bb (Compound 23bb) reduces the tumor growth in both the hematological tumor MV4-11 xenograft model and solid tumor HCT116 xenograft model. The significant antitumor activities are observed by intravenous administration of SKLB-23bb at 50 mg/kg on MV4-11 and HCT116 xenograft models. The growth of MV4-11 and HCT116 cancer cell xenografts is suppressed by 55.0% and 76.3% (percent of tumor mass change [TGI] values) after iv administration of SKLB-23bb at 50 mg/kg. The HCT116 xenograft model is also established to investigate the antitumor activity of oral administration of SKLB-23bb. The TGI value of oral administration of SKLB-23bb (25 mg/kg) on HCT116 xenograft model is 60.4%, which is superior to the SAHA group (100 mg/kg, 59.2%). Additionally, the body weight decrease is acceptable and no other adverse effects are observed upon treatment with SKLB-23bb. Low clearance (CL=7.008 L/kg per hour for iv, CL=12.877 L/kg per hour for po) and long terminal half-life (t_1/2=7.658 h for iv, t_1/2=9.62 h for po) are observed in SKLB-23bb. The oral bioavailability of SKLB-23bb is excellent in rats and the bioavailability is up to 47.0%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

396.44

Formula

C₂₁H₂₄N₄O₄

CAS No.

1815580-06-3

Appearance

Solid

Color

Light yellow to yellow

SMILES

CN(C1=CC(OCCCC(NO)=O)=C(OC)C=C1)C2=NC(C)=NC3=CC=CC=C32

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 32 mg/mL (80.72 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5224 mL	12.6122 mL	25.2245 mL
5 mM	0.5045 mL	2.5224 mL	5.0449 mL
10 mM	0.2522 mL	1.2612 mL	2.5224 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (6.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (6.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.31 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 98.0%

Select Batch:

Data Sheet (278 KB) SDS (254 KB)

COA (247 KB) HNMR (252 KB)

Handling Instructions (2659 KB)

References

[1]. Yang Z, et al. Discovery of Selective Histone Deacetylase 6 Inhibitors Using the Quinazoline as the Cap for the Treatment of Cancer. J Med Chem. 2016 Feb 25;59(4):1455-70. [Content Brief]

Cell Assay ^[1]	A375, A2780s, SKBR3, HepG2, HeLa, HCT116, A549, and SKOV-3 cells are cultured in DMEM. RPMI8226, K562, H460, HT29, and Ramos cells are cultured in RPMI-1640 medium. MV4-11 cells are cultured in IMDM. All media contains 10% fetal bovine serum (FBS), 100 units/mL Penicillin, and 100 μg/mL Streptomycin. Cells are incubated at 37 °C in a humidified atmosphere of 5% CO₂. Cells in logarithmic phase are seeded into 96-well culture plates at densities of 3000-5000 cells per well and subsequently treated with various concentrations of compounds (e.g., SKLB-23bb;10, 100, 1000, and 10000 nM) for 72 h in final volumes of 200 μL. Upon end point, 20 μL of MTT (5 mg/mL) is added to each well, and the cells are incubated for an additional 1-3 h. After carefully removal of the medium, the precipitates are dissolved in 150 μL of DMSO via mechanically shaking, and then absorbance values at a wavelength of 570 nm are taken on a spectrophotometer. IC₅₀ values are calculated using percentage of growth versus untreated control^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Mice^[1] For the MV4-11 and Ramos xenograft models, MV4-11 and Ramos cells (107 cells in 100 μL of serum-free IMDM) are injected subcutaneously into the right flanks of 5- to 6-week-old female NOD/SCID mice. For the HCT116 xenograft, HCT116 cells (107 cells in 100 μL of serum-free DMEM) are injected subcutaneously into the right flanks of 5- to 6-week-old female Balb/c nude mice. When the size of the formed xenografts reach 100-150 mm3, the mice are randomly divided (6 mice per group in MV4-11 model, 8 mice per group in Ramos model, and 7 mice per group in HCT116 model) into control group and treated groups. The mice in the experimental groups receive intravenous (iv) injection (50 mg/kg) or oral administration (25 mg/kg) of SKLB-23bb every 2 days. The mice in the vehicle group receive iv injection or oral administration of equal amount of physiological saline containing 5% ethanol and 5% Cremophor EL. Those in the SAHA or LBH-589 or ACY-1215 groups (positive controls) receive ip injection (50 mg/kg for SAHA and 10 mg/kg for LBH-589, dissolved in physiological saline containing 10% DMSO and 45% PEG400 to a concentration of 10 mg/mL) or oral administration (100 mg/kg for SAHA and 40 mg/kg for ACY-1215, dissolved in the same way described above) every 2 days. Tumor burden is measured every 2 days by a caliper. Tumor volume (TV) is calculated. The day that treatment started is defined as day 0. At the end of the experiment, mice are sacrificed and tumors are collected and weighed^[1]. Rats^[1] SKLB-23bb is administered to SD rats ntravenously (iv) at 12 mg/kg body weight and orally at 12 mg/kg body weight. Blood samples are taken, and the plasma is analyzed for concentration of SKLB-23bb using an LC-MS/MS system^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Yang Z, et al. Discovery of Selective Histone Deacetylase 6 Inhibitors Using the Quinazoline as the Cap for the Treatment of Cancer. J Med Chem. 2016 Feb 25;59(4):1455-70. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5224 mL	12.6122 mL	25.2245 mL	63.0612 mL
	5 mM	0.5045 mL	2.5224 mL	5.0449 mL	12.6122 mL
	10 mM	0.2522 mL	1.2612 mL	2.5224 mL	6.3061 mL
	15 mM	0.1682 mL	0.8408 mL	1.6816 mL	4.2041 mL
	20 mM	0.1261 mL	0.6306 mL	1.2612 mL	3.1531 mL
	25 mM	0.1009 mL	0.5045 mL	1.0090 mL	2.5224 mL
	30 mM	0.0841 mL	0.4204 mL	0.8408 mL	2.1020 mL
	40 mM	0.0631 mL	0.3153 mL	0.6306 mL	1.5765 mL
	50 mM	0.0504 mL	0.2522 mL	0.5045 mL	1.2612 mL
	60 mM	0.0420 mL	0.2102 mL	0.4204 mL	1.0510 mL
	80 mM	0.0315 mL	0.1577 mL	0.3153 mL	0.7883 mL

SKLB-23bb Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

SKLB-23bb1815580-06-3HDACHistone deacetylasesInhibitorinhibitorinhibit

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