1. Cell Cycle/DNA Damage
    Epigenetics
  2. PARP

INO-1001 (Synonyms: 3-Aminobenzamide)

Cat. No.: HY-12022 Purity: 99.92%
Data Sheet SDS Handling Instructions

INO-1001 is a potent inhibitor of PARP with IC50 of < 50 nM in CHO cells, and acts as a mediator of oxidant-induced myocyte dysfunction during reperfusion. 

For research use only. We do not sell to patients.
INO-1001 Chemical Structure

INO-1001 Chemical Structure

CAS No. : 3544-24-9

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO $55 In-stock
200 mg $50 In-stock
500 mg $80 In-stock
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    INO-1001 purchased from MCE. Usage Cited in: Biochim Biophys Acta. 2016 Dec;1863(12):3027-3039.

    The protein expression of PAR and PARP1, FoxO3 and phosphorylated FoxO3 (T32, S252 and S314), and FoxO3 in the nucleus and cytoplasm is measured byWestern blotting. The results are normalized to those of α-tubulin/Lamin B1 and are presented as the means ± SEM.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    INO-1001 is a potent inhibitor of PARP with IC50 of < 50 nM in CHO cells, and acts as a mediator of oxidant-induced myocyte dysfunction during reperfusion. 

    IC50 & Target

    IC50: 50 nM (PARP)

    In Vitro

    INO-1001 (>1 μM) leads to more than 95% inhibition of PARP activity without significant cellular toxicity. In addition, INO-1001 significantly sensitizes CHO cells by blocking most of the DNA repair occurring between radiation fractions[1]. Inhibition of PARP activity by INO-1001 significantly improves endothelial function by enhancing the acetylcholine-induced, endothelium-dependent, nitric oxide mediated vasorelaxation after exposure with 400 μM H2O2[2].

    In Vivo

    In a db/db (Leprdb/db) mouse model, INO-1001 ameliorates diabetes-induced albumin excretion and mesangial expansion, and also decreases diabetes-induced podocyte depletion[3]. Treatment with INO-1001 (1.6 mg/kg via intracerebral injection) prevents NAD+ depletion and improves water maze performance after controlled cortical impact (CCI) in mice[4].

    References
    Preparing Stock Solutions
    Concentration Volume Mass 1 mg 5 mg 10 mg
    1 mM 7.3448 mL 36.7242 mL 73.4484 mL
    5 mM 1.4690 mL 7.3448 mL 14.6897 mL
    10 mM 0.7345 mL 3.6724 mL 7.3448 mL
    Please refer to the solubility information to select the appropriate solvent.
    Kinase Assay
    [1]

    PARP activity is measured with a PARP Activity Assay Kit. This method measures relative PARP activity by determining the level of incorporation of 3H-NAD into trichloroacetic acid (TCA) precipitable material in the presence of sheared genomic DNA, which activates PARP. The reaction mixture is added directly to washed cultures in 12-well culture plates and the reaction is allowed to proceed for 60 minutes at 37°C before the cells are removed mechanically, transferred to a microcentrifuge tube, and precipitated with ice-cold 5% TCA. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    INO-1001 is formulated in sterile water sweetened with NutraSweet.

    Male db/db (Leprdb/db) mice, together with nondiabetic control db/m mice on C57BLKs/J background, are used. INO-1001 and PJ-34 treatment are initiated at 5 weeks of age. In sterile water that is sweetened with NutraSweet, 4.8 g/L INO-1001 and 2.4 g/L PJ-34 is dissolved. Control animals receive sweetened water only. The average inhibitor consumption is 60 mg/kg INO-1001 and 30 mg/kg PJ-34. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    136.15

    Formula

    C₇H₈N₂O

    CAS No.

    3544-24-9

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Solvent & Solubility

    10 mM in DMSO

    * "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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    Product Name:
    INO-1001
    Cat. No.:
    HY-12022
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