1. Cell Cycle/DNA Damage
    Epigenetics
  2. PARP
  3. 3-Aminobenzamide

3-Aminobenzamide (Synonyms: PARP-IN-1)

Cat. No.: HY-12022 Purity: 99.92%
Handling Instructions

3-Aminobenzamide is a potent inhibitor of PARP with IC50 of appr 50 nM in CHO cells, and acts as a mediator of oxidant-induced myocyte dysfunction during reperfusion.

For research use only. We do not sell to patients.

3-Aminobenzamide Chemical Structure

3-Aminobenzamide Chemical Structure

CAS No. : 3544-24-9

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Customer Review

Based on 12 publication(s) in Google Scholar

Top Publications Citing Use of Products

    3-Aminobenzamide purchased from MCE. Usage Cited in: Biochim Biophys Acta. 2016 Dec;1863(12):3027-3039.

    The protein expression of PAR and PARP1, FoxO3 and phosphorylated FoxO3 (T32, S252 and S314), and FoxO3 in the nucleus and cytoplasm is measured byWestern blotting. The results are normalized to those of α-tubulin/Lamin B1 and are presented as the means ± SEM.

    3-Aminobenzamide purchased from MCE. Usage Cited in: Mol Cell Endocrinol. 2018 Oct 15;474:137-150.

    Rat heart tissues, which are injected with Ad-PARP1 or treated with 3AB following AAC surgery, the nuclear fraction are immunoprecipitated with anti-PARP1 antibody and subsequently subjected to immunoblotting analysis. n=3.

    3-Aminobenzamide purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.

    The protein expression of PARP1 and the phosphorylation levels of mTOR target substrates, including S6K1, 4E-BP, and ULK1, are partially reversed in the abdominal aortic constriction (AAC) model after treatment with 3AB or Rapamycin.

    3-Aminobenzamide purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.

    3-Aminobenzamide (3AB; 10 uM) clearly attenuates the cardiac fibrosis triggered by TGF-β1, as demonstrated by the reduction in protein levels of FN and Col I .

    3-Aminobenzamide purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.

    In the 3-Aminobenzamide (3AB) plus TGF-β1 group, the phosphorylation of target substrates of mTOR (S6K1 and 4E-BP) is conformably lower than TGF-β1 treatment, as shown by western blotting.

    3-Aminobenzamide purchased from MCE. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.

    In the 3-Aminobenzamide (3AB) plus TGF-β1 group, the phosphorylation of target substrates of mTOR (ULK1) is conformably lower than TGF-β1 treatment, as shown by western blotting.
    • Biological Activity

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    Description

    3-Aminobenzamide is a potent inhibitor of PARP with IC50 of appr 50 nM in CHO cells, and acts as a mediator of oxidant-induced myocyte dysfunction during reperfusion.

    IC50 & Target

    PARP

    50 nM (IC50)

    In Vitro

    3-Aminobenzamide (>1 μM) causes more than 95% inhibition of PARP activity without significant cellular toxicity. INO-1001 significantly sensitizes CHO cells by blocking most of the DNA repair occurring between radiation fractions[1]. 3-Aminobenzamide significantly improves endothelial function by enhancing the acetylcholine-induced, endothelium-dependent, nitric oxide mediated vasorelaxation after exposure with 400 μM H2O2[2].

    In Vivo

    In a db/db (Leprdb/db) mouse model, 3-Aminobenzamide ameliorates diabetes-induced albumin excretion and mesangial expansion, and also decreases diabetes-induced podocyte depletion[3]. 3-Aminobenzamide (1.6 mg/kg via intracerebral injection) prevents NAD+ depletion and improves water maze performance after controlled cortical impact (CCI) in mice[4].

    Molecular Weight

    136.15

    Formula

    C₇H₈N₂O

    CAS No.

    3544-24-9

    SMILES

    O=C(C1=CC=CC(N)=C1)N

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 11.11 mg/mL (81.60 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 7.3448 mL 36.7242 mL 73.4484 mL
    5 mM 1.4690 mL 7.3448 mL 14.6897 mL
    10 mM 0.7345 mL 3.6724 mL 7.3448 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Kinase Assay
    [1]

    PARP activity is measured with a PARP Activity Assay Kit. This method measures relative PARP activity by determining the level of incorporation of 3H-NAD into trichloroacetic acid (TCA) precipitable material in the presence of sheared genomic DNA, which activates PARP. The reaction mixture is added directly to washed cultures in 12-well culture plates and the reaction is allowed to proceed for 60 minutes at 37°C before the cells are removed mechanically, transferred to a microcentrifuge tube, and precipitated with ice-cold 5% TCA.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    Male db/db (Leprdb/db) mice, together with nondiabetic control db/m mice on C57BLKs/J background, are used. INO-1001 and PJ-34 treatment are initiated at 5 weeks of age. In sterile water that is sweetened with NutraSweet, 4.8 g/L 3-Aminobenzamide and 2.4 g/L PJ-34 is dissolved. Control animals receive sweetened water only. The average inhibitor consumption is 60 mg/kg 3-Aminobenzamide and 30 mg/kg PJ-34.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Product Name:
    3-Aminobenzamide
    Cat. No.:
    HY-12022
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