1. Cell Cycle/DNA Damage Epigenetics
  2. PARP
  3. 3-Aminobenzamide

3-Aminobenzamide  (Synonyms: PARP-IN-1)

Cat. No.: HY-12022 Purity: 99.99%
COA Handling Instructions

3-Aminobenzamide (PARP-IN-1) is a potent inhibitor of PARP with IC50 of appr 50 nM in CHO cells, and acts as a mediator of oxidant-induced myocyte dysfunction during reperfusion.

For research use only. We do not sell to patients.

3-Aminobenzamide Chemical Structure

3-Aminobenzamide Chemical Structure

CAS No. : 3544-24-9

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
USD 28 In-stock
Solution
10 mM * 1 mL in Water USD 28 In-stock
Solid
200 mg USD 25 In-stock
500 mg USD 40 In-stock
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5 g   Get quote  

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Customer Review

Based on 14 publication(s) in Google Scholar

Top Publications Citing Use of Products

    3-Aminobenzamide purchased from MedChemExpress. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.  [Abstract]

    In the 3-Aminobenzamide (3AB) plus TGF-β1 group, the phosphorylation of target substrates of mTOR (ULK1) is conformably lower than TGF-β1 treatment, as shown by western blotting.

    3-Aminobenzamide purchased from MedChemExpress. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.  [Abstract]

    3-Aminobenzamide (3AB; 10 uM) clearly attenuates the cardiac fibrosis triggered by TGF-β1, as demonstrated by the reduction in protein levels of FN and Col I .

    3-Aminobenzamide purchased from MedChemExpress. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.  [Abstract]

    The protein expression of PARP1 and the phosphorylation levels of mTOR target substrates, including S6K1, 4E-BP, and ULK1, are partially reversed in the abdominal aortic constriction (AAC) model after treatment with 3AB or Rapamycin.

    3-Aminobenzamide purchased from MedChemExpress. Usage Cited in: J Cell Biochem. 2019 Apr;120(4):4813-4826.  [Abstract]

    In the 3-Aminobenzamide (3AB) plus TGF-β1 group, the phosphorylation of target substrates of mTOR (S6K1 and 4E-BP) is conformably lower than TGF-β1 treatment, as shown by western blotting.

    3-Aminobenzamide purchased from MedChemExpress. Usage Cited in: Mol Cell Endocrinol. 2018 Oct 15;474:137-150.  [Abstract]

    Rat heart tissues, which are injected with Ad-PARP1 or treated with 3AB following AAC surgery, the nuclear fraction are immunoprecipitated with anti-PARP1 antibody and subsequently subjected to immunoblotting analysis. n=3.

    3-Aminobenzamide purchased from MedChemExpress. Usage Cited in: Biochim Biophys Acta. 2016 Dec;1863(12):3027-3039.  [Abstract]

    The protein expression of PAR and PARP1, FoxO3 and phosphorylated FoxO3 (T32, S252 and S314), and FoxO3 in the nucleus and cytoplasm is measured byWestern blotting. The results are normalized to those of α-tubulin/Lamin B1 and are presented as the means ± SEM.

    View All PARP Isoform Specific Products:

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    3-Aminobenzamide (PARP-IN-1) is a potent inhibitor of PARP with IC50 of appr 50 nM in CHO cells, and acts as a mediator of oxidant-induced myocyte dysfunction during reperfusion.

    IC50 & Target[1]

    PARP

    50 nM (IC50)

    In Vitro

    3-Aminobenzamide (PARP-IN-1) (>1 μM) causes more than 95% inhibition of PARP activity without significant cellular toxicity. INO-1001 significantly sensitizes CHO cells by blocking most of the DNA repair occurring between radiation fractions[1]. 3-Aminobenzamide significantly improves endothelial function by enhancing the acetylcholine-induced, endothelium-dependent, nitric oxide mediated vasorelaxation after exposure with 400 μM H2O2[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    In a db/db (Leprdb/db) mouse model, 3-Aminobenzamide ameliorates diabetes-induced albumin excretion and mesangial expansion, and also decreases diabetes-induced podocyte depletion[3]. 3-Aminobenzamide (1.6 mg/kg via intracerebral injection) prevents NAD+ depletion and improves water maze performance after controlled cortical impact (CCI) in mice[4].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    136.15

    Formula

    C7H8N2O

    CAS No.
    Appearance

    Solid

    Color

    Off-white to light brown

    SMILES

    O=C(C1=CC=CC(N)=C1)N

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 2 years
    -20°C 1 year
    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 11.11 mg/mL (81.60 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 7.3448 mL 36.7242 mL 73.4484 mL
    5 mM 1.4690 mL 7.3448 mL 14.6897 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 25 mg/mL (183.62 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.99%

    References
    Kinase Assay
    [1]

    PARP activity is measured with a PARP Activity Assay Kit. This method measures relative PARP activity by determining the level of incorporation of 3H-NAD into trichloroacetic acid (TCA) precipitable material in the presence of sheared genomic DNA, which activates PARP. The reaction mixture is added directly to washed cultures in 12-well culture plates and the reaction is allowed to proceed for 60 minutes at 37°C before the cells are removed mechanically, transferred to a microcentrifuge tube, and precipitated with ice-cold 5% TCA.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    Male db/db (Leprdb/db) mice, together with nondiabetic control db/m mice on C57BLKs/J background, are used. INO-1001 and PJ-34 treatment are initiated at 5 weeks of age. In sterile water that is sweetened with NutraSweet, 4.8 g/L 3-Aminobenzamide and 2.4 g/L PJ-34 is dissolved. Control animals receive sweetened water only. The average inhibitor consumption is 60 mg/kg 3-Aminobenzamide and 30 mg/kg PJ-34.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 7.3448 mL 36.7242 mL 73.4484 mL 183.6210 mL
    5 mM 1.4690 mL 7.3448 mL 14.6897 mL 36.7242 mL
    10 mM 0.7345 mL 3.6724 mL 7.3448 mL 18.3621 mL
    15 mM 0.4897 mL 2.4483 mL 4.8966 mL 12.2414 mL
    20 mM 0.3672 mL 1.8362 mL 3.6724 mL 9.1811 mL
    25 mM 0.2938 mL 1.4690 mL 2.9379 mL 7.3448 mL
    30 mM 0.2448 mL 1.2241 mL 2.4483 mL 6.1207 mL
    40 mM 0.1836 mL 0.9181 mL 1.8362 mL 4.5905 mL
    50 mM 0.1469 mL 0.7345 mL 1.4690 mL 3.6724 mL
    60 mM 0.1224 mL 0.6121 mL 1.2241 mL 3.0604 mL
    80 mM 0.0918 mL 0.4591 mL 0.9181 mL 2.2953 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    3-Aminobenzamide Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    3-Aminobenzamide
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