Anti-Mouse CSF1 Antibody (5A1)
Based on 1 Customer Validation
Anti-Mouse CSF1 Antibody (5A1) is a rat-derived anti-mouse CSF1 IgG1 κ type antibody inhibitor. Anti-Mouse CSF1 Antibody (5A1) can inhibit cells proliferation and macrophage-induced invasion. Anti-Mouse CSF1 Antibody (5A1) shows potent anti-tumor effect in various tumor models, such as neuroblastoma.
For research use only. We do not sell to patients.
- Purity: 96.799%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Rat IgG1 kappa
Mouse
CSF1
Anti-Mouse CSF1 Antibody (5A1) (0-50 μg, 0-48 h) inhibits proliferation and activation of AKT and S6 in monocyte-derived cells [4].
Anti-Mouse CSF1 Antibody (5A1) (2.5 μg/ml, 96 h) significantly reduces macrophage-induced invasion in human MCF-7 cells and murine 410.4 cells [4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Monocyte-derived cells
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Concentration:0, 2.5, 10 and 50 μg
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Incubation Time:0, 12, 24, 36 and 48 h
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Result:Inhibited proliferation at the lowest concentration.
Anti-Mouse CSF1 Antibody (5A1) (0.5 mg/week, i.p., 4weeks) prevents bone loss in ovariectomy-induced mice models[2].
Anti-Mouse CSF1 Antibody (5A1) (10 mg/kg, i.p., twice a week) increases suppression of tumor growth and survival companied with anti-CSF-1 mAbs MCS110 (HY-P99368) and CSF-1R inhibitor BLZ945 (HY-12768) in neuroblastoma mice models[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Mice were injected with MGS1 cells into the right flank[1]
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Dosage:200 μg
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Administration:Intraperitoneally injection, every 3-4 days
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Result:Significantly reduced MGS1 tumor growth.
Significantly increased the survival of mice injected intracranially with MGS1.
Downregulated of myeloid-associated genes, including CSF1R, CCR1, C1qa, and CX3CR1.
Upregulated gene sets involved in negative regulation of cell migration/proliferation and extracellular matrix organization.
Decreased CD11b+ F4-80+ cells.
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Animal Model:Ovariectomy-induced mice models[2]
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Dosage:0.5 mg/week
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Administration:Intraperitoneally injection, 4weeks
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Result:Prevented bone loss and increased DPD excretion.
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Animal Model:CHLA-255-Fluc neuroblastoma cells and human monocytes tumor mice model[3]
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Dosage:10 mg/kg
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Administration:Intraperitoneally injection, twice a week, companied with anti-CSF-1 mAbs MCS110 (HY-P99368) and CSF-1R inhibitor BLZ945 (HY-12768)
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Result:Reduced the frequency of these F4/80+ mouse cells and CD14+ human cells.
Reduced CD163+ human monocyte-derived cells.
Increased suppression of tumor growth and survival of mice.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Product Image
in vivo CSF1 neutralization
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse CSF1 Antibody (5A1)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Yeung J, et al. Targeting the CSF1/CSF1R axis is a potential treatment strategy for malignant meningiomas. Neuro Oncol. 2021 Nov 2;23(11):1922-1935. [Content Brief]
[2]. Cenci S, Weitzmann MN, Gentile MA, Aisa MC, Pacifici R. M-CSF neutralization and egr-1 deficiency prevent ovariectomy-induced bone loss. J Clin Invest. 2000 May;105(9):1279-87. [Content Brief]
[3]. Webb MW, et al. Colony stimulating factor 1 receptor blockade improves the efficacy of chemotherapy against human neuroblastoma in the absence of T lymphocytes. Int J Cancer. 2018 Sep 15;143(6):1483-1493. [Content Brief]
[4]. Rietkötter E, et al. Anti-CSF-1 treatment is effective to prevent carcinoma invasion induced by monocyte-derived cells but scarcely by microglia. Oncotarget. 2015 Jun 20;6(17):15482-93. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse CSF1 Antibody (5A1) Related Classifications
HY-P990257 Related Classifications
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