Anti-Mouse CSF1R/CD115 Antibody (AFS98)
Based on 1 Customer Validation
Anti-Mouse (CSF1R/CD115) Antibody (AFS98) is an anti-mouse CSF1R IgG2a antibody inhibitor derived from rats. Anti-Mouse (CSF1R/CD115) Antibody (AFS98) inhibits M-CSF-dependent colony formation and cell growth by blocking the binding of M-CSF to its receptor. Anti-Mouse (CSF1R/CD115) Antibody (AFS98) can be used for the researches of inflammation, metabolic disease, cardiovascular disease and neurological disease, such as such as stroke, diabetes and arthritis.
For research use only. We do not sell to patients.
- Purity: 99%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Rat IgG2a kappa
Mouse
CSF1R/CD115
The antibody framework is stable, specific and adaptable, and has the ability to bind both antigens and endogenous immune receptors. Monoclonal antibodies have several derivatives, including bispecific antibodies, antibody-drug conjugates, and antibody fragments, and have significant effects in fields such as immunology and oncology. When designing inhibitory antibodies, considerations include identification of antigen-specific variable regions, choice of expression system, use of multispecific formats, and antibody derivatives based on fragmentation, oligomerization, or conjugation with other functional moieties[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Anti-Mouse (CSF1R/CD115) Antibody (AFS98) (25 mg/kg, i.p., every second day for 6 weeks) suppresses the progression of established diabetic nephropathy[2].
in Obese (db/db) mice by targeting macrophage-mediated injury[2].
Anti-Mouse (CSF1R/CD115) Antibody (AFS98) (2 mg/mouse, i.p., every second day for 6 weeks) acts as protection from early atherogenesis in apoE-deficient mice[3].
Anti-Mouse (CSF1R/CD115) Antibody (AFS98) (25 or 50 mg/kg, i.p., every day for 14 days) shows significant antiinflammatory effects in Collagen-induced arthritis (CIA) mice models[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Obese (db/db) mice models (C57BL/KS, 12 weeks)[2]
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Dosage:25 mg/kg
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Administration:Intraperitoneal injections, every second day for 6 weeks
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Result:Reduced renal hypertrophy, but not obesity, diabetes or circulating monocytes.
Reduced macrophage accumulation in diabetic kidneys.
Suppressed diabetic glomerular hyperfiltration without altering albuminuria.
Reduced ubular damage and tubulo-interstitial fibrosis.
Upregulated the levels of phospho-ERK, phospho-p38, phospho-JNK, phospho-ATF2 and α-tubulin.
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Animal Model:ApoE-deficient mice fed a high-fat diet from 6 to 12 weeks of age[3]
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Dosage:2 mg/mouse
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Administration:Intraperitoneal injections, every second day for 6 weeks
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Result:Prevented development of fatty streaks.
Suppressed the accumulation of macrophage-derived foam cells in the aortic root by 70%.
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Animal Model:Collagen-induced arthritis (CIA) mice models (DBA/1, male, 7-9 weeks)[4]
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Dosage:25 or 50 mg/kg
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Administration:Intraperitoneal injections, every day for 14 days
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Result:Inhibitd inflammatory bone and cartilage loss.
Reduced synovial joint macrophage infiltrates and splenic monocyte recruitment.
Blocked systemic bone loss by blockade of osteoclast differentiation.
Protected against bone and cartilage erosion independent of effects on inflammation in serum-transfer arthritis.
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
in vivo macrophage depletion; in vitro CSF1R neutralization; in vivo monocyte depletion; Flow cyt
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Immobilized CSF1R Protein, Mouse (HEK293, His, HY-P72952) can bind Anti-Mouse CSF1R/CD115 Antibody (AFS98). The EC50 for this effect is 262.9 ng/mL.
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse CSF1R/CD115 Antibody (AFS98)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Marino Lee S, et al. Microglia depletion increase brain injury after acute ischemic stroke in aged mice. Exp Neurol. 2021 Feb;336:113530. [Content Brief]
[2]. Lim AK, et al. Antibody blockade of c-fms suppresses the progression of inflammation and injury in early diabetic nephropathy in obese db/db mice. Diabetologia. 2009 Aug;52(8):1669-79. [Content Brief]
[3]. Murayama T, et al. Intraperitoneal administration of anti-c-fms monoclonal antibody prevents initial events of atherogenesis but does not reduce the size of advanced lesions in apolipoprotein E-deficient mice. Circulation. 1999 Apr 6;99(13):1740-6. [Content Brief]
[4]. Toh ML, et al. Bone- and cartilage-protective effects of a monoclonal antibody against colony-stimulating factor 1 receptor in experimental arthritis. Arthritis Rheumatol. 2014 Nov;66(11):2989-3000. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse CSF1R/CD115 Antibody (AFS98) Related Classifications
HY-P990125 Related Classifications
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