Anti-Mouse TIM-1/CD365 Antibody (3B3) 

Anti-Mouse TIM-1/CD365 Antibody (3B3) is a rat-derived IgG2a κ type agonistic antibody, targeting to mouse TIM-1/CD365. Anti-Mouse TIM-1/CD365 Antibody (3B3) enhances T-cell proliferation and responses by forming a stable TIM-1 complex and bringing TIM-1 into the T-cell receptor (TCR)-CD3 complex. Anti-Mouse TIM-1/CD365 Antibody (3B3) can be used for the researches of cancer, inflammation and immunology, such as experimental autoimmune encephalomyelitis, B16 F10 tumor and transplant^[1][2][3][4].

Rat IgG2a kappa

Rat IgG2a kappa, Isotype Control

Mouse

TIM-1/CD365

Anti-Mouse TIM-1/CD365 Antibody (3B3) (10 μg/mL, 48 h) enhances T cell proliferation in response to ovalbumin and antigen-presenting cells ^[1].
Anti-Mouse TIM-1/CD365 Antibody (3B3) (10 μg/mL, 3 days) increases IL-4 levels in T_H2 cells^[1].
Anti-Mouse TIM-1/CD365 Antibody (3B3) (2.5 μg/mL, 2-4 days) results in a substantial increase in CD4⁺ and CD8⁺ T cell accumulation in mixed lymphocytes^[4].
Anti-Mouse TIM-1/CD365 Antibody (3B3) (2.5 μg/mL, 6 days) enhances proliferation of CD4⁺CD25⁺ C57BL/6 regulatory T cells stimulated with mature DBA/2 DC cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse TIM-1/CD365 Antibody (3B3) (100 μg, i.p., 1 d or 8 h before the initial exposure to OVA) abrogates respiratory tolerance induction and develops airway inflammation in ovalbumin (OVA)-induced airway hyperreactivity (AHR) mice models^[1].
Anti-Mouse TIM-1/CD365 Antibody (3B3) suppresses tumor growth and increases activated CD4⁺ and CD8⁺ T cells in B16 F10 tumor mice models^[2].
Anti-Mouse TIM-1/CD365 Antibody (3B3) (100 μg, i.p., every other day from day 0 to 8) enhances the severity in experimental autoimmune encephalomyelitis (EAE) mice models^[3].
Anti-Mouse TIM-1/CD365 Antibody (3B3) (250 μg, i.p., at days 0 and 2 after transplantation) negates prevent allograft rejection in MHC-mismatched islet transplant mice models^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

171283  [NCBI]

Q5QNS5-1

in vivo TIM-1 activation; in vitro T cell stimulation/activation; Functional assays; ELISA; Flow

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

150 kDa

[Anti-Mouse TIM-1/CD365 Antibody (3B3)]

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Umetsu SE, et al. TIM-1 induces T cell activation and inhibits the development of peripheral tolerance. Nat Immunol. 2005 May;6(5):447-54.  [Content Brief]

  [2]. Tian X, et al. B-cell immune checkpoint TIM-1: a potential target for tumour immunotherapy. Signal Transduct Target Ther. 2023 Oct 20;8(1):389.  [Content Brief]

  [3]. Xiao S, et al. Differential engagement of Tim-1 during activation can positively or negatively costimulate T cell expansion and effector function. J Exp Med. 2007 Jul 9;204(7):1691-702.  [Content Brief]

  [4]. Degauque N, et al. Immunostimulatory Tim-1-specific antibody deprograms Tregs and prevents transplant tolerance in mice. J Clin Invest. 2008 Feb;118(2):735-41.  [Content Brief]