Anti-Mouse TIM-3 Antibody (RMT3-23)
Based on 1 Customer Validation
Anti-Mouse TIM-3 Antibody (RMT3-23) is a kind of rat-derived anti-mouse TIM-3 IgG2a antibody inhibitor. Anti-Mouse TIM-3 Antibody (RMT3-23) blocks Tim-3 binding to phosphatidylserine (PtdSer) and carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1). Anti-Mouse TIM-3 Antibody (RMT3-23) inhibits phagocytosis of apoptotic cells in peritoneal exudate Mac1+ cells. Anti-Mouse TIM-3 Antibody (RMT3-23) can be used for the researches of inflammation and immunology, such as rheumatoid arthritis.
For research use only. We do not sell to patients.
- Purity: 99.64%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Rat IgG2a kappa
Mouse
TIM-3
Anti-Mouse TIM-3 Antibody (RMT3-23) (30 μg/mL, 1.5 h) inhibits approximately 50% recognition of apoptotic cells by CD8+ dendritic cells and reduces IFN-γproduction in splenic dendritic cells [1].
Anti-Mouse TIM-3 Antibody (RMT3-23) (30 mins) significantly inhibits phagocytosis of apoptotic cells in peritoneal exudate Mac1+ cells[1].
Anti-Mouse TIM-3 Antibody (RMT3-23) (10 μg/mL, 30 mins) blocks Tim-3 binding to phosphatidylserine (PtdSer) in thymocytes and carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1) in Jurkat T cells[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Anti-Mouse TIM-3 Antibody (RMT3-23) (0.25 mg/dose, i.p., 3 times/week from week 4) ameliorates disease by inhibiting the TIM-1 and -3 signaling pathway in a murine model of rheumatoid arthritis[2].
Anti-Mouse TIM-3 Antibody (RMT3-23) (200 μg, i.p.) down-modulates Tim-3 surface expression but does not deplete Tim-3C cells in Tim-3 transgenic mice models[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:B6 mice were transferred with CFSE-labeled OT-I cells (2 x 106 per mouse)[1]
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Dosage:200 μg/mouse
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Administration:Intraperitoneally injection, twice a week for 4 weeks.
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Result:Abrogated cells apoptosis induced by Tim-3.
Significantly increased TUNEL+ cells and serum level of anti-dsDNA antibodies.
Reduced proliferation of OT-I cells.
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Animal Model:Rheumatoid arthritis mice models (SKG mice, 8-9 weeks) [2]
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Dosage:0.25 mg/dose
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Administration:Intraperitoneally injection, 3 times/week from week 4
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Result:Exhibited signifcantly lower arthritis scores and histological scores in the radialcarpal joint and around proximal carpal bones with synovitis and erosions.
Significantly suppressed serum TNF-α level.
Reduced the mRNA expressions of cytokines IFN-γ, IL-6, and TNF-α.
Signifcantly decreased CD4+ T cells, CD11b+ cells, and CD11c+ cells.
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Animal Model:Tim-3 transgenic mice models (C57Bl/6)
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Dosage:200 μg
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Administration:v
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Result:Did not deplete Tim-3+ cells.
Significantly downregulated of Tim-3.
Q8VIM0-1
Unconjugated
The product can be reconstituted/diluted with sterile PBS or saline.
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Product Image
in vivo TIM-3 neutralization; in vitro TIM-3 blocking; Flow cytometry
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse TIM-3 Antibody (RMT3-23)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Nakayama M, et al. Tim-3 mediates phagocytosis of apoptotic cells and cross-presentation. Blood. 2009 Apr 16;113(16):3821-30. [Content Brief]
[2]. Nozaki Y, et al. Inhibition of the TIM-1 and -3 signaling pathway ameliorates disease in a murine model of rheumatoid arthritis. Clin Exp Immunol. 2024 Sep 16;218(1):55-64. [Content Brief]
[3]. Sabatos-Peyton CA, et al. Blockade of Tim-3 binding to phosphatidylserine and CEACAM1 is a shared feature of anti-Tim-3 antibodies that have functional efficacy. Oncoimmunology. 2017 Nov 9;7(2):e1385690. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse TIM-3 Antibody (RMT3-23) Related Classifications
HY-P990821 Related Classifications
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