Anti-Mouse CTLA-4 Antibody (9D9) 

Anti-Mouse CTLA-4 Antibody (9D9) is an anti-mouse CTLA-4 IgG2b monoclonal antibody. Anti-Mouse CTLA-4 Antibody (9D9) can bind to CTLA-4 and block its binding to B7. Anti-Mouse CTLA-4 Antibody (9D9) enhances T cell function by increasing the ratio of CD8⁺ T cells to regulatory T cells (Tregs). Anti-Mouse CTLA-4 Antibody (9D9) can be used for research on cancer such as colon cancer and melanoma^[1][2].

Mouse IgG2b kappa

Mouse IgG2b kappa, Isotype Control

Mouse

CTLA-4

Anti-Mouse CTLA-4 Antibody (9D9) (0.1-1000 nM, 2 h) has high binding strength with CTLA-4^WT (K_D = 15.4 nM) and CTLA-4^del36 (K_D = 69.6 nM) in HEK293-F cells^[1].
Anti-Mouse CTLA-4 Antibody (9D9) (0.01-1000 nM, 1 h) significantly blocks the binding of B7 to CTLA-4 in HEK293-F cells (IC₅₀ = 23.5 nM for ELISA, IC₅₀ = 24.4 nM for cell surface)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Anti-Mouse CTLA-4 Antibody (9D9) (200 μg, i.p. or i.t., on day 7, 10 and 14) has significant anti-tumor activity and increases CD4⁺/CD8⁺ T cell activation in female C57BL/6 mice bearing MC38 tumors^[1].
Anti-Mouse CTLA-4 Antibody (9D9) (100 μg/dose, i.p., on day 3, 6 and 9) has significant anti-tumor activity and prolongs survival in male C57BL/6 mice bearing B16-F10 tumors^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

12477  [NCBI]

P09793

in vivo CTLA-4 neutralization; Western blot; in vivo intra-tumoral regulatory T cell depletion

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

150 kDa

Liquid

Colorless to light yellow

[Anti-Mouse CTLA-4 Antibody (9D9)]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow Cytometry analysis of Raji cells labelling CTLA4 (red) with Anti-Mouse CTLA-4 Antibody (HY-P99132). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Mouse IgG H&L (AF488) (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG2b kappa (HY-P99982, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
• 
  Immobilized CTLA-4 Protein, Mouse (HEK293, His, HY-P71991) can bind Anti-Mouse CTLA-4 Antibody (9D9). The EC₅₀ for this effect is 1.979 ng/mL.

• [1]. Lax BM, et al. Both intratumoral regulatory T cell depletion and CTLA-4 antagonism are required for maximum efficacy of anti-CTLA-4 antibodies. Proc Natl Acad Sci U S A. 2023 Aug;120(31):e2300895120.  [Content Brief]

  [2]. Reilley MJ, et al. TLR9 activation cooperates with T cell checkpoint blockade to regress poorly immunogenic melanoma. J Immunother Cancer. 2019 Nov 26;7(1):323.  [Content Brief]