Anti-Mouse VISTA Antibody (13F3)
Based on 1 Customer Validation
Anti-Mouse VISTA Antibody (13F3) is an anti-mouse VISTA IgG monoclonal antibody. Anti-Mouse VISTA Antibody (13F3) can reverse the immunosuppressive effect of VISTA by blocking its binding to ligands such as VSIG3. Anti-Mouse VISTA Antibody (13F3) exacerbates pulmonary fibrosis (PF) by promoting Th17 cell differentiation. Anti-Mouse VISTA Antibody (13F3) can be used for research on cancer such as breast cancer and PF.
For research use only. We do not sell to patients.
- Purity : ≥95.0%
- Molecular Weight:150 kDa
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Isotype
Armenian hamster IgG
Recommend Isotype Controls
Species Reactivity
Mouse
IC50 & Target
VISTA
In Vivo
Anti-Mouse VISTA Antibody (13F3) (i.p., once every 3 days, from day 7 to day 21) aggravates pulmonary fibrosis in C57BL/6 mice with a Bleomycin hydrochloride (BLM) (HY-17565A) induced pulmonary fibrosis (PF) model[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:2×104 4T1 cells injected Balb/c mice[1]
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Dosage:50 μg
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Administration:intratumoral injection, on day 7, 9, 12, 14 and 16
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Result:Significantly inhibited tumor volume (inhibition rate of 53%).
Gene ID
Accession
Conjugated
Unconjugated
Reconsititution
The product can be reconstituted/diluted with sterile PBS or saline.
Application
in vivo blocking of VISTA signaling; in vitro blocking of VISTA signaling; Flow cytometry
Verified Bioactivity
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Immobilized VISTA/B7-H5 Protein, Mouse (159a.a, HEK293, His, HY-P72428) can bind Anti-Mouse VISTA Antibody (13F3). The EC50 for this effect is 71.89 ng/mL.
Chemical Information
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Appearance Liquid
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Molecular Weight 150 kDa
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Color Colorless to light yellow
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SMILES
[Anti-Mouse VISTA Antibody (13F3)]
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Formulation
Please refer to the lot-specific COA for specific buffer information.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (259 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Inhibitory Antibodies User Guide (603 KB)
References
[1]. Thakkar D, et al. Rationally targeted anti-VISTA antibody that blockades the C-C' loop region can reverse VISTA immune suppression and remodel the immune microenvironment to potently inhibit tumor growth in an Fc independent manner. J Immunother Cancer. 2022 Feb;10(2):e003382. [Content Brief]
[2]. Xie H, et al. VISTA Deficiency Exacerbates the Development of Pulmonary Fibrosis by Promoting Th17 Differentiation. J Inflamm Res. 2024 Jun 19;17:3983-3999. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Anti-Mouse VISTA Antibody (13F3) Related Classifications
HY-P990145 Related Classifications
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