Anti-Nicastrin Antibody (A5226A)

Anti-Nicastrin Antibody (A5226A) binds to human NCT at epitope R391, and interacts with both mature (active γ-secretase complex-associated) and immature forms of human NCT in NKO cells overexpressing human NCT^[1].
Anti-Nicastrin Antibody (A5226A) specifically recognizes active γ-secretase complexes containing mature NCT localized in CTB-positive lipid raft microdomains on the plasma membrane of HeLa cells and NKO cells expressing human NCT^[1].
Anti-Nicastrin Antibody (A5226A) dose-dependently inhibits γ-secretase activity in HEK293 cells stably expressing amyloid precursor protein- or Notch-derived γ-secretase substrates^[1].
Anti-Nicastrin Antibody (A5226A) inhibits γ-secretase activity in a cell-free in vitro assay by interfering with the interaction between NCT and Notch-based substrate N100^[1].
Anti-Nicastrin Antibody (A5226A) inhibits γ-secretase-dependent proliferation of A549 non-small cell lung cancer cells and DND-41 T-cell acute lymphoblastic leukemia cells, but does not affect HeLa cell viability^[1].
Anti-Nicastrin Antibody (A5226A) inhibits γ-secretase activity via substrate competition in cell-free assays and causes a moderate decrease in ICN1 in DND41 T-ALL cells in vitro^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.