Antibacterial agent 318 

Antibacterial agent 318 is an antibacterial agent that binds to bacterial DNA, blocks its replication, and forms supramolecular complexes. Antibacterial agent 318 acts as an oxidative stress inducer, elevating intracellular reactive oxygen species (ROS) levels, oxidizing glutathione (GSH) to glutathione disulfide (GSSG), depleting cellular GSH reserves, and inducing bacterial cell death through oxidative damage. Antibacterial agent 318 disrupts the bacterial cell membrane and reduces bacterial metabolic activity. Antibacterial agent 318 exhibits rapid bactericidal activity, inhibits bacterial biofilm formation, and displays minimal cytotoxicity toward non-cancerous mammalian cells. Antibacterial agent 318 is applicable in research on drug-resistant bacterial infections^[1].

Antibacterial agent 318 (compound 12f) inhibits the growth of E. faecalis, L. species, B. subtilis, S. aureus, E. coli, and S. marcescens with MIC values ranging from 1.56 to 6.25 μg/mL^[1].
Antibacterial agent 318 (1.56-12.48 μg/mL; 2.5-5 h) exhibits rapid bactericidal activity against E. faecalis, killing bacteria within 5 h at 1× MIC and 3.5 h at 8× MIC, with synergistic faster killing (2.5 h) when combined with Tetracycline (HY-A0107)^[1].
Antibacterial agent 318 (1.56-12.48 μg/mL) inhibits E. faecalis biofilm formation and auto-aggregation in a concentration-dependent manner, reducing formation by 83% at 8× MIC^[1].
Antibacterial agent 318 (1.56-12.48 μg/mL) reduces E. faecalis EPS content by 69.7% at 1× MIC and 74.4% at 8× MIC^[1].
Antibacterial agent 318 (30 μM) exhibits minimal cytotoxicity toward HEK293 cells, with viability above 87% at 30 μM^[1].
Antibacterial agent 318 (1.56 μg/mL) causes severe morphological damage to E. faecalis cells at 1× MIC, including ruptured cell walls and cytoplasmic leakage^[1].
Antibacterial agent 318 (1.56-12.48 μg/mL) can induce metabolic arrest in E. faecalis, induce E. faecalis to produce intracellular reactive oxygen species (ROS) in a concentration-dependent manner, and reduce GSH activity; it can also induce lipid peroxidation, increasing MDA levels up to 6-fold at a concentration of 8× MIC^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

611.67

C₃₅H₂₅N₅O₄S

O=C(N(C(C1C2(N3)C4=NC5=CC=CC=C5N=C4C6=C2C=CC=C6)=O)N7C(C8=CC=CC9=CC=CC(C7=O)=C98)=O)C1C3CCSC

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Jain A, et al. Unravelling the antibacterial potential and mechanistic insights of spirocyclic naphthalimide analogues against drug-resistant bacteria. Eur J Med Chem. 2026;304:118530.  [Content Brief]