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ATF3 Antibody (YA3844)

Customer Review

Based on 1 Customer Validation

ATF3 Antibody (YA3844) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATF3.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
ALL FC ICC
  • Experimental Validation Results for ATF3 Antibody (YA3844)
  • Experimental Validation Results for ATF3 Antibody (YA3844)
  • Experimental Validation Results for ATF3 Antibody (YA3844)
  • ATF3 Antibody (YA3844) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

ATF3 Antibody (YA3844) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ATF3.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 21 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 21 kDa
Immunogen

Purified recombinant fragment of human ATF3 (AA: 1-181) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718847

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ATF3 Antibody (YA3844)
    Flow cytometric analysis of 1X106 HeLa cells labeling ATF3 Antibody (HY-P84147, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for ATF3 Antibody (YA3844)
    Immunocytochemistry analysis of A549 cells labeling ATF3 with ATF3 Antibody (HY-P84147) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATF3 Antibody (HY-P84147) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATF3 Antibody (YA3844)
    Immunocytochemistry analysis of HeLa cells labeling ATF3 with ATF3 Antibody (HY-P84147) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATF3 Antibody (HY-P84147) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    ATF3 is a This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter; Activates transcription presumably by sequestering inhibitory cofactors away from the promoters; Stress-induced isoform, counteracts the transcriptional repression of isoform 1

  • Subcellular Localization

    Nucleus

  • Isoforms & Post-Translational Modification

    P18847 has 5 isomers: P18847-1: 20576 Da (predicted); P18847-2: 13266 Da (predicted); P18847-3: 15250 Da (predicted); P18847-4: 12261 Da (predicted); P18847-5: 14314 Da (predicted).

  • Subunit

    Binds DNA as a homodimer or a heterodimer. Interacts with KAT5; promoting KAT5 autoacetylation and KAT5 deubiquitination by USP7 (PubMed:25865756)

  • SwissProt ID

    P18847

  • Gene ID
    467 [NCBI]

ATF3 Antibody (YA3844) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling ATF3 Antibody (HY-P84147, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling ATF3 with ATF3 Antibody (HY-P84147) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATF3 Antibody (HY-P84147) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling ATF3 with ATF3 Antibody (HY-P84147) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATF3 Antibody (HY-P84147) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATF3 Antibody (YA3844) Product Image
    1/16
  • Experimental Validation Results for ATF3 Antibody (YA3844)
  • Experimental Validation Results for ATF3 Antibody (YA3844)
  • Experimental Validation Results for ATF3 Antibody (YA3844)
  • ATF3 Antibody (YA3844) Product Image
  • Experimental Validation Results for ATF3 Antibody (YA3844)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X106 HeLa cells labeling ATF3 Antibody (HY-P84147, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
  • Experimental Validation Results for ATF3 Antibody (YA3844)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling ATF3 with ATF3 Antibody (HY-P84147) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATF3 Antibody (HY-P84147) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATF3 Antibody (YA3844)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HeLa cells labeling ATF3 with ATF3 Antibody (HY-P84147) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATF3 Antibody (HY-P84147) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for ATF3 Antibody (YA3844)
  • Experimental Validation Results for ATF3 Antibody (YA3844)
  • Experimental Validation Results for ATF3 Antibody (YA3844)
  • ATF3 Antibody (YA3844) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X106 HeLa cells labeling ATF3 Antibody (HY-P84147, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/50 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti- Mouse IgG H&L (HY-P8005) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Mouse IgG Isotype Control (HY-P80757, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • ATF3 Antibody (YA3844) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling ATF3 with ATF3 Antibody (HY-P84147) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATF3 Antibody (HY-P84147) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATF3 Antibody (YA3844) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HeLa cells labeling ATF3 with ATF3 Antibody (HY-P84147) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with ATF3 Antibody (HY-P84147) at 1/500 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • ATF3 Antibody (YA3844) Product Image FC
  • ATF3 Antibody (YA3844) Product Image ICC
  • ATF3 Antibody (YA3844) Product Image ICC