CD300F Antibody (YA4381)
(Synonyms: CLM1; NKIR; CLM-1; IREM1; LMIR3; CD300lf; IREM-1; IgSF13)CD300F Antibody (YA4381) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD300F.
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Host:
Mouse
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Isotype:
IgG2b
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Application:
IHC-P, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS with 0.05% sodium azide
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
CD300F Antibody (YA4381) is a Mouse-derived and non-conjugated IgG2b monoclonal antibody, targeting to CD300F.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 32 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 32.3 kDa
Purified recombinant fragment of human CD300F (AA: Extra(20-156)) expressed in E. Coli.
affinity purified.
Non-conjugated
Unmodified
IgG2b
Product Properties
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Appearance
Liquid
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Formulation
Supplied in PBS with 0.05% sodium azide
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
CD300F acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis. Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands. May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2. Inhibits osteoclast formation. Induces macrophage cell death upon engagement[1][2][3].
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Subcellular Localization
Cell membrane; Single-pass type I membrane protein
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Expression
Tissue_specificity:Highly expressed in spleen, peripheral blood leukocyte and monocyte, and lung. Weakly expressed in thymus, heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, testis, ovary, small intestine or colon. Expressed selectively in monocytes and monocyte-related cells -
Isoforms & Post-Translational Modification
Q8TDQ1 has 6 isomers: Q8TDQ1-1: 32335 Da (predicted); Q8TDQ1-2: 26480 Da (predicted); Q8TDQ1-3: 18684 Da (predicted); Q8TDQ1-4: 21427 Da (predicted); Q8TDQ1-5: 26671 Da (predicted); Q8TDQ1-6: 32628 Da (predicted).
Phosphorylated on tyrosine -
Subunit
Interacts with PTPN6/SHP-1 in a tyrosine phosphorylation dependent manner (PubMed:15184070, PubMed:15549731). Interacts with IL4R (By similarity)
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SwissProt ID
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Synonyms
CLM1; NKIR; CLM-1; IREM1; LMIR3; CD300lf; IREM-1; IgSF13
Documentation
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