Galectin 1 Antibody (YA3725)(PBS only)
(Synonyms: GBP; GAL1)Galectin 1 Antibody (YA3725) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Galectin 1.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P, FC, ELISA
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Reactivity :
Human
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Formulation:
Supplied in PBS, pH 7.4.
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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FC
FC: Flow Cytometry
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ELISA
ELISA: Enzyme Linked Immunosorbent Assay
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|---|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:200-1:400 | 1:10000 |
Product Details
Galectin 1 Antibody (YA3725) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Galectin 1.
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Host Mouse
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Species ReactivityHuman
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Observed Molecular WeightObserved band size: 15 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
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Calculated Molecular Weight Predicted band size: 15 kDa
Purified recombinant fragment of human LGALS1 (AA: 1-135) expressed in E. Coli.
affinity purified.
Non-conjugated
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS, pH 7.4.
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
Galectin-1 is a β-galactoside-binding protein with intracellular and extracellular activity, and evidence identifies Galectin-1 and its ligands as regulators of T-cell homeostasis, survival, immune disorders, inflammation, allergies, and host-pathogen interactions[1]. Mechanistically, Galectin-1 regulates immune and inflammatory cells through carbohydrate-dependent binding and cross-linking of cell-surface glycoproteins, including CD2, CD3, CD7, CD43, and CD45 on T cells[1]. In disease models, Galectin-1 inhibited microglial activation and ameliorated neurodegeneration in a Parkinson’s disease model through the MAPK/IκB/NFκB axis and its carbohydrate-recognition domain[2]. In collagen-induced arthritis, absence of endogenous Galectin-1 increased susceptibility and disease severity, supporting its inhibitory role in experimental inflammation[3]. Compared with related isoforms, Galectin-1 and Galectin-3 bind distinct T-cell surface glycoprotein receptors, while Galectin-9 and Galectin-1 require different glycan ligands and glycoprotein receptors to trigger T-cell death[4][5]. For experimental oncology applications, Galectin-1 overexpression was detected in hepatocellular carcinoma, and OTX008 combined with sorafenib significantly reduced tumor growth and size in tumor-cell models[6].
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Subcellular Localization
Secreted, extracellular space, extracellular matrix; Cytoplasm; Secreted
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Expression
Tissue_specificity:It is expressed in the placenta, maternal decidua, and fetal membranes. In the placenta, it is expressed in trophoblast cells, mesenchymal cells, villous endothelial cells, syncytiotrophoblast apical membrane, and villous mesenchyme. In the fetal membranes, it is expressed in the amnion, chorion, amniotic mesenchyme, and chorion (protein level) . It is also expressed in cardiac muscle, smooth muscle, skeletal muscle, neurons, thymus, kidneys, and hematopoietic cells. -
Subunit
Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD6, CD7, CD43, ALCAM and CD45. Interacts with laminin (via poly-N-acetyllactosamine). Interacts with SUSD2. Interacts with cargo receptor TMED10; the interaction mediates the translocation from the cytoplasm into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and thereby secretion (PubMed:32272059). Interacts with CD69 (PubMed:24752896)
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SwissProt ID
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Synonyms
GBP; GAL1
Documentation
References
[1]. Camby I, et al. Galectin-1: a small protein with major functions. Glycobiology. 2006 Nov;16(11):137R-157R. [Content Brief]
[2]. Li Y, et al. Galectin-1 attenuates neurodegeneration in Parkinson's disease model by modulating microglial MAPK/IκB/NFκB axis through its carbohydrate-recognition domain. Brain Behav Immun. 2020 Jan;83:214-225. [Content Brief]
[3]. Iqbal AJ, et al. Endogenous galectin-1 exerts tonic inhibition on experimental arthritis. J Immunol. 2013 Jul 1;191(1):171-7. [Content Brief]
[4]. Stillman BN, et al. Galectin-3 and galectin-1 bind distinct cell surface glycoprotein receptors to induce T cell death. J Immunol. 2006 Jan 15;176(2):778-89. [Content Brief]
[5]. Bi S, et al. Structural features of galectin-9 and galectin-1 that determine distinct T cell death pathways. J Biol Chem. 2008 May 2;283(18):12248-58. [Content Brief]
[6]. Leung Z, et al. Galectin-1 promotes hepatocellular carcinoma and the combined therapeutic effect of OTX008 galectin-1 inhibitor and sorafenib in tumor cells. J Exp Clin Cancer Res. 2019 Oct 22;38(1):423. [Content Brief]
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