GCET2 Antibody (YA5959)

Cat. No.: HY-P86267: Data Sheet User Guide for Antibodies
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GCET2 Antibody (YA5959) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GCET2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

GCET2 Antibody (YA5959) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to GCET2.

Host

Rabbit

Clonality

Monoclonal

Molecular Weight

Observed band size: 20 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q8N6F7

Gene ID

257144 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:100-300
ICC	ICC/IF: 1:100-300
FC 1	100-300

Purity	Protein A	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation

Subcellular Localization：Cytoplasm; Cell membrane

Expression：
Tissue_specificity:It is expressed (protein level) in diffuse large B-cell lymphoma (DLBCL) and various germinal center (GC) -like lymphoma cell lines. It is highly expressed in normal GC lymphocytes and GC-derived malignancies. It is also expressed in the thymus and spleen.

Induction:Up-regulated by IL4/interleukin-4

Isoforms & Post-Translational Modification：Q8N6F7 has 3 isomers: Q8N6F7-1: 21005 Da (predicted); Q8N6F7-2: 21240 Da (predicted); Q8N6F7-3: 19206 Da (predicted).
Phosphorylation on tyrosine residues can be induced by IL6. Phosphorylation is mediated by LYN;Targeted by the ubiquitin E3 ligase subunit FBXO10 to mediate its ubiquitination and degradation

Subunit：Interacts with ACTB and MYH2; the interaction with MYH2 is increased by IL6-induced phosphorylation. Interacts (via C-terminus) with ARHGEF11 (via DH domain).

Synonyms

GCSAM; GAL; GCET2; Germinal center-associated signaling and motility protein ; Germinal center B-cell-expressed transcript 2 protein; Germinal center-associated lymphoma protein; hGAL;

Documentation

User Guide for Antibodies (1077 KB)