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HIF-2 alpha Antibody (YA4453)

(Synonyms: HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73)
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Based on 1 Customer Validation

HIF-2 alpha Antibody (YA4453) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HIF-2 alpha.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for HIF-2 alpha Antibody (YA4453)
  • Experimental Validation Results for HIF-2 alpha Antibody (YA4453)
  • HIF-2 alpha Antibody (YA4453) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

HIF-2 alpha Antibody (YA4453) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to HIF-2 alpha.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 96 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 96 kDa
Immunogen

Purified recombinant fragment of human HIF2a (AA: 680-870) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG1

RRID

AB_3718979

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for HIF-2 alpha Antibody (YA4453)
    Immunocytochemistry analysis of HepG2 cells labeling HIF-2 alpha with HIF-2 alpha Antibody (HY-P84756) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with HIF-2 alpha Antibody (HY-P84756) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for HIF-2 alpha Antibody (YA4453)
    Immunocytochemistry analysis of HepG2 cells labeling HIF-2 alpha with HIF-2 alpha Antibody (HY-P84756) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with HIF-2 alpha Antibody (HY-P84756) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    HIF-2 alpha transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD

  • Subcellular Localization

    Nucleus; Nucleus speckle

  • Expression


    Tissue_specificity:It is expressed in most tissues, with the highest expression levels in the placenta, lungs, and heart. It is selectively expressed in endothelial cells.

  • Subunit

    Interacts with HIF3A (By similarity). Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (PubMed:16181639). Interacts with CREBBP (By similarity). Interacts with EGLN1. Interacts with VHL (PubMed:19208626)

  • SwissProt ID

    Q99814

  • Gene ID
    2034 [NCBI]
  • Synonyms

    HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73

HIF-2 alpha Antibody (YA4453) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling HIF-2 alpha with HIF-2 alpha Antibody (HY-P84756) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with HIF-2 alpha Antibody (HY-P84756) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling HIF-2 alpha with HIF-2 alpha Antibody (HY-P84756) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with HIF-2 alpha Antibody (HY-P84756) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • HIF-2 alpha Antibody (YA4453) Product Image
    1/16
  • Experimental Validation Results for HIF-2 alpha Antibody (YA4453)
  • Experimental Validation Results for HIF-2 alpha Antibody (YA4453)
  • HIF-2 alpha Antibody (YA4453) Product Image
  • Experimental Validation Results for HIF-2 alpha Antibody (YA4453)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling HIF-2 alpha with HIF-2 alpha Antibody (HY-P84756) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with HIF-2 alpha Antibody (HY-P84756) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for HIF-2 alpha Antibody (YA4453)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of HepG2 cells labeling HIF-2 alpha with HIF-2 alpha Antibody (HY-P84756) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with HIF-2 alpha Antibody (HY-P84756) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for HIF-2 alpha Antibody (YA4453)
  • Experimental Validation Results for HIF-2 alpha Antibody (YA4453)
  • HIF-2 alpha Antibody (YA4453) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling HIF-2 alpha with HIF-2 alpha Antibody (HY-P84756) at 1/300 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with HIF-2 alpha Antibody (HY-P84756) at 1/300 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • HIF-2 alpha Antibody (YA4453) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of HepG2 cells labeling HIF-2 alpha with HIF-2 alpha Antibody (HY-P84756) at 1/600 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with HIF-2 alpha Antibody (HY-P84756) at 1/600 dilution in quick block buffer overnight at 4 ℃.AF488-conjugated Goat Anti-Mouse IgG H&L (HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • HIF-2 alpha Antibody (YA4453) Product Image ICC
  • HIF-2 alpha Antibody (YA4453) Product Image ICC