ID2 Antibody (YA4168)

Cat. No.: HY-P84471: Fiche technique User Guide for Antibodies
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ID2 Antibody (YA4168) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ID2.

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Size	Stock	Quantité
20 μL	Obtenir un devis 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
50 μL	Obtenir un devis 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
100 μL	Obtenir un devis 2 - 3 Weeks 1 - 2 Weeks 3 - 4 Weeks 2 - 3 weeks	Estimated Time of Arrival: December 31
250 μL	Obtenir un devis

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
Description
Références

Description

ID2 Antibody (YA4168) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ID2.

Host

Mouse

Clonality

Monoclonal

Masse moléculaire

Predicted band size: 15 kDa;

Observed band size: 15 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q02363

Gene ID

3398 [NCBI]

Immunogen

Purified recombinant fragment of human ID2 (AA: 1-134) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureté	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Solution

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Livraison

Shipping with blue ice.

Verification Image

Flow cytometric analysis of 1X10^6 Hela cells labeling ID2 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃. AF 488 Goat Anti-mouse IgG H&L was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

Background

Function：Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Restricts the CLOCK and BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:Highly expressed in early fetal tissues, including those of the central nervous system

Subunit：Interacts with GATA4 and NKX2-5 (By similarity). Interacts with NR0B2 (PubMed:14752053). Interacts with CLOCK and BMAL1 (PubMed:20861012). Interacts with IFI204 (By similarity). Interacts with NEDD9/HEF1 (PubMed:10502414). Interacts with ASB4; this interaction promotes ID2 proteasomal degradation (PubMed:24586788)

Synonyms

GIG8; ID2A; ID2H; bHLHb26

Documentation

User Guide for Antibodies (1077 KB)

Références

[1]. /biology-dictionary/a549-cell.html [Content Brief]