MRP4 Antibody (YA3530)(PBS only)

Cat. No.: HY-P83833A: Ficha de datos User Guide for Antibodies
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MRP4 Antibody (YA3530) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MRP4.

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Tamaño		Stock
50 μL		Obtener un presupuesto
100 μL		Obtener un presupuesto

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Descripciòn

Descripciòn

MRP4 Antibody (YA3530) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to MRP4.

Host

Mouse

Peso molecular

Predicted band size: 150 kDa;

Observed band size: 150 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

O15439

Gene ID

10257 [NCBI]

Immunogen

Purified recombinant fragment of human ABCC4 (AA: 631-692) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Pureza	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Background

Function：ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes physiological compounds and xenobiotics from cells. Transports a range of endogenous molecules that have a key role in cellular communication and signaling, including cyclic nucleotides such as cyclic AMP (cAMP) and cyclic GMP (cGMP), bile acids, steroid conjugates, urate, and prostaglandins (PubMed:11856762, PubMed:12523936, PubMed:12835412, PubMed:12883481, PubMed:15364914, PubMed:15454390, PubMed:16282361, PubMed:17959747, PubMed:18300232, PubMed:26721430). Mediates the ATP-dependent efflux of glutathione conjugates such as leukotriene C4 (LTC4) and leukotriene B4 (LTB4) too. The presence of GSH is necessary for the ATP-dependent transport of LTB4, whereas GSH is not required for the transport of LTC4 (PubMed:17959747). Mediates the cotransport of bile acids with reduced glutathione (GSH) (PubMed:12523936, PubMed:12883481, PubMed:16282361). Transports a wide range of drugs and their metabolites, including anticancer, antiviral and antibiotics molecules (PubMed:11856762, PubMed:12105214, PubMed:15454390, PubMed:17344354, PubMed:18300232). Confers resistance to anticancer agents such as methotrexate (PubMed:11106685)

Subcellular Localization：Basolateral cell membrane; Multi-pass membrane protein; Apical cell membrane; Multi-pass membrane protein

Expression：
Tissue_specificity:It is widely expressed, with particularly high levels in the prostate, but is almost undetectable in the liver. (Hepatocyte sinusoidal membrane)

Isoforms & Post-Translational Modification：O15439 has 4 isomers: O15439-1: 149527 Da (predicted); O15439-2: 144201 Da (predicted); O15439-3: 96841 Da (predicted); O15439-4: 88138 Da (predicted).
N-glycosylated; leading to substrate-selective effects on its transport activity

Subunit：Interacts (via PDZ-binding motif) with SNX27 (via PDZ domain); this interaction accelerates MRP4 internalization

Synonyms

MRP4; MOATB; MOAT-B; EST170205; ABCC 4 antibody; ABCC4 antibody; ATP binding cassette sub family C (CFTR/MRP) member 4 antibody; ATP binding cassette sub family C member 4 antibody

Documentación

User Guide for Antibodies (1077 KB)