NEK6 Antibody (YA9673)
(Synonyms: SID6-1512)NEK6 Antibody (YA9673) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to NEK6.
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Host:
Mouse
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Isotype:
IgG
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Application:
IHC-P, ICC/IF, FC
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Reactivity :
Human, Mouse, Rat
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Formulation:
Supplied in PBS (pH7.4).
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Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
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ICC/IF
ICC/IF: Immunocytochemistry/
Immunofluorescence |
FC
FC: Flow Cytometry
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|---|---|---|---|
| Dilution Ratio | 1:50 | 1:100 | 1:100 |
Product Details
NEK6 Antibody (YA9673) is a Mouse-derived and non-conjugated IgG1 Monoclonal antibody, targeting to NEK6.
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Host Mouse
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Clonality Monoclonal
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Species ReactivityHuman, Mouse, Rat
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Calculated Molecular Weight Predicted band size: 35.5 kDa;
Entrez Gene: 10783
Recombinant protein of human NEK6 (Full length) produced in HEK293T cell.
Endogenous
affinity purified.
Non-conjugated
Unmodified
IgG
Product Properties
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Appearance
Solution
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Formulation
Supplied in PBS (pH7.4).
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Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
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Shipping
Shipping with blue ice.
Background
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Function
NEK6 is a Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIRSR, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence. Phosphorylates EML4 at 'Ser-144', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression[1][2][3][4][5][6][7][8][9].
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Subcellular Localization
Cytoplasm; Nucleus; Nucleus speckle; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome; Cytoplasm, cytoskeleton, spindle pole
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Expression
Tissue_Specificity: Ubiquitous, with highest expression in heart and skeletal muscle.
Induction: Up-regulated during the M phase of cell cycle progression. Down-regulated in both replicative and premature senescence of cancer cells. -
Isoforms & Post-Translational Modification
NEK6 has 4 isoforms, Q9HC98-1: amino acid length is 313, molecular weight is 35714 Da (predicted); Q9HC98-2: amino acid length is 347, molecular weight is 39844 Da (predicted); Q9HC98-3: amino acid length is 331, molecular weight is 37823 Da (predicted); Q9HC98-4: amino acid length is 338, molecular weight is 38460 Da (predicted).
Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210 -
Subunit
Interacts with STAT3 and RPS6KB1 (By similarity).
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SwissProt ID
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Synonyms
SID6-1512
Documentation
References
[1]. Belham C, et al. Identification of the NIMA family kinases NEK6/7 as regulators of the p70 ribosomal S6 kinase. Curr Biol. 2001 Aug 7;11(15):1155-67. [Content Brief]
[2]. Yin MJ, et al. The serine/threonine kinase Nek6 is required for cell cycle progression through mitosis. J Biol Chem. 2003 Dec 26;278(52):52454-60. [Content Brief]
[3]. O'Regan L, et al. The Nek6 and Nek7 protein kinases are required for robust mitotic spindle formation and cytokinesis. Mol Cell Biol. 2009 Jul;29(14):3975-90. [Content Brief]
[4]. Hashimoto Y, et al. Identification and characterization of Nek6 protein kinase, a potential human homolog of NIMA histone H3 kinase. Biochem Biophys Res Commun. 2002 May 3;293(2):753-8. [Content Brief]
[5]. Vaz Meirelles G, et al. Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome. J Proteome Res. 2010 Dec 3;9(12):6298-316. [Content Brief]
[6]. Rapley J, et al. The NIMA-family kinase Nek6 phosphorylates the kinesin Eg5 at a novel site necessary for mitotic spindle formation. J Cell Sci. 2008 Dec 1;121(Pt 23):3912-21. [Content Brief]
[7]. Lee MY, et al. Nek6 is involved in G2/M phase cell cycle arrest through DNA damage-induced phosphorylation. Cell Cycle. 2008 Sep 1;7(17):2705-9. [Content Brief]
[8]. Jee HJ, et al. Nek6 overexpression antagonizes p53-induced senescence in human cancer cells. Cell Cycle. 2010 Dec 1;9(23):4703-10. [Content Brief]
[9]. Adib R, et al. Mitotic phosphorylation by NEK6 and NEK7 reduces the microtubule affinity of EML4 to promote chromosome congression. Sci Signal. 2019 Aug 13;12(594):. [Content Brief]
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