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Phospho-eIF4EBP1 (Ser65) Antibody (YA4245)

(Synonyms: EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I)
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Phospho-eIF4EBP1 (Ser65) Antibody (YA4245) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Phospho-eIF4EBP1 (Ser65).

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG1

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Phospho-eIF4EBP1 (Ser65) Antibody (YA4245) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:100-1:500 1:200-1:400 1:10000

Product Details

Description

Phospho-eIF4EBP1 (Ser65) Antibody (YA4245) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Phospho-eIF4EBP1 (Ser65).

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 15-20 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 13 kDa
Immunogen

Synthesized peptide of human Phospho-4E-BP1 (Ser65).(FLMESRNS(p)PVTKTP).

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Phosphorylated

Isotype

IgG1

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Background

  • Function

    eIF4EBP1 is a Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways

  • Subcellular Localization

    Cytoplasm; Nucleus

  • Subunit

    Hypophosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation (PubMed:12150926, PubMed:16271312, PubMed:17368478, PubMed:17631896, PubMed:22578813, PubMed:25702871, PubMed:7935836, PubMed:8521827).

  • SwissProt ID

    Q13541

  • Gene ID
    1978 [NCBI]
  • Synonyms

    EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Phospho-eIF4EBP1 (Ser65) Antibody (YA4245) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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  • Phospho-eIF4EBP1 (Ser65) Antibody (YA4245) Product Image