FRK Antibody (YA5039)
(Synonyms: FRK; PTK5; RAK; Tyrosine-protein kinase FRK; FYN-related kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5)FRK Antibody (YA5039) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FRK.
-
Host:
Mouse
-
Isotype:
IgG
-
Application:
IHC-P, ELISA
-
Reactivity :
Human
-
Formulation:
Supplied in Ascitic fluid containing 0.03% sodium azide.
-
Conjugation:
Non-conjugated
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
Applications
| Application |
IHC-P
IHC-P: Immunohistochemistry-Paraffin
|
ELISA
ELISA: Enzyme Linked Immunosorbent Assay
|
|---|---|---|
| Dilution Ratio | 1:200-1:1000 | 1:10000 |
Product Details
FRK Antibody (YA5039) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to FRK.
-
Host Mouse
-
Clonality Monoclonal
-
Species ReactivityHuman
-
Calculated Molecular Weight Predicted band size: 58 kDa;
Purified recombinant fragment of FRK (aa2-300) expressed in E. Coli. The exact sequence is proprietary to MCE.
Endogenous
affinity purified
Non-conjugated
Unmodified
IgG
Product Properties
-
Appearance
Solution
-
Formulation
Supplied in Ascitic fluid containing 0.03% sodium azide.
-
Storage & Stability
Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
-
Shipping
Shipping with blue ice.
Background
-
Function
FRK is a non-receptor tyrosine kinase in the BRK family kinases, distantly related to Src family kinases, and its biology centers on phosphorylation-dependent control of growth and signaling[1][2]. Mechanistically, FRK has been linked to PTEN stability, EGFR internalization, cyclin D1 nuclear accumulation, ITGB1/FAK signaling, and YAP ubiquitylation, placing it in cell-cycle, receptor-trafficking, adhesion, and Hippo-pathway research[3][4][5][6][7]. In disease models, FRK suppressed breast cancer cell proliferation and invasion, inhibited glioma growth or progression in several studies, but recurrent FRK-activating mutations drove STAT3 activation and proliferation in hepatocellular adenoma[3][5][6][7][8]. Compared with related isoforms, FRK/Rak/Gtk/Iyk/Bsk belongs to a conserved Brk/Srm/Frk/Src42A group that remains structurally distinct from c-Src and Fyn[2]. For experimental applications, FRK fusions in ALK-negative anaplastic large-cell lymphoma showed kinase targetability, and FRK-activating hepatocellular adenoma mutations showed sensitivity to Src inhibitors[8][9].
-
Subcellular Localization
Cytoplasm; Nucleus
-
Expression
Tissue_specificity:It is primarily expressed in epithelial cell lines and tissues, particularly in normal liver, kidney, mammary gland, and colon. -
Isoforms & Post-Translational Modification
P42685 has 2 isomers: P42685-1: 58254 Da (predicted); P42685-2: 41980 Da (predicted).
-
Subunit
Interacts (via the SH3-domain) with PTEN. Interacts with RB1
-
SwissProt ID
-
Synonyms
FRK; PTK5; RAK; Tyrosine-protein kinase FRK; FYN-related kinase; Nuclear tyrosine protein kinase RAK; Protein-tyrosine kinase 5
Documentation
References
[1]. Goel RK, et al. Understanding the cellular roles of Fyn-related kinase (FRK): implications in cancer biology. Cancer Metastasis Rev. 2016 Jun;35(2):179-99. [Content Brief]
[2]. Serfas MS, et al. Brk, Srm, Frk, and Src42A form a distinct family of intracellular Src-like tyrosine kinases. Oncol Res. 2003;13(6-10):409-19. [Content Brief]
[3]. Yim EK, et al. Rak functions as a tumor suppressor by regulating PTEN protein stability and function. Cancer Cell. 2009 Apr 7;15(4):304-14. [Content Brief]
[4]. Jin L, et al. The Rak/Frk tyrosine kinase associates with and internalizes the epidermal growth factor receptor. Oncogene. 2014 Jan 16;33(3):326-35. [Content Brief]
[5]. Hua L, et al. FRK suppresses the proliferation of human glioma cells by inhibiting cyclin D1 nuclear accumulation. J Neurooncol. 2014 Aug;119(1):49-58. [Content Brief]
[6]. Wang J, et al. FRK suppresses human glioma growth by inhibiting ITGB1/FAK signaling. Biochem Biophys Res Commun. 2019 Oct 1;517(4):588-595. [Content Brief]
[7]. Wang Y, et al. FRK inhibits glioblastoma progression via phosphorylating YAP and inducing its ubiquitylation and degradation by Siah1. Neuro Oncol. 2022 Dec 1;24(12):2107-2120. [Content Brief]
[8]. Pilati C, et al. Genomic profiling of hepatocellular adenomas reveals recurrent FRK-activating mutations and the mechanisms of malignant transformation. Cancer Cell. 2014 Apr 14;25(4):428-41. [Content Brief]
[9]. Hu G, et al. Targetable fusions of the FRK tyrosine kinase in ALK-negative anaplastic large cell lymphoma. Leukemia. 2018 Feb;32(2):565-569. [Content Brief]
Powered by Bioz
