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ACRV1 Antibody (YA3916)

(Synonyms: ACRV1,SP-10; SPACA2; D11S4365)
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Based on 1 Customer Validation

ACRV1 Antibody (YA3916) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ACRV1.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG

  • Application:

    IHC-P, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for ACRV1 Antibody (YA3916)
  • Experimental Validation Results for ACRV1 Antibody (YA3916)
  • ACRV1 Antibody (YA3916) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

ACRV1 Antibody (YA3916) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to ACRV1.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 14-36 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 28 kDa
Immunogen

Purified recombinant fragment of human SP10 (AA: 22-265) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for ACRV1 Antibody (YA3916)
    Flow cytometric analysis of 1X10^6 Hela cells labeling SP10 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for ACRV1 Antibody (YA3916)
    Flow cytometric analysis of 1X10^6 SK-OV-3 cells labeling SP10 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

Background

  • Function

    ACRV1 (SP-10) is an abundant protein found in the acrosome matrix and inner acrosome membrane. ACRV1 antibodies have been shown to be used for spermatogenesis cycle staging in mice and humans.

  • Subcellular Localization

    Cytoplasmic vesicle, secretory vesicle, acrosome

  • Expression


    Tissue_specificity:testis

  • Isoforms & Post-Translational Modification

    ACRV1 has 11 isomers: P26436-1: 28156 Da (predicted); P26436-2: 26401 Da (predicted); P26436-3: 22375 Da (predicted); P26436-4: 20858 Da (predicted); P26436-5: 19102 Da (predicted); P26436-6: 18267 Da (predicted); P26436-7: 17763 Da (predicted); P26436-8: 16368 Da (predicted); P26436-9: 16272 Da (predicted); P26436-10: 13520 Da (predicted); P26436-11: 9030 Da (predicted).

  • SwissProt ID

    P26436

  • Gene ID
    56 [NCBI]
  • Synonyms

    ACRV1,SP-10; SPACA2; D11S4365

ACRV1 Antibody (YA3916) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling SP10 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • 1/16

    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 SK-OV-3 cells labeling SP10 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • ACRV1 Antibody (YA3916) Product Image
    1/16
  • Experimental Validation Results for ACRV1 Antibody (YA3916)
  • Experimental Validation Results for ACRV1 Antibody (YA3916)
  • ACRV1 Antibody (YA3916) Product Image
  • Experimental Validation Results for ACRV1 Antibody (YA3916)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 Hela cells labeling SP10 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for ACRV1 Antibody (YA3916)
    1/1
    MedChemExpress Validation
     Flow cytometric analysis of 1X10^6 SK-OV-3 cells labeling SP10 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).
  • Experimental Validation Results for ACRV1 Antibody (YA3916)
  • Experimental Validation Results for ACRV1 Antibody (YA3916)
  • ACRV1 Antibody (YA3916) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 Hela cells labeling SP10 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • ACRV1 Antibody (YA3916) Product Image
    MedChemExpress Validation

    Flow cytometric analysis of 1X10^6 SK-OV-3 cells labeling SP10 Antibody(red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Then stained with the primary antibody at 1/400 dilution overnight at 4℃.Alexa Fluor® 488 Goat Anti-mouse IgG H&L (invitrogen A11001) was used as the secondary antibody at 1/1,000 dilution for 45 minutes at room temperature. Mouse IgG Isotype Control (Invitrogen 14-4714-B2, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (gray).

  • ACRV1 Antibody (YA3916) Product Image FC
  • ACRV1 Antibody (YA3916) Product Image FC