TIGIT Antibody (YA1497)

製品番号: HY-P81752: データシート SDS COA User Guide for Antibodies
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TIGIT Antibody (YA1497) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIGIT.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$216	在庫あり	Estimated Time of Arrival: December 31
100 μL	$342	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
説明

製品説明

TIGIT Antibody (YA1497) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIGIT.

Host

Rabbit

Clonality

Recombinant, Monoclonal

分子量

Predicted band size: 26.3 kDa;

Observed band size: 25 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q495A1

Gene ID

201633 [NCBI]

Immunogen

Recombinant human TIGIT protein

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:50-1:100
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	純度	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Background

Function：Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed:19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed:23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed:24817116). Also acts as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed:32503945)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:Expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level)

Isoforms & Post-Translational Modification：Q495A1 has 2 isomers: Q495A1-1: 26319 Da (predicted); Q495A1-2: 18432 Da (predicted).

Subunit：Homodimer in cis; binds with high affinity to PVR, forming a heterotetrameric assembly of two TIGIT and two PVR molecules. Binds with lower affinity to NECTIN2 and NECTIN3. Interacts with GRB2 (PubMed:23154388). Interacts with NECTIN4 (PubMed:32503945)

RRID

AB_3103858

Database

Entrez Gene: 201633 Human

SwissProt: Q495A1 Human

OMIM: 612859 Human

Research Field

Immunology

Synonyms

TIGIT; VSIG9; VSTM3

ドキュメンテーション

Select Batch:

データシート (232 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)