TIGIT Antibody (YA1497)

Cat. No.: HY-P81752: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

TIGIT Antibody (YA1497) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIGIT.

For research use only. We do not sell to patients.

Size	Stock	Quantity
Free Sample	Apply now
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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TIGIT Antibody (YA1497) Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TIGIT Antibody (YA1497) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to TIGIT.

Host

Rabbit

Clonality

Recombinant, Monoclonal

Molecular Weight

Predicted band size: 26.3 kDa;

Observed band size: 25 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q495A1

Gene ID

201633 [NCBI]

Immunogen

Recombinant human TIGIT protein

Application &
Dilution Ratio

Application	Dilution Ratio
FC FC: Flow Cytometry	1:50-1:100
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Sensitivity	Endogenous	Purity	Affinity Chromatography
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Inhibitory receptor that plays a role in the modulation of immune responses. Suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells (PubMed:19011627). Upon binding to its ligands PVR/CD155 or NECTIN2/CD112, which are expressed on antigen-presenting cells, sends inhibitory signals to the T-cell or NK cell. Mechanistically, interaction with ligand leads to phosphorylation of the cytoplasmic tail by Src family tyrosine kinases such as FYN or LCK, allowing subsequent binding to adapter GRB2 and SHIP1/INPP5D. In turn, inhibits PI3K and MAPK signaling cascades (PubMed:23154388). In addition, associates with beta-arrestin-2/ARRB2 to recruit SHIP1/INPP5D that suppresses autoubiquitination of TRAF6 and subsequently inhibits NF-kappa-B signaling pathway (PubMed:24817116). Also acts as a receptor for NECTIN4 to inhibit NK cell cytotoxicity (PubMed:32503945)

Subcellular Localization：Cell membrane; Single-pass type I membrane protein

Expression：
Tissue_specificity:Expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level)

Isoforms & Post-Translational Modification：Q495A1 has 2 isomers: Q495A1-1: 26319 Da (predicted); Q495A1-2: 18432 Da (predicted).

Subunit：Homodimer in cis; binds with high affinity to PVR, forming a heterotetrameric assembly of two TIGIT and two PVR molecules. Binds with lower affinity to NECTIN2 and NECTIN3. Interacts with GRB2 (PubMed:23154388). Interacts with NECTIN4 (PubMed:32503945)

RRID

AB_3103858

Database

Entrez Gene: 201633 Human

SwissProt: Q495A1 Human

OMIM: 612859 Human

Research Field

Immunology

Synonyms

TIGIT; VSIG9; VSTM3

Documentation

Select Batch:

Data Sheet (232 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

TIGIT Antibody (YA1497) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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TIGIT Antibody (YA1497)

TIGIT Antibody (YA1497) Featured Recommendations:

Product Detail

Background

Documentation

TIGIT Antibody (YA1497) Related Classifications

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