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Toll-Like Receptor 9 Antibody (YA4520)

(Synonyms: CD289)
Customer Review

Based on 1 Customer Validation

Toll-Like Receptor 9 Antibody (YA4520) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Toll-Like Receptor 9.

For research use only. We do not sell to patients.
  • Host:

    Mouse

  • Isotype:

    IgG2a

  • Application:

    IHC-P, ICC/IF, FC, ELISA

  • Reactivity :

    Human

  • Formulation:

    Supplied in PBS with 0.05% sodium azide

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA4520)
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA4520)
  • Toll-Like Receptor 9 Antibody (YA4520) Product Image
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Use of MCE Products

Applications

Application
IHC-P Info
IHC-P: Immunohistochemistry-Paraffin
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
FC Info
FC: Flow Cytometry
ELISA Info
ELISA: Enzyme Linked Immunosorbent Assay
Dilution Ratio 1:200-1:1000 1:200-1:1000 1:200-1:400 1:10000

Product Details

Description

Toll-Like Receptor 9 Antibody (YA4520) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Toll-Like Receptor 9.

  • Host Mouse
  • Clonality Monoclonal
  • Species Reactivity
    Human
  • Observed Molecular Weight
    Observed band size: 130 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 116 kDa
Immunogen

Purified recombinant fragment of human TLR9 (AA: 868-1016) expressed in E. Coli.

Purification

affinity purified.

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG2a

RRID

AB_3718989

Product Properties

  • Appearance

    Liquid

  • Formulation

    Supplied in PBS with 0.05% sodium azide

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA4520)
    Immunocytochemistry analysis of A549 cells labeling Toll-Like Receptor 9 with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA4520)
    Immunocytochemistry analysis of MCF-7 cells labeling Toll-Like Receptor 9 with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

  • Function

    Toll-Like Receptor 9 is key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production[1][2][3][4].

  • Subcellular Localization

    Endoplasmic reticulum membrane; Single-pass type I membrane protein; Early endosome membrane; Lysosome; Cytoplasmic vesicle, phagosome; Golgi apparatus membrane

  • Expression


    Tissue_specificity:This gene is highly expressed in the spleen, lymph nodes, tonsils, and peripheral blood leukocytes, especially in plasmacytoid anterior dendritic cells. Expression levels are much lower in monocytes and CD11c+ immature dendritic cells. It has also been detected in the lungs and liver.

  • Isoforms & Post-Translational Modification

    Q9NR96 has 5 isomers: Q9NR96-1: 115860 Da (predicted); Q9NR96-2: 109628 Da (predicted); Q9NR96-3: 118517 Da (predicted); Q9NR96-4: 118652 Da (predicted); Q9NR96-5: 114237 Da (predicted).
    Activated by proteolytic cleavage of the flexible loop between repeats LRR14 and LRR15 within the ectodomain. Cleavage requires UNC93B1. Proteolytically processed by first removing the majority of the ectodomain by either asparagine endopeptidase (AEP) or a cathepsin followed by a trimming event that is solely cathepsin mediated and required for optimal receptor signaling;Palmitoylated by ZDHHC3 in the Golgi regulates TLR9 trafficking from the Golgi to endosomes. Depalmitoylation by PPT1 controls the release of TLR9 from UNC93B1 in endosomes

  • Subunit

    Monomer and homodimer. Exists as a monomer in the absence of unmethylated cytidine-phosphate-guanosine (CpG) ligand.

  • SwissProt ID

    Q9NR96

  • Gene ID
    54106 [NCBI]
  • Synonyms

    CD289

Toll-Like Receptor 9 Antibody (YA4520) Related Classifications

MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Toll-Like Receptor 9 with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • 1/16

    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Toll-Like Receptor 9 with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Toll-Like Receptor 9 Antibody (YA4520) Product Image
    1/16
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA4520)
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA4520)
  • Toll-Like Receptor 9 Antibody (YA4520) Product Image
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA4520)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of A549 cells labeling Toll-Like Receptor 9 with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA4520)
    1/1
    MedChemExpress Validation
     Immunocytochemistry analysis of MCF-7 cells labeling Toll-Like Receptor 9 with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA4520)
  • Experimental Validation Results for Toll-Like Receptor 9 Antibody (YA4520)
  • Toll-Like Receptor 9 Antibody (YA4520) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of A549 cells labeling Toll-Like Receptor 9 with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Toll-Like Receptor 9 Antibody (YA4520) Product Image
    MedChemExpress Validation

    Immunocytochemistry analysis of MCF-7 cells labeling Toll-Like Receptor 9 with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with quick block buffer for 10 minutes at room temperature. Cells were then incubated with Toll-Like Receptor 9 Antibody (HY-P84823) at 1/200 dilution in quick block buffer overnight at 4 ℃. AF488-conjugated Goat Anti-Mouse IgG H&L（HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Toll-Like Receptor 9 Antibody (YA4520) Product Image ICC
  • Toll-Like Receptor 9 Antibody (YA4520) Product Image ICC