Toll-Like Receptor 9 Antibody (YA4520)

Cat. No.: HY-P84823: Data Sheet COA
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Toll-Like Receptor 9 Antibody (YA4520) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Toll-Like Receptor 9.

For research use only. We do not sell to patients.

Size	Stock	Quantity
10 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Toll-Like Receptor 9 Antibody (YA4520) is a Mouse-derived and non-conjugated IgG2a monoclonal antibody, targeting to Toll-Like Receptor 9.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 116 kDa;

Observed band size: 130 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q9NR96

Gene ID

54106 [NCBI]

Immunogen

Purified recombinant fragment of human TLR9 (AA: 868-1016) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG2a

Appearance

Liquid

Formulation

Supplied in PBS with 0.05% sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides (PubMed:14716310). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response (PubMed:11564765, PubMed:17932028). Controls lymphocyte response to Helicobacter infection (By similarity). Upon CpG stimulation, induces B-cell proliferation, activation, survival and antibody production (PubMed:23857366)

Subcellular Localization：Endoplasmic reticulum membrane; Single-pass type I membrane protein; Early endosome membrane; Lysosome; Cytoplasmic vesicle, phagosome; Golgi apparatus membrane

Expression：
Tissue_specificity:This gene is highly expressed in the spleen, lymph nodes, tonsils, and peripheral blood leukocytes, especially in plasmacytoid anterior dendritic cells. Expression levels are much lower in monocytes and CD11c+ immature dendritic cells. It has also been detected in the lungs and liver.

Isoforms & Post-Translational Modification：Q9NR96 has 5 isomers: Q9NR96-1: 115860 Da (predicted); Q9NR96-2: 109628 Da (predicted); Q9NR96-3: 118517 Da (predicted); Q9NR96-4: 118652 Da (predicted); Q9NR96-5: 114237 Da (predicted).
Activated by proteolytic cleavage of the flexible loop between repeats LRR14 and LRR15 within the ectodomain. Cleavage requires UNC93B1. Proteolytically processed by first removing the majority of the ectodomain by either asparagine endopeptidase (AEP) or a cathepsin followed by a trimming event that is solely cathepsin mediated and required for optimal receptor signaling;Palmitoylated by ZDHHC3 in the Golgi regulates TLR9 trafficking from the Golgi to endosomes. Depalmitoylation by PPT1 controls the release of TLR9 from UNC93B1 in endosomes

Subunit：Monomer and homodimer. Exists as a monomer in the absence of unmethylated cytidine-phosphate-guanosine (CpG) ligand.

RRID

AB_3718989

Synonyms

CD289

Documentation

Select Batch:

Data Sheet (235 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)