azo-Q2a 

azo-Q2a is a photoswitchable Na_V1.5 inhibitor. azo-Q2a exists as the trans isomer with low activity in the dark or under 480 nm illumination, and exists as the cis isomer with higher inhibitory potency under 365 nm illumination. azo-Q2a reduces the heart rate of living zebrafish larvae in a light-dependent manner. azo-Q2a can be used for the study of arrhythmias^[1].

Azo-Q2a (10 μM; during patch-clamp recordings) enables reversible, light-dependent inhibition of Na_V1.5 channels in HEK293T cells, with cis-azo-Q2a formed under 365 nm illumination showing 7-fold higher potency, with an IC₅₀ of 11.76 μM, than trans-azo-Q2a from dark/480 nm illumination, which has an IC₅₀ of 81.58 μM, without altering channel activation^[1].
Azo-Q2a (10 μM; during patch-clamp recordings) exhibits high light-dependent selectivity for Na_V1.5 over other Na_V subtypes and cardiac ion channels in HEK293T and CHO cells, with cis-azo-Q2a formed under 365 nm illumination showing significantly reduced hERG inhibition relative to quinidine^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Azo-Q2a (10 μM; soaked; dark incubation for 1-1.5 h) enables reversible optical regulation of heart rate in zebrafish larvae: under 365 nm illumination, the heart rate decreases to 56.5% of the basal level, and recovers to the basal level under 480 nm illumination^[1].
Azo-Q2a (10 μM; administered after tachycardia induction; 365 nm light irradiation for 5 min (cis isomer); dark incubation (trans isomer)) effectively terminates isoproterenol-induced tachycardia in zebrafish larvae when activated to its cis isomer by 365 nm light, reducing the heart rate to 35.3% of the baseline level, whereas the trans isomer in dark conditions exerts only a very weak effect^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

504.62

C₃₂H₃₂N₄O₂

COC1=CC2=C(N=CC(C3=CC=C(C=C3)/N=N/C4=CC=CC=C4)=C2[C@@H]([C@@H]5[N@](C[C@@H]6C=C)CC[C@H]6C5)O)C=C1

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Liu S, et al. Optical control of the cardiac rhythm with photoswitchable Na_V1.5 channel blockers. Nat Commun. Published online March 10, 2026.  [Content Brief]