BODIPY-Cyclopamine

Name: BODIPY-Cyclopamine
Brand: MedChemExpress
SKU: HY-137115
Rating: 4.5 (0 reviews)

Cat. No.: HY-137115 Pureza: 98.91%: Ficha de datos
COA

SDS
Instrucciones de manejo
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BODIPY-Cyclopamine is a fluorescently labeled ligand for the Smoothened (SMO) receptor. The activation of SMO is regulated by Patch protein, and over-activated SMO signaling pathways are associated with tumorigenesis. The NanoBRET (Nanofluorescein bioluminescence resonance energy transfer) technique used in the study can sensitively detect the resonance energy transfer between SMO and BODIPY-Cyclopamine, which can be used for high-throughput screening and kinetic analysis. Studying the binding site of BODIPY-Cyclopamine on SMO can also further explore SMO-targeted drugs.

Para uso exclusivo en investigación. No vendemos a pacientes.

BODIPY-Cyclopamine Estructura química

No. CAS : 334658-24-1

Tamaño	Stock	Cantidad
250 μg	En stock	Estimated Time of Arrival: December 31
500 μg	En stock	Estimated Time of Arrival: December 31
1 mg	En stock	Estimated Time of Arrival: December 31
5 mg	Obtener un presupuesto
10 mg	Obtener un presupuesto

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Revisión del cliente

Based on 1 Customer Validation

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Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

Peso molecular

841.92

Fòrmula

C₄₉H₇₀BF₂N₅O₄

No. CAS

334658-24-1

Appearance

Solid

Color

Orange to red

SMILES

C[C@H]1[C@@]2(O[C@@](C[C@@H]3C)([H])[C@@]1([H])N(C3)CCNC(CCCCCNC(CCC4=CC=C(C=C(C(C)=C5)[N]6=C5C)[N-]4[B+3]6([F-])[F-])=O)=O)C(C)=C(C[C@@]7([H])[C@@]8([H])CC=C(C9)[C@@]7(CC[C@@H]9O)C)[C@@]8([H])CC2

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (118.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1878 mL	5.9388 mL	11.8776 mL
5 mM	0.2376 mL	1.1878 mL	2.3755 mL
10 mM	0.1188 mL	0.5939 mL	1.1878 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (2.97 mM); Suspended solution

This protocol yields a suspended solution of ≥ 2.5 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Pureza y Documentación

Purity: 98.91%

Select Batch:

Ficha de datos (269 KB) SDS (252 KB)

COA (248 KB) HNMR (150 KB) RP-HPLC (246 KB) LCMS (235 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. A NanoBRET-Based Binding Assay for Smoothened Allows Real-time Analysis of Ligand Binding and Distinction of Two Binding Sites for BODIPY-cyclopamine

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1878 mL	5.9388 mL	11.8776 mL	29.6940 mL
	5 mM	0.2376 mL	1.1878 mL	2.3755 mL	5.9388 mL
	10 mM	0.1188 mL	0.5939 mL	1.1878 mL	2.9694 mL
	15 mM	0.0792 mL	0.3959 mL	0.7918 mL	1.9796 mL
	20 mM	0.0594 mL	0.2969 mL	0.5939 mL	1.4847 mL
	25 mM	0.0475 mL	0.2376 mL	0.4751 mL	1.1878 mL
	30 mM	0.0396 mL	0.1980 mL	0.3959 mL	0.9898 mL
	40 mM	0.0297 mL	0.1485 mL	0.2969 mL	0.7424 mL
	50 mM	0.0238 mL	0.1188 mL	0.2376 mL	0.5939 mL
	60 mM	0.0198 mL	0.0990 mL	0.1980 mL	0.4949 mL
	80 mM	0.0148 mL	0.0742 mL	0.1485 mL	0.3712 mL
	100 mM	0.0119 mL	0.0594 mL	0.1188 mL	0.2969 mL