1. Epigenetics
    TGF-beta/Smad
    Anti-infection
  2. PKC
    HIV
  3. Bryostatin 1

Bryostatin 1 

Cat. No.: HY-105231
Handling Instructions

Bryostatin 1 is a natural macrolide isolated from the bryozoan Bugula neritina and is a potent and central nervous system (CNS)-permeable PKC modulator. Bryostatin 1 binds to the isolated C1 domain of Munc13-1 and the full-length Munc13-1 protein with Kis of 8.07 nM and 0.45 nM, respectively. Bryostatin 1 has anti-cancer, anti-inflammatory, neuroprotective, anti-HIV-1 infection properties.

For research use only. We do not sell to patients.

Bryostatin 1 Chemical Structure

Bryostatin 1 Chemical Structure

CAS No. : 83314-01-6

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Description

Bryostatin 1 is a natural macrolide isolated from the bryozoan Bugula neritina and is a potent and central nervous system (CNS)-permeable PKC modulator. Bryostatin 1 binds to the isolated C1 domain of Munc13-1 and the full-length Munc13-1 protein with Kis of 8.07 nM and 0.45 nM, respectively. Bryostatin 1 has anti-cancer, anti-inflammatory, neuroprotective, anti-HIV-1 infection properties[1][2][3][4].

IC50 & Target

PKC[1]
HIV-1[4]

In Vitro

Bryostatin 1 (1 µM; 5 minutes; HT22 cells) treatment successfully recruits Munc13-1 from the cytosol to the plasma membrane. Effects of Bryostatin 1 on the other Munc13 family members, ubMunc13-2 and bMunc13-2, resembled those of Munc13-1 for translocation [1].
The increased level of expression of Munc13-1 following a 24 h incubation with Bryostatin 1 in both HT22 and primary mouse hippocampal cells is observed[1].
Bryostatin 1 can also affect the immune system by modulating dendritic cells (DCs) via toll-like receptor 4 (TLR4) through the MyD88-independent pathway, which favors an anti-inflammatory environment by inducing a type 2 phenotype that promotes the differentiation of CD4+ T-helper (Th) lymphocytes into Th2 versus Th1 effector cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HT22 cells
Concentration: 1 µM
Incubation Time: 5 minutes
Result: Caused Munc13-1 to transfer to the membrane fraction.
In Vivo

Bryostatin 1 (30 μg/kg; intraperitoneal injection; 3 d per week; for 2 weeks; C57BL/6J mice) treatment abolishes the onset of EAE[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female C57BL/6J mice (8-12-week-old) with MOG35-55[2]
Dosage: 30 μg/kg
Administration: Intraperitoneal injection; 3 d per week; for 2 weeks
Result: Abolished the onset of experimental autoimmune encephalomyelitis (EAE).
Clinical Trial
Molecular Weight

905.03

Formula

C₄₇H₆₈O₁₇

CAS No.

83314-01-6

SMILES

O[[email protected]]12[[email protected]](/C(C[[email protected]](C[[email protected]](OC(C[[email protected]@H](C[[email protected]@]3([H])O[[email protected]](O)(C(C)([[email protected]@H](OC(C)=O)C3)C)C[[email protected]@](C/4)([H])O[[email protected]@](CC4=C/C(OC)=O)([H])/C=C/C2(C)C)O)=O)([H])[[email protected]](O)C)([H])O1)=C/C(OC)=O)OC(/C=C/C=C/CCC)=O

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Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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Keywords:

Bryostatin 1Bryostatin1Bryostatin-1PKCHIVProtein kinase CHuman immunodeficiency virusMacrolideanti-cancerneuroprotectiveHIV-1Munc13-1translocationanti-inflammatoryCNSTLR4Inhibitorinhibitorinhibit

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Bryostatin 1
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