68654-25-1
Chemical Structure
Dibromobimane
- CAS No.: 68654-25-1
- Formula:C10H10Br2N2O2
- Molecular Weight:350.01
IUPAC Name: 8-((2R,3S)-5,7-dihydroxy-2-(4-hydroxyphenyl)-4-oxochroman-3-yl)-2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one
InChIKey: OSIYFMVMZXJKSP-UHFFFAOYSA-N
SMILES: O=C1C(C)=C(CBr)N2C(CBr)=C(C)C(N12)=O
Biological Activity: Dibromobimane is a Fluorescent dye and probe that can be used for the detection of intracellular reduced glutathione and the analysis of spatial proximity of closely adjacent cysteine thiol groups. Dibromobimane activates fluorescence through binding to thiol groups; its excitation/emission wavelengths are Ex/Em = 393/477 nm for glutathione detection, and Ex/Em = 385/477 nm for cysteine thiol cross-linking. Dibromobimane uses two equivalent bromomethyl groups to cross-link cysteine thiolates with a distance ranging from 3-6 Å. Dibromobimane can be applied to 96-well microplate-based high-throughput assays for labeling intracellular glutathione, and its fluorescence signal is normalized against nuclear staining results to eliminate the impact of cell density[1][2].
| Cat. No. | Product Name | Purity | Description | Pricing | |||||||||||||||||||
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Dibromobimane | 98.23% | Dibromobimane is a Fluorescent dye and probe that can be used for the detection of intracellular reduced glutathione and the analysis of spatial proximity of closely adjacent cysteine thiol groups. Dibromobimane activates fluorescence through binding to thiol groups; its excitation/emission wavelengths are Ex/Em = 393/477 nm for glutathione detection, and Ex/Em = 385/477 nm for cysteine thiol cross-linking. Dibromobimane uses two equivalent bromomethyl groups to cross-link cysteine thiolates with a distance ranging from 3-6 Å. Dibromobimane can be applied to 96-well microplate-based high-throughput assays for labeling intracellular glutathione, and its fluorescence signal is normalized against nuclear staining results to eliminate the impact of cell density. | ||||||||||||||||||||
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- [1]. Cox DP, et al. High Throughput Method for Assessment of Cellular Reduced Glutathione in Mammalian Cells. Journal of environmental protection science. 2007 Jan 01;1:23-28.
- [2]. Liang HP, et al. Redox properties of the tissue factor Cys186-Cys209 disulfide bond. The Biochemical journal. 2011 Aug 01;437(3):455-60.
Keywords
