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  3. Fluorescent Dye
  4. Dibromobimane

Dibromobimane is a thiol-selective fluorescent imaging agent. Dibromobimane is used to crosslink cysteine- and homocysteine-containing peptides.

For research use only. We do not sell to patients.

Dibromobimane

Dibromobimane Chemical Structure

CAS No. : 68654-25-1

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Based on 1 publication(s) in Google Scholar

Dibromobimane Related Antibodies

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Description

Dibromobimane is a thiol-selective fluorescent imaging agent. Dibromobimane is used to crosslink cysteine- and homocysteine-containing peptides[1].

Molecular Weight

350.01

Formula

C10H10Br2N2O2
CAS No.
Appearance

Solid

Color

White to light yellow

SMILES

O=C1C(C)=C(CBr)N2C(CBr)=C(C)C(N12)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (285.71 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8571 mL 14.2853 mL 28.5706 mL
5 mM 0.5714 mL 2.8571 mL 5.7141 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8571 mL 14.2853 mL 28.5706 mL 71.4265 mL
5 mM 0.5714 mL 2.8571 mL 5.7141 mL 14.2853 mL
10 mM 0.2857 mL 1.4285 mL 2.8571 mL 7.1427 mL
15 mM 0.1905 mL 0.9524 mL 1.9047 mL 4.7618 mL
20 mM 0.1429 mL 0.7143 mL 1.4285 mL 3.5713 mL
25 mM 0.1143 mL 0.5714 mL 1.1428 mL 2.8571 mL
30 mM 0.0952 mL 0.4762 mL 0.9524 mL 2.3809 mL
40 mM 0.0714 mL 0.3571 mL 0.7143 mL 1.7857 mL
50 mM 0.0571 mL 0.2857 mL 0.5714 mL 1.4285 mL
60 mM 0.0476 mL 0.2381 mL 0.4762 mL 1.1904 mL
80 mM 0.0357 mL 0.1786 mL 0.3571 mL 0.8928 mL
100 mM 0.0286 mL 0.1429 mL 0.2857 mL 0.7143 mL
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Dibromobimane Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dibromobimane68654-25-1Fluorescent DyeInhibitorinhibitorinhibit

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