Art. -Nr.: HY-121969

DDX3-IN-2

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DDX3-IN-2 is an active DEADbox polypeptide 3 (DDX3) inhibitor with an IC₅₀ value of 0.3 μM. DDX3-IN-2 shows a broad spectrum of antiviral activity. DDX3-IN-2 has the potential to overcome HIV resistance.

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Reinheit: 99.54%
CAS. Nr.: 1919828-81-1
Formel: C₂₀H₂₃N₅O
Molecular Weight:349.43

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

DDX3-IN-2 Chemical Structure

CAS. Nr.: 1919828-81-1

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

HIV

Alle HIV Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
BHK-21	CC₅₀	>50 μM Compound: 59	Cytotoxicity against hamster BHK-21 cells assessed as reduction in cell viability at MOI of 0.01 incubated for 48 hrs by plaque formation assay Cytotoxicity against hamster BHK-21 cells assessed as reduction in cell viability at MOI of 0.01 incubated for 48 hrs by plaque formation assay	[PMID: 35899912]
Huh-7	CC₅₀	>200 μM Compound: 1	Cytotoxicity against human HuH7 cells after 3 days by MTT assay Cytotoxicity against human HuH7 cells after 3 days by MTT assay	[PMID: 30721061]
Huh-7	IC₅₀	2.55 μM Compound: 1	Antiviral activity against Dengue virus serotype 2 New Guinea C infected at 50 TCID50 in human HuH7 cells assessed as inhibition of viral replication and 72 hrs later re-infected pre-seeded HuH7 cells with viral supernatant collected from previous infecte Antiviral activity against Dengue virus serotype 2 New Guinea C infected at 50 TCID50 in human HuH7 cells assessed as inhibition of viral replication and 72 hrs later re-infected pre-seeded HuH7 cells with viral supernatant collected from previous infecte	[PMID: 32435411]
PBMC	CC₅₀	>100 μM Compound: 59	Cytotoxicity against PBMC (unknown origin) assessed as reduction in cell viability Cytotoxicity against PBMC (unknown origin) assessed as reduction in cell viability	[PMID: 35899912]
PBMC	EC₅₀	0.97 μM Compound: 91	Antiviral activity against HIV1 TC-1 infected in human PBMC assessed as inhibition of viral replication Antiviral activity against HIV1 TC-1 infected in human PBMC assessed as inhibition of viral replication	[PMID: 38160620]

In Vitro

DDX3-IN-2 behaves as a competitive inhibitor with respect to the RNA substrate, which can be seen by the decrease in its inhibition potency as a function of increasing RNA substrate concentrations. DDX3-IN-2 is found to be completely inactive against the ATPase of DDX3, DDX1 helicase, and DENV NS3 helicase^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

DDX3-IN-2 (20 mg/kg; tail vein injection) possesses excellent biocompatibility, and Wistar rats shows a good tolerance to the dose of 20 mg/kg^[1].
DDX3-IN-2 (10 mg/kg; i.v. bolus injection; 0~25 hours) rapidly eliminates the half-life elimination and the plasmatic clearance values^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Wistar rats^[1]
Dosage:

Tail vein injection
Administration:

20 mg/kg
Result:

Possessed excellent biocompatibility, and Wistar rats showed a good tolerance to the dose of 20 mg/kg.

Animal Model:

Rats^[1]
Dosage:

I.v. bolus injection (Pharmacokinetic Analysis)
Administration:

10 mg/kg; 0~25 hours
Result:

Rapidly eliminated the half-life elimination and the plasmatic clearance values.

Chemical Information

CAS. Nr. 1919828-81-1
Appearance Solid
Molecular Weight 349.43
Formel C₂₀H₂₃N₅O
Color Off-white to gray
SMILES

O=C(NC1=CC=C(N2N=NC(CCCC)=C2)C=C1)NC3=CC=CC=C3C
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (286.18 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.8618 mL

14.3090 mL

28.6180 mL

5 mM

0.5724 mL

2.8618 mL

5.7236 mL

10 mM

0.2862 mL

1.4309 mL

2.8618 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (7.15 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Brai A, et al. Human DDX3 protein is a valuable target to develop broad spectrum antiviral agents. Proc Natl Acad Sci U S A. 2016;113(19):5388-5393. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.8618 mL	14.3090 mL	28.6180 mL	71.5451 mL
	5 mM	0.5724 mL	2.8618 mL	5.7236 mL	14.3090 mL
	10 mM	0.2862 mL	1.4309 mL	2.8618 mL	7.1545 mL
	15 mM	0.1908 mL	0.9539 mL	1.9079 mL	4.7697 mL
	20 mM	0.1431 mL	0.7155 mL	1.4309 mL	3.5773 mL
	25 mM	0.1145 mL	0.5724 mL	1.1447 mL	2.8618 mL
	30 mM	0.0954 mL	0.4770 mL	0.9539 mL	2.3848 mL
	40 mM	0.0715 mL	0.3577 mL	0.7155 mL	1.7886 mL
	50 mM	0.0572 mL	0.2862 mL	0.5724 mL	1.4309 mL
	60 mM	0.0477 mL	0.2385 mL	0.4770 mL	1.1924 mL
	80 mM	0.0358 mL	0.1789 mL	0.3577 mL	0.8943 mL
	100 mM	0.0286 mL	0.1431 mL	0.2862 mL	0.7155 mL

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DDX3-IN-2

Anti-Infection Compound Library

Antiviral Compound Library

Anti-Drug-Resistant Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Alle HIV Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

DDX3-IN-2

Anti-Infection Compound Library

Antiviral Compound Library

Anti-Drug-Resistant Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Bioactive Compound Library

High-Throughput Bioactive Compound Library

Alle HIV Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

DDX3-IN-2 Related Classifications

HY-121969 Related Classifications