IL-6 Protein, Mouse (His)
Based on 1 Customer Validation
IL-6 Protein is a multifunctional cytokine secreted by both immune and non-immune cells, and belongs to the interleukin family. IL-6 Protein plays a key role in immune responses, inflammatory reactions and other processes. IL-6 Protein, Mouse (His) is a recombinant IL-6 protein expressed by E. coli with a N-6*His tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
IL-6 Protein is a multifunctional cytokine secreted by both immune and non-immune cells, and belongs to the interleukin family. IL-6 Protein plays a key role in immune responses, inflammatory reactions and other processes. IL-6 Protein, Mouse (His) is a recombinant IL-6 protein expressed by E. coli with a N-6*His tag[1][2][3].
IL-6 is a multifunctional cytokine secreted by immune cells (such as T cells, B cells, and macrophages) and non-immune cells (such as fibroblasts, endothelial cells, and hepatocytes). IL-6 transmits signals by binding to its receptor. As a cytokine rapidly and transiently produced in response to infection and tissue damage, IL-6 participates in host defense by stimulating acute-phase responses, hematopoiesis, and immune reactions. Its dysregulation can trigger chronic inflammation and autoimmune diseases. In the initial stage of inflammation, IL-6 is transported via blood to the liver to induce the synthesis of multiple acute-phase proteins. It also participates in regulating serum iron and zinc levels and platelet release. In immune responses, IL-6 promotes Th17 differentiation, inhibits Treg differentiation, and affects immune tolerance[1][2][3].
IL-6 Protein (Mouse; 25 ng/mL; 24 h) can promote the production of inflammatory factors MCP-1, MMP-3, CCL5, CXCL12 and the expression of hypertrophic markers COL10A1 and RUNX2 in primary mouse chondrocytes. The mechanism involves the activation of the JAK2/STAT3 pathway[3].
IL-6 Protein (Mouse; 0.3 μg/kg; intra-articular injection; single dose) can cause spinal hyperexcitability in rats[3].
Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is ≤346.9 pg/mL, corresponding to a specific activity is ≥2.88×106 units/mg.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-6*His
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Accession
P08505 (F25-T211)
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Molecular Construction
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N-term
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6*His
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IL-6 (F25-T211)
Accession # P08505 -
C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
IL6; Hybridoma Growth Factor; Prev. IFNB2; IFN-Beta-2; IL-6; BSF-2; Interleukin-6; CDF; BSF2; Interleukin 6 (Interferon, Beta 2); HGF; B-Cell Differentiation Factor; HSF; Truncated Interleukin 6; B-Cell Stimulatory Factor 2; Interferon, Beta 2; CTL Differ
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AA Sequence
FPTSQVRRGDFTEDTTPNRPVYTTSQVGGLITHVLWEIVEMRKELCNGNSDCMNNDDALAENNLKLPEIQRNDGCYQTGYNQEICLLKISSGLLEYHSYLEYMKNNLKDNKKDKARVLQRDTETLIHIFNQEVKDLHKIVLPTPISNALLTDKLESQKEWLRTKTIQFILKSLEEFLKVTLRSTRQT
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Predicted Molecular Mass
21.7 kDa
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Molecular Weight
Approximately 22 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4 or 20 mM Sodium Acetate, pH 4.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Frorath B, et al. Production of recombinant rat interleukin-6 in Escherichia coli using a novel highly efficient expression vector pGEX-3T. Biotechniques. 1992 Apr;12(4):558-63. [Content Brief]
[2]. Tanaka T, et al. IL-6 in inflammation, immunity, and disease. Cold Spring Harb Perspect Biol. 2014 Sep 4;6(10):a016295. [Content Brief]
[3]. Mima Z, et al. Blockade of JAK2 retards cartilage degeneration and IL-6-induced pain amplification in osteoarthritis. Int Immunopharmacol. 2022 Dec;113(Pt A):109340. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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