2. Metabolic Enzyme/Protease
  3. Amino Acid Oxidase
  4. DAO-IN-2

DAO-IN-2 is a d-amino acid oxidase (DAO) inhibitor (h-DAO IC50 = 245 nM). DAO-IN-2 is a moderate inhibitor of DAAO in vitro and in vivo. DAO-IN-2 can be used to study DAAO-related psychiatric disorders.

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DAO-IN-2

DAO-IN-2 構造式

CAS 番号 : 39793-31-2

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 27 在庫あり
Solution
10 mM * 1 mL in DMSO USD 27 在庫あり
Solid
100 mg $25 在庫あり
250 mg $40 在庫あり
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1 g   お問い合わせ  

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Based on 1 publication(s) in Google Scholar

Other Forms of DAO-IN-2:

DAO-IN-2 関連抗体

Top Publications Citing Use of Products

Amino Acid Oxidase アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
D-Amino acid oxidase l-Amino acid oxidase

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製品説明

DAO-IN-2 is a d-amino acid oxidase (DAO) inhibitor (h-DAO IC50 = 245 nM). DAO-IN-2 is a moderate inhibitor of DAAO in vitro and in vivo. DAO-IN-2 can be used to study DAAO-related psychiatric disorders[1][2].

体外実験

DAO-IN-2 (Compound 8) has no off-target activities for the inhibition of P450 enzymes (IC50s > 10 μM for CYP3A4, CYP2D6 and CYP3C9) or binding to ion channels[1].
DAO-IN-2 inhibits D-amino acid oxidase (DAAO) activity against expressing either the human or rat DAAO gene CHO cells, with IC50s of 144.6, 113.9 nM[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DAO-IN-2 関連抗体
体内実験

DAO-IN-2 (Compound 8) (10-200 mg/kg, i.p., once) inhibits DAAO activity in the kidney and cerebellum, increases plasma and cerebrospinal fluid D-serine levels, and reduces amphetamine-induced psychomotor spontaneous activity in Wistar rats model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Wistar rats (adult male) model[2]
Dosage: 10, 25, 50, 100, 200 mg/kg
Administration: i.p., once
Result: Inhibited kidney DAAO activity both time- and dose-dependent, elevated plasma D-serine levels, did not alter circulating levels of glycine or D-alanine.
Inhibited DAAO activity in the cerebellum, increased CSF levels of D-serine.
Reduced spontaneous activity on amphetamine-induced psychomotor activity.
分子量

167.19

分子式

C7H5NO2S
CAS 番号
Appearance

Solid

SMILES

O=C(C1=CC2=C(C=CS2)N1)O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (598.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.9812 mL 29.9061 mL 59.8122 mL
5 mM 1.1962 mL 5.9812 mL 11.9624 mL
10 mM 0.5981 mL 2.9906 mL 5.9812 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

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体積 （開始）

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体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (7.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (7.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
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Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.95%

COA (250 KB) HNMR (132 KB) RP-HPLC (225 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 5.9812 mL 29.9061 mL 59.8122 mL 149.5305 mL
5 mM 1.1962 mL 5.9812 mL 11.9624 mL 29.9061 mL
10 mM 0.5981 mL 2.9906 mL 5.9812 mL 14.9530 mL
15 mM 0.3987 mL 1.9937 mL 3.9875 mL 9.9687 mL
20 mM 0.2991 mL 1.4953 mL 2.9906 mL 7.4765 mL
25 mM 0.2392 mL 1.1962 mL 2.3925 mL 5.9812 mL
30 mM 0.1994 mL 0.9969 mL 1.9937 mL 4.9843 mL
40 mM 0.1495 mL 0.7477 mL 1.4953 mL 3.7383 mL
50 mM 0.1196 mL 0.5981 mL 1.1962 mL 2.9906 mL
60 mM 0.0997 mL 0.4984 mL 0.9969 mL 2.4922 mL
80 mM 0.0748 mL 0.3738 mL 0.7477 mL 1.8691 mL
100 mM 0.0598 mL 0.2991 mL 0.5981 mL 1.4953 mL
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DAO-IN-2 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DAO-IN-239793-31-2Amino Acid OxidaseDAO IhibitorInhibitorinhibitorinhibit

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製品番号:
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