Cat. No.: HY-N0413

Hupehenine

Customer Review

Based on 1 Customer Validation

Hupehenine is an orally active isosteroidal alkaloid that can be extracted from F. hupehensis. Hupehenine exhibits activities such as antitussive, expectorant, anticancer, and antiparasitic. In vitro, Hupehenine can also inhibit α-synuclein seeded fibril formation, making it applicable for the research of Parkinson's disease and other related disorders.

For research use only. We do not sell to patients.

Purity: 99.0%
CAS No.: 98243-57-3
Formula: C₂₇H₄₅NO₂
Molecular Weight:415.65

Storage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Hupehenine Chemical Structure

CAS No.: 98243-57-3

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All α-synuclein Isoforms :

All Parasite Isoforms :

In Vitro

Hupehenine (24 h) can inhibit α-synuclein seeded fibril formation in vitro^[1].
Hupehenine (2-10 µM; 48 h) does not significantly reduce α-synuclein seed-induced cytotoxicity in SH-SY5Y cells^[1].
Hupehenine (7.2-14.4 µM; 48 h) can induce the elevation of ROS levels, loss of mitochondrial membrane potential, cell cycle arrest, as well as autophagy and apoptosis in Leishmania donovani promastigotes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[2]

Cell Line:

Leishmania donovani
Concentration:

7.2 µM
Incubation Time:

48 h
Result:

Increased the levels of major autophagic genes ATG7 and ATG8B.

In Vivo

Pharmacokinetic Analysis in Rats^[3]

Route	Dose (mg/kg)	AUC_0-t (ng/mL h)	AUC_0-∞ (ng/mL h)	t_1/2 (h)	CL (L/h/kg)	V (L/kg)	C_max (ng/mL)	Bioavailability, F
p.o.	10	935.6	1097.4	5.4	11.5	91.5	390.2	13.4%
i.v.	5	3494.9	3514.3	4..6	1.5	9.9	1400.5	13.4%

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 98243-57-3
Appearance Solid
Molecular Weight 415.65
Formula C₂₇H₄₅NO₂
Color White to off-white
SMILES

O[C@H]1CC[C@@]2(C)[C@]([C@H](O)C[C@]3([H])[C@]2([H])C[C@]4([H])[C@@]3([H])CC[C@]5([H])[C@@]4([H])CN6[C@](CC[C@H](C)C6)([H])[C@@H]5C)([H])C1
Structure Classification
- Alkaloids
- Indole Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (240.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4059 mL

12.0294 mL

24.0587 mL

5 mM

0.4812 mL

2.4059 mL

4.8117 mL

10 mM

0.2406 mL

1.2029 mL

2.4059 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Ghanem SS, et al. Natural Alkaloid Compounds as Inhibitors for Alpha-Synuclein Seeded Fibril Formation and Toxicity. Molecules. 2021 Jun 19;26(12):3736. [Content Brief]

[2]. Menpadi N, et al. Molecular Mechanisms of Cell Death in Leishmania donovani Induced by Selected Steroidal Alkaloids. J Basic Microbiol. 2025 Mar;65(3):e2400655. [Content Brief]

[3]. Wen C, et al. Determination and validation of hupehenine in rat plasma by UPLC-MS/MS and its application to pharmacokinetic study. Biomed Chromatogr. 2015 Dec;29(12):1805-10. [Content Brief]

[4]. Wu X, et al. Investigation of association of chemical profiles with the tracheobronchial relaxant activity of Chinese medicinal herb Beimu derived from various Fritillaria species. J Ethnopharmacol. 2018 Jan 10;210:39-46. [Content Brief]

[5]. Xin M, et al. Design, synthesis, and evaluation of pyrrolo[2,1-f][1,2,4]triazine derivatives as novel hedgehog signaling pathway inhibitors. Bioorg Med Chem. 2014 Feb 15;22(4):1429-40. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4059 mL	12.0294 mL	24.0587 mL	60.1468 mL
	5 mM	0.4812 mL	2.4059 mL	4.8117 mL	12.0294 mL
	10 mM	0.2406 mL	1.2029 mL	2.4059 mL	6.0147 mL
	15 mM	0.1604 mL	0.8020 mL	1.6039 mL	4.0098 mL
	20 mM	0.1203 mL	0.6015 mL	1.2029 mL	3.0073 mL
	25 mM	0.0962 mL	0.4812 mL	0.9623 mL	2.4059 mL
	30 mM	0.0802 mL	0.4010 mL	0.8020 mL	2.0049 mL
	40 mM	0.0601 mL	0.3007 mL	0.6015 mL	1.5037 mL
	50 mM	0.0481 mL	0.2406 mL	0.4812 mL	1.2029 mL
	60 mM	0.0401 mL	0.2005 mL	0.4010 mL	1.0024 mL
	80 mM	0.0301 mL	0.1504 mL	0.3007 mL	0.7518 mL
	100 mM	0.0241 mL	0.1203 mL	0.2406 mL	0.6015 mL

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Hupehenine

Orally Active Compound Library

Traditional Chinese Medicine Active Compound Library

Alkaloids Library

Antiparasitic Compound library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Heterocyclic Compound Library

Cell Death Library

Multi-Target Compound Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Bioactive Compound Library

Tibetan Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Natural Product Diversity Scaffold Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Natural Product and Natural Product-Like Compound Library

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

Neuronal Signaling Compound Library

NF-κB Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Autophagy Compound Library

Anti-Aging Compound Library

All α-synuclein Isoforms

All Parasite Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Hupehenine Related Classifications

HY-N0413 Related Classifications