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  4. Dodecyl β-D-glucopyranoside

Dodecyl β-D-glucopyranoside is a non-ionic detergent and surfactant commonly used to solubilize and purify membrane proteins in biochemical research. Dodecyl β-D-glucopyranoside also interacts with bovine serum albumin (BSA) to quench its intrinsic fluorescence. The critical micelle concentration (CMC) of Dodecyl β-D-glucopyranoside (DG) and DG/BSA complex is 2.0 mM and 2.34 mM, respectively. Micelles can be formed in aqueous solutions above this concentration.

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Dodecyl β-D-glucopyranoside

Dodecyl β-D-glucopyranoside Chemical Structure

CAS No. : 59122-55-3

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10 mM * 1 mL in DMSO
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Based on 1 publication(s) in Google Scholar

Dodecyl β-D-glucopyranoside Related Antibodies

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Description

Dodecyl β-D-glucopyranoside is a non-ionic detergent and surfactant commonly used to solubilize and purify membrane proteins in biochemical research. Dodecyl β-D-glucopyranoside also interacts with bovine serum albumin (BSA) to quench its intrinsic fluorescence. The critical micelle concentration (CMC) of Dodecyl β-D-glucopyranoside (DG) and DG/BSA complex is 2.0 mM and 2.34 mM, respectively. Micelles can be formed in aqueous solutions above this concentration[1].

Molecular Weight

348.47

Formula

C18H36O6
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCCCCO[C@H]1[C@@H]([C@H]([C@@H]([C@@H](CO)O1)O)O)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (286.97 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.8697 mL 14.3484 mL 28.6969 mL
5 mM 0.5739 mL 2.8697 mL 5.7394 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration
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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.8697 mL 14.3484 mL 28.6969 mL 71.7422 mL
5 mM 0.5739 mL 2.8697 mL 5.7394 mL 14.3484 mL
10 mM 0.2870 mL 1.4348 mL 2.8697 mL 7.1742 mL
15 mM 0.1913 mL 0.9566 mL 1.9131 mL 4.7828 mL
20 mM 0.1435 mL 0.7174 mL 1.4348 mL 3.5871 mL
25 mM 0.1148 mL 0.5739 mL 1.1479 mL 2.8697 mL
30 mM 0.0957 mL 0.4783 mL 0.9566 mL 2.3914 mL
40 mM 0.0717 mL 0.3587 mL 0.7174 mL 1.7936 mL
50 mM 0.0574 mL 0.2870 mL 0.5739 mL 1.4348 mL
60 mM 0.0478 mL 0.2391 mL 0.4783 mL 1.1957 mL
80 mM 0.0359 mL 0.1794 mL 0.3587 mL 0.8968 mL
100 mM 0.0287 mL 0.1435 mL 0.2870 mL 0.7174 mL
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Dodecyl β-D-glucopyranoside Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Dodecyl β-D-glucopyranoside59122-55-3Biochemical Assay ReagentsInhibitorinhibitorinhibit

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